Optimal conditions for palmitate oxidation by rat heart homogenates

Susana Passeron, Michael A. Savageau, Isaac Harary

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

A study of the oxidation of palmitate-1-C14 to C14O2 by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for carnitine, CoA, ATP, and Mg2+. The levels of ATP and Mg2+ for the optimal rate of palmitate oxidation are interdependent. Excess of ATP or Mg2+ inhibits the production of CO2. The amount of fatty acid oxidized is dependent on the molar ratio of fatty acid to albumin in the incubation medium: an optimal ratio of approximately 5 was found for all the concentrations of fatty acid and albumin tested. A tentative mechanism for the albumin effect is presented.

Original languageEnglish (US)
Pages (from-to)124-128
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume128
Issue number1
StatePublished - Oct 1968

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

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    Passeron, S., Savageau, M. A., & Harary, I. (1968). Optimal conditions for palmitate oxidation by rat heart homogenates. Archives of Biochemistry and Biophysics, 128(1), 124-128.