Abstract
A study of the oxidation of palmitate-1-C14 to C14O2 by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for carnitine, CoA, ATP, and Mg2+. The levels of ATP and Mg2+ for the optimal rate of palmitate oxidation are interdependent. Excess of ATP or Mg2+ inhibits the production of CO2. The amount of fatty acid oxidized is dependent on the molar ratio of fatty acid to albumin in the incubation medium: an optimal ratio of approximately 5 was found for all the concentrations of fatty acid and albumin tested. A tentative mechanism for the albumin effect is presented.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 124-128 |
| Number of pages | 5 |
| Journal | Archives of Biochemistry and Biophysics |
| Volume | 128 |
| Issue number | 1 |
| State | Published - Oct 1968 |
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ASJC Scopus subject areas
- Molecular Biology
- Biophysics
- Biochemistry
Cite this
Optimal conditions for palmitate oxidation by rat heart homogenates. / Passeron, Susana; Savageau, Michael A.; Harary, Isaac.
In: Archives of Biochemistry and Biophysics, Vol. 128, No. 1, 10.1968, p. 124-128.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Optimal conditions for palmitate oxidation by rat heart homogenates
AU - Passeron, Susana
AU - Savageau, Michael A.
AU - Harary, Isaac
PY - 1968/10
Y1 - 1968/10
N2 - A study of the oxidation of palmitate-1-C14 to C14O2 by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for carnitine, CoA, ATP, and Mg2+. The levels of ATP and Mg2+ for the optimal rate of palmitate oxidation are interdependent. Excess of ATP or Mg2+ inhibits the production of CO2. The amount of fatty acid oxidized is dependent on the molar ratio of fatty acid to albumin in the incubation medium: an optimal ratio of approximately 5 was found for all the concentrations of fatty acid and albumin tested. A tentative mechanism for the albumin effect is presented.
AB - A study of the oxidation of palmitate-1-C14 to C14O2 by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for carnitine, CoA, ATP, and Mg2+. The levels of ATP and Mg2+ for the optimal rate of palmitate oxidation are interdependent. Excess of ATP or Mg2+ inhibits the production of CO2. The amount of fatty acid oxidized is dependent on the molar ratio of fatty acid to albumin in the incubation medium: an optimal ratio of approximately 5 was found for all the concentrations of fatty acid and albumin tested. A tentative mechanism for the albumin effect is presented.
UR - http://www.scopus.com/inward/record.url?scp=0014342520&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0014342520&partnerID=8YFLogxK
M3 - Article
C2 - 5677171
AN - SCOPUS:0014342520
VL - 128
SP - 124
EP - 128
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
SN - 0003-9861
IS - 1
ER -