Optimal conditions for palmitate oxidation by rat heart homogenates

Susana Passeron; Michael A. Savageau; Isaac Harary

Optimal conditions for palmitate oxidation by rat heart homogenates

Susana Passeron, Michael A. Savageau, Isaac Harary

Biomedical Engineering

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A study of the oxidation of palmitate-1-C¹⁴ to C¹⁴O₂ by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for carnitine, CoA, ATP, and Mg²⁺. The levels of ATP and Mg²⁺ for the optimal rate of palmitate oxidation are interdependent. Excess of ATP or Mg²⁺ inhibits the production of CO₂. The amount of fatty acid oxidized is dependent on the molar ratio of fatty acid to albumin in the incubation medium: an optimal ratio of approximately 5 was found for all the concentrations of fatty acid and albumin tested. A tentative mechanism for the albumin effect is presented.

Original language	English (US)
Pages (from-to)	124-128
Number of pages	5
Journal	Archives of Biochemistry and Biophysics
Volume	128
Issue number	1
State	Published - Oct 1968

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry

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title = "Optimal conditions for palmitate oxidation by rat heart homogenates",

abstract = "A study of the oxidation of palmitate-1-C14 to C14O2 by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for carnitine, CoA, ATP, and Mg2+. The levels of ATP and Mg2+ for the optimal rate of palmitate oxidation are interdependent. Excess of ATP or Mg2+ inhibits the production of CO2. The amount of fatty acid oxidized is dependent on the molar ratio of fatty acid to albumin in the incubation medium: an optimal ratio of approximately 5 was found for all the concentrations of fatty acid and albumin tested. A tentative mechanism for the albumin effect is presented.",

author = "Susana Passeron and Savageau, {Michael A.} and Isaac Harary",

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AU - Savageau, Michael A.

AU - Harary, Isaac

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N2 - A study of the oxidation of palmitate-1-C14 to C14O2 by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for carnitine, CoA, ATP, and Mg2+. The levels of ATP and Mg2+ for the optimal rate of palmitate oxidation are interdependent. Excess of ATP or Mg2+ inhibits the production of CO2. The amount of fatty acid oxidized is dependent on the molar ratio of fatty acid to albumin in the incubation medium: an optimal ratio of approximately 5 was found for all the concentrations of fatty acid and albumin tested. A tentative mechanism for the albumin effect is presented.

AB - A study of the oxidation of palmitate-1-C14 to C14O2 by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for carnitine, CoA, ATP, and Mg2+. The levels of ATP and Mg2+ for the optimal rate of palmitate oxidation are interdependent. Excess of ATP or Mg2+ inhibits the production of CO2. The amount of fatty acid oxidized is dependent on the molar ratio of fatty acid to albumin in the incubation medium: an optimal ratio of approximately 5 was found for all the concentrations of fatty acid and albumin tested. A tentative mechanism for the albumin effect is presented.

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