Abstract
A study of the oxidation of palmitate-1-C14 to C14O2 by heart homogenates was undertaken and the optimal conditions were determined. The system has an absolute requirement for carnitine, CoA, ATP, and Mg2+. The levels of ATP and Mg2+ for the optimal rate of palmitate oxidation are interdependent. Excess of ATP or Mg2+ inhibits the production of CO2. The amount of fatty acid oxidized is dependent on the molar ratio of fatty acid to albumin in the incubation medium: an optimal ratio of approximately 5 was found for all the concentrations of fatty acid and albumin tested. A tentative mechanism for the albumin effect is presented.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 124-128 |
| Number of pages | 5 |
| Journal | Archives of Biochemistry and Biophysics |
| Volume | 128 |
| Issue number | 1 |
| State | Published - Oct 1968 |
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ASJC Scopus subject areas
- Molecular Biology
- Biophysics
- Biochemistry
Cite this
Passeron, S., Savageau, M. A., & Harary, I. (1968). Optimal conditions for palmitate oxidation by rat heart homogenates. Archives of Biochemistry and Biophysics, 128(1), 124-128.