Optical spectroscopy and imaging of the dentin-enamel junction in human third molars

R. R. Gallagher, S. G. Demos, M. Balooch, G. W. Marshall, S. J. Marshall

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


A 351-nm laser excitation source was used to perform autofluorescence microscopy of dentin, enamel, and the dentin-enamel junction (DEJ) to obtain information regarding their morphology and spectral characteristics. The emission spectra of these calcified dental tissues were different from one another, and this enabled the DEJ to be imaged and dimensionalized. The DEJ displayed sharp and clearly delineated borders at both its enamel and dentin margins. The dentinal tubules and the enamel prisms appeared to terminate abruptly at the DEJ. The median DEJ width was 10 μm, ranging from 7 to 15 μm, and it did not appear to depend on intratooth position.

Original languageEnglish (US)
Pages (from-to)372-377
Number of pages6
JournalJournal of Biomedical Materials Research - Part A
Issue number2
StatePublished - Feb 1 2003
Externally publishedYes


  • Biological interfacial material
  • Dentin-enamel junction (DEJ)
  • Emission spectroscopy
  • Fluorescence spectra
  • Width estimate

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials


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