Optical scatter imaging of programmed cell death

Wilsaan Joiner, N. N. Boustany, N. V. Thakor

Research output: Contribution to conferencePaper

Abstract

Optical Scatter Imaging (OSI) was recently developed in our laboratory to study subcellular morphological changes in viable cells. The process utilizes Fourier filtering and the ratio of wide-to-narrow angle scatter intensity (OSIR) to detect alterations in the size of particles with wavelength-scale dimensions. In the present study, we utilize OSI to track subcellular changes during programmed cell death. OSI showed an average 21% decrease in OSIR. The OSIR initial was 183.8 +/- 7.2 and the OSIR final was 145.9 +/- 4.3, p < 0.001, n=57 (mean +/- 95% confidence interval of the mean) upon induction of cell death by 1μM of the kinase inhibitor staurosporine. This decrease was not observed in cells that over expressed Bcl-x, an anti-apoptotic protein localized on the mitochondria. Combining OSI with genetic manipulations provides a novel approach and tool, with which to study apoptosis, a fundamental biological process.

Original languageEnglish (US)
Pages127-128
Number of pages2
StatePublished - Jan 1 2002
Externally publishedYes
EventIEEE 28th Annual Northeast Bioengineering Conference - Philadelphia, PA, United States
Duration: Apr 20 2002Apr 21 2002

Other

OtherIEEE 28th Annual Northeast Bioengineering Conference
CountryUnited States
CityPhiladelphia, PA
Period4/20/024/21/02

Fingerprint

Cell death
Imaging techniques
Apoptosis Regulatory Proteins
Mitochondria
Staurosporine
Phosphotransferases
Apoptosis
Proteins
Wavelength

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

Joiner, W., Boustany, N. N., & Thakor, N. V. (2002). Optical scatter imaging of programmed cell death. 127-128. Paper presented at IEEE 28th Annual Northeast Bioengineering Conference, Philadelphia, PA, United States.

Optical scatter imaging of programmed cell death. / Joiner, Wilsaan; Boustany, N. N.; Thakor, N. V.

2002. 127-128 Paper presented at IEEE 28th Annual Northeast Bioengineering Conference, Philadelphia, PA, United States.

Research output: Contribution to conferencePaper

Joiner, W, Boustany, NN & Thakor, NV 2002, 'Optical scatter imaging of programmed cell death' Paper presented at IEEE 28th Annual Northeast Bioengineering Conference, Philadelphia, PA, United States, 4/20/02 - 4/21/02, pp. 127-128.
Joiner W, Boustany NN, Thakor NV. Optical scatter imaging of programmed cell death. 2002. Paper presented at IEEE 28th Annual Northeast Bioengineering Conference, Philadelphia, PA, United States.
Joiner, Wilsaan ; Boustany, N. N. ; Thakor, N. V. / Optical scatter imaging of programmed cell death. Paper presented at IEEE 28th Annual Northeast Bioengineering Conference, Philadelphia, PA, United States.2 p.
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