Optical scatter imaging of programmed cell death

Wilsaan Joiner; N. N. Boustany; N. V. Thakor

Optical scatter imaging of programmed cell death

Wilsaan Joiner, N. N. Boustany, N. V. Thakor

Research output: Contribution to conference › Paper

Abstract

Optical Scatter Imaging (OSI) was recently developed in our laboratory to study subcellular morphological changes in viable cells. The process utilizes Fourier filtering and the ratio of wide-to-narrow angle scatter intensity (OSIR) to detect alterations in the size of particles with wavelength-scale dimensions. In the present study, we utilize OSI to track subcellular changes during programmed cell death. OSI showed an average 21% decrease in OSIR. The OSIR initial was 183.8 +/- 7.2 and the OSIR final was 145.9 +/- 4.3, p < 0.001, n=57 (mean +/- 95% confidence interval of the mean) upon induction of cell death by 1μM of the kinase inhibitor staurosporine. This decrease was not observed in cells that over expressed Bcl-x, an anti-apoptotic protein localized on the mitochondria. Combining OSI with genetic manipulations provides a novel approach and tool, with which to study apoptosis, a fundamental biological process.

Original language	English (US)
Pages	127-128
Number of pages	2
State	Published - Jan 1 2002
Externally published	Yes
Event	IEEE 28th Annual Northeast Bioengineering Conference - Philadelphia, PA, United States Duration: Apr 20 2002 → Apr 21 2002

Other

Other	IEEE 28th Annual Northeast Bioengineering Conference
Country	United States
City	Philadelphia, PA
Period	4/20/02 → 4/21/02

ASJC Scopus subject areas

Chemical Engineering(all)

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Joiner, W., Boustany, N. N., & Thakor, N. V. (2002). Optical scatter imaging of programmed cell death. 127-128. Paper presented at IEEE 28th Annual Northeast Bioengineering Conference, Philadelphia, PA, United States.

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abstract = "Optical Scatter Imaging (OSI) was recently developed in our laboratory to study subcellular morphological changes in viable cells. The process utilizes Fourier filtering and the ratio of wide-to-narrow angle scatter intensity (OSIR) to detect alterations in the size of particles with wavelength-scale dimensions. In the present study, we utilize OSI to track subcellular changes during programmed cell death. OSI showed an average 21% decrease in OSIR. The OSIR initial was 183.8 +/- 7.2 and the OSIR final was 145.9 +/- 4.3, p < 0.001, n=57 (mean +/- 95% confidence interval of the mean) upon induction of cell death by 1μM of the kinase inhibitor staurosporine. This decrease was not observed in cells that over expressed Bcl-x, an anti-apoptotic protein localized on the mitochondria. Combining OSI with genetic manipulations provides a novel approach and tool, with which to study apoptosis, a fundamental biological process.",

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AU - Joiner, Wilsaan

AU - Boustany, N. N.

AU - Thakor, N. V.

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N2 - Optical Scatter Imaging (OSI) was recently developed in our laboratory to study subcellular morphological changes in viable cells. The process utilizes Fourier filtering and the ratio of wide-to-narrow angle scatter intensity (OSIR) to detect alterations in the size of particles with wavelength-scale dimensions. In the present study, we utilize OSI to track subcellular changes during programmed cell death. OSI showed an average 21% decrease in OSIR. The OSIR initial was 183.8 +/- 7.2 and the OSIR final was 145.9 +/- 4.3, p < 0.001, n=57 (mean +/- 95% confidence interval of the mean) upon induction of cell death by 1μM of the kinase inhibitor staurosporine. This decrease was not observed in cells that over expressed Bcl-x, an anti-apoptotic protein localized on the mitochondria. Combining OSI with genetic manipulations provides a novel approach and tool, with which to study apoptosis, a fundamental biological process.

AB - Optical Scatter Imaging (OSI) was recently developed in our laboratory to study subcellular morphological changes in viable cells. The process utilizes Fourier filtering and the ratio of wide-to-narrow angle scatter intensity (OSIR) to detect alterations in the size of particles with wavelength-scale dimensions. In the present study, we utilize OSI to track subcellular changes during programmed cell death. OSI showed an average 21% decrease in OSIR. The OSIR initial was 183.8 +/- 7.2 and the OSIR final was 145.9 +/- 4.3, p < 0.001, n=57 (mean +/- 95% confidence interval of the mean) upon induction of cell death by 1μM of the kinase inhibitor staurosporine. This decrease was not observed in cells that over expressed Bcl-x, an anti-apoptotic protein localized on the mitochondria. Combining OSI with genetic manipulations provides a novel approach and tool, with which to study apoptosis, a fundamental biological process.

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