Abstract
Optical Scatter Imaging (OSI) was recently developed in our laboratory to study subcellular morphological changes in viable cells. The process utilizes Fourier filtering and the ratio of wide-to-narrow angle scatter intensity (OSIR) to detect alterations in the size of particles with wavelength-scale dimensions. In the present study, we utilize OSI to track subcellular changes during programmed cell death. OSI showed an average 21% decrease in OSIR. The OSIR initial was 183.8 +/- 7.2 and the OSIR final was 145.9 +/- 4.3, p < 0.001, n=57 (mean +/- 95% confidence interval of the mean) upon induction of cell death by 1μM of the kinase inhibitor staurosporine. This decrease was not observed in cells that over expressed Bcl-x, an anti-apoptotic protein localized on the mitochondria. Combining OSI with genetic manipulations provides a novel approach and tool, with which to study apoptosis, a fundamental biological process.
| Original language | English (US) |
|---|---|
| Pages | 127-128 |
| Number of pages | 2 |
| State | Published - Jan 1 2002 |
| Externally published | Yes |
| Event | IEEE 28th Annual Northeast Bioengineering Conference - Philadelphia, PA, United States Duration: Apr 20 2002 → Apr 21 2002 |
Other
| Other | IEEE 28th Annual Northeast Bioengineering Conference |
|---|---|
| Country | United States |
| City | Philadelphia, PA |
| Period | 4/20/02 → 4/21/02 |
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ASJC Scopus subject areas
- Chemical Engineering(all)
Cite this
Optical scatter imaging of programmed cell death. / Joiner, Wilsaan; Boustany, N. N.; Thakor, N. V.
2002. 127-128 Paper presented at IEEE 28th Annual Northeast Bioengineering Conference, Philadelphia, PA, United States.Research output: Contribution to conference › Paper
}
TY - CONF
T1 - Optical scatter imaging of programmed cell death
AU - Joiner, Wilsaan
AU - Boustany, N. N.
AU - Thakor, N. V.
PY - 2002/1/1
Y1 - 2002/1/1
N2 - Optical Scatter Imaging (OSI) was recently developed in our laboratory to study subcellular morphological changes in viable cells. The process utilizes Fourier filtering and the ratio of wide-to-narrow angle scatter intensity (OSIR) to detect alterations in the size of particles with wavelength-scale dimensions. In the present study, we utilize OSI to track subcellular changes during programmed cell death. OSI showed an average 21% decrease in OSIR. The OSIR initial was 183.8 +/- 7.2 and the OSIR final was 145.9 +/- 4.3, p < 0.001, n=57 (mean +/- 95% confidence interval of the mean) upon induction of cell death by 1μM of the kinase inhibitor staurosporine. This decrease was not observed in cells that over expressed Bcl-x, an anti-apoptotic protein localized on the mitochondria. Combining OSI with genetic manipulations provides a novel approach and tool, with which to study apoptosis, a fundamental biological process.
AB - Optical Scatter Imaging (OSI) was recently developed in our laboratory to study subcellular morphological changes in viable cells. The process utilizes Fourier filtering and the ratio of wide-to-narrow angle scatter intensity (OSIR) to detect alterations in the size of particles with wavelength-scale dimensions. In the present study, we utilize OSI to track subcellular changes during programmed cell death. OSI showed an average 21% decrease in OSIR. The OSIR initial was 183.8 +/- 7.2 and the OSIR final was 145.9 +/- 4.3, p < 0.001, n=57 (mean +/- 95% confidence interval of the mean) upon induction of cell death by 1μM of the kinase inhibitor staurosporine. This decrease was not observed in cells that over expressed Bcl-x, an anti-apoptotic protein localized on the mitochondria. Combining OSI with genetic manipulations provides a novel approach and tool, with which to study apoptosis, a fundamental biological process.
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M3 - Paper
SP - 127
EP - 128
ER -