Optical scatter imaging of programmed cell death

Wilsaan Joiner, N. N. Boustany, N. V. Thakor

Research output: Chapter in Book/Report/Conference proceedingConference contribution


Optical Scatter Imaging (OSI) was recently developed in our laboratory to study subcellular morphological changes in viable cells. The process utilizes Fourier filtering and the ratio of wide-to-narrow angle scatter intensity (OSIR) to detect alterations in the size of particles with wavelength-scale dimensions. In the present study, we utilize OSI to track subcellular changes during programmed cell death. OSI showed an average 21% decrease in OSIR. The OSIR initial was 183.8±7.2 and the OSIR final was 145.9±4.3, p<0.001, n=57 (mean ±95% confidence interval of the mean) upon induction of cell death by 1 μM of the kinase inhibitor staurosporine. This decrease was not observed in cells that over expressed Bcl-x, an antiapoptotic protein localized on the mitochondria. Combining OSI with genetic manipulations provides a novel approach and tool, with which to study apoptosis, a fundamental biological process.

Original languageEnglish (US)
Title of host publicationProceedings of the IEEE 28th Annual Northeast Bioengineering Conference, NEBC 2002
EditorsDalia El-Sherif, Karen Moxon, Saravanan Kanakasabai
PublisherInstitute of Electrical and Electronics Engineers Inc.
Number of pages2
ISBN (Electronic)0780374193
StatePublished - Jan 1 2002
Externally publishedYes
Event28th IEEE Annual Northeast Bioengineering Conference, NEBC 2002 - Philadelphia, United States
Duration: Apr 20 2002Apr 21 2002


Other28th IEEE Annual Northeast Bioengineering Conference, NEBC 2002
Country/TerritoryUnited States


  • Biomedical optical imaging
  • Filtering
  • Inhibitors
  • Laboratories
  • Open systems
  • Optical filters
  • Optical imaging
  • Optical scattering
  • Particle scattering
  • Proteins

ASJC Scopus subject areas

  • Bioengineering


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