Optical nanoscopy of a living cell

Balpreet Singh Ahluwalia, Deanna L. Wolfson, Frank Chuang, Thomas R Huser

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Optical nanoscopy allows to study biological and functional processes of sub-cellular organelles. In structured illumination microscopy (SIM) the sample is illuminated with a grid-like interference pattern to encode higher spatial frequency information into observable Moiré patterns. By acquiring multiple images and a computation trick a superresolved image is obtained. SIM provides resolution enhancement of 2X in each axis as compared to conventional microscopes. For a visible light, SIM provides an optical resolution of 100 nm. The challenges associated with optical nanoscopy of a living cell are photo-toxicity, special dye requirements and artifacts due to cell movement. SIM works with conventional dyes and is a wide-field technique making it suitable for imaging living cells. In this work, we will discuss the opportunities and challenges of imaging living cells using SIM. Two applications of optical nanoscopy of living cells will be discussed; a) imaging of mitochondria in a keratinocyte cell and Optical microscopy based on fluorescence has emerged as a vital tool in modern bio-medical imaging and diagnosis. Super-resolution bio-imaging allows gathering information from sub-cellular organelles. In structured illumination microscopy (SIM) the sample is illuminated with a grid-like interference patterns to encode higher spatial frequencies information into observable images (Moiré fringes). A super-resolved image is then decoded using computational trick. In this work, we used SIM to acquired super-resolved optical images of mitochondria from a live keratinocyte cell (see Fig 1). SIM provides resolution enhancement of 2X in each axis and contrast enhancement of 8X on a projected image. Time-lapsed imaging was used to study the dynamics of mitochondria in a live cell.

Original languageEnglish (US)
Title of host publicationNanoimaging and Nanospectroscopy II
PublisherSPIE
Volume9169
ISBN (Electronic)9781628411966
DOIs
StatePublished - 2014
EventNanoimaging and Nanospectroscopy II - San Diego, United States
Duration: Aug 17 2014Aug 19 2014

Other

OtherNanoimaging and Nanospectroscopy II
CountryUnited States
CitySan Diego
Period8/17/148/19/14

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    Ahluwalia, B. S., Wolfson, D. L., Chuang, F., & Huser, T. R. (2014). Optical nanoscopy of a living cell. In Nanoimaging and Nanospectroscopy II (Vol. 9169). [91690V] SPIE. https://doi.org/10.1117/12.2062581