Optical monitoring of oxygen tension in cortical microvessels with confocal microscopy

Mohammad A. Yaseen, Vivek Srinivasan, Sava Sakadžić, Weicheng Wu, Svetlana Ruvinskaya, Sergei A. Vinogradov, David A. Boas

Research output: Contribution to journalArticlepeer-review

43 Scopus citations


Evaluating cerebral oxygenation is of critical importance for the understanding of brain function and several neuropathologies. Although several techniques exist for measuring cerebral oxygenation in vivo, the most widely accepted techniques offer limited spatial resolution. We have developed a confocal imaging system for minimally invasive measurement of oxygen tension (pO2) in cerebral microvessels with high spatial and temporal resolution. The system relies on the phosphorescence quenching method using exogenous porphyrin-based dendritic oxygen probes. Here we present high-resolution phosphorescence images of cortical microvasculature and temporal pO2 profiles from multiple locations in response to varied fraction of inspired oxygen and functional activation.

Original languageEnglish (US)
Pages (from-to)22341-22350
Number of pages10
JournalOptics Express
Issue number25
StatePublished - Dec 7 2009
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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