Optical molecular imaging of epidermal growth factor receptor expression to improve detection of oral neoplasia

Nitin Nitin, Kelsey J. Rosbach, Adel El-Naggar, Michelle Williams, Ann Gillenwaterand, Rebecca R. Richards-Kortum

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

BACKGROUND: The development of noninvasive molecular imaging approaches has the potential to improve management of cancer. METHODS: In this study, we demonstrate the potential of noninvasive topical delivery of an epidermal growth factor-Alexa 647 (EGF-Alexa 647) conjugate to image changes in epidermal growth factor receptor expression associated with oral neoplasia. We report a series of preclinical analyses to evaluate the optical contrast achieved after topical delivery of EGF-Alexa 647 in a variety of model systems, including cells, three-dimensional tissue cultures, and intact human tissue specimens using wide-field and high-resolution fluorescence imaging. Data were collected from 17 different oral cancer patients: eight pairs of normal and abnormal biopsies and nine resected tumors were examined. RESULTS: The EGF-dye conjugate can be uniformly delivered throughout the oral epithelium with a penetration depth exceeding 500 μm and incubation time of less than 30 minutes. After EGF-Alexa 647 incubation, the presence of oral neoplasia is associated with a 1.5- to 6.9-fold increase in fluorescence contrast compared with grossly normal mucosa from the same patient with both wide-field and high-resolution fluorescence imaging. CONCLUSIONS: Results illustrate the potential of EGF-targeted fluorescent agents for in vivo molecular imaging, a technique that may aid in the diagnosis and characterization of oral neoplasia and allow real-time detection of tumor margins.

Original languageEnglish (US)
Pages (from-to)542-551
Number of pages10
JournalNeoplasia
Volume11
Issue number6
DOIs
StatePublished - Jun 2009
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research

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    Nitin, N., Rosbach, K. J., El-Naggar, A., Williams, M., Gillenwaterand, A., & Richards-Kortum, R. R. (2009). Optical molecular imaging of epidermal growth factor receptor expression to improve detection of oral neoplasia. Neoplasia, 11(6), 542-551. https://doi.org/10.1593/neo.09188