Optical molecular imaging of epidermal growth factor receptor expression to improve detection of oral neoplasia

Nitin Nitin, Kelsey J. Rosbach, Adel El-Naggar, Michelle Williams, Ann Gillenwaterand, Rebecca R. Richards-Kortum

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

BACKGROUND: The development of noninvasive molecular imaging approaches has the potential to improve management of cancer. METHODS: In this study, we demonstrate the potential of noninvasive topical delivery of an epidermal growth factor-Alexa 647 (EGF-Alexa 647) conjugate to image changes in epidermal growth factor receptor expression associated with oral neoplasia. We report a series of preclinical analyses to evaluate the optical contrast achieved after topical delivery of EGF-Alexa 647 in a variety of model systems, including cells, three-dimensional tissue cultures, and intact human tissue specimens using wide-field and high-resolution fluorescence imaging. Data were collected from 17 different oral cancer patients: eight pairs of normal and abnormal biopsies and nine resected tumors were examined. RESULTS: The EGF-dye conjugate can be uniformly delivered throughout the oral epithelium with a penetration depth exceeding 500 μm and incubation time of less than 30 minutes. After EGF-Alexa 647 incubation, the presence of oral neoplasia is associated with a 1.5- to 6.9-fold increase in fluorescence contrast compared with grossly normal mucosa from the same patient with both wide-field and high-resolution fluorescence imaging. CONCLUSIONS: Results illustrate the potential of EGF-targeted fluorescent agents for in vivo molecular imaging, a technique that may aid in the diagnosis and characterization of oral neoplasia and allow real-time detection of tumor margins.

Original languageEnglish (US)
Pages (from-to)542-551
Number of pages10
JournalNeoplasia
Volume11
Issue number6
DOIs
StatePublished - Jun 2009
Externally publishedYes

Fingerprint

Molecular Imaging
Optical Imaging
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Neoplasms
Oral Diagnosis
Mouth Neoplasms
Fluorescent Dyes
Mucous Membrane
Coloring Agents
Epithelium
Fluorescence
Biopsy

ASJC Scopus subject areas

  • Cancer Research

Cite this

Nitin, N., Rosbach, K. J., El-Naggar, A., Williams, M., Gillenwaterand, A., & Richards-Kortum, R. R. (2009). Optical molecular imaging of epidermal growth factor receptor expression to improve detection of oral neoplasia. Neoplasia, 11(6), 542-551. https://doi.org/10.1593/neo.09188

Optical molecular imaging of epidermal growth factor receptor expression to improve detection of oral neoplasia. / Nitin, Nitin; Rosbach, Kelsey J.; El-Naggar, Adel; Williams, Michelle; Gillenwaterand, Ann; Richards-Kortum, Rebecca R.

In: Neoplasia, Vol. 11, No. 6, 06.2009, p. 542-551.

Research output: Contribution to journalArticle

Nitin, N, Rosbach, KJ, El-Naggar, A, Williams, M, Gillenwaterand, A & Richards-Kortum, RR 2009, 'Optical molecular imaging of epidermal growth factor receptor expression to improve detection of oral neoplasia', Neoplasia, vol. 11, no. 6, pp. 542-551. https://doi.org/10.1593/neo.09188
Nitin N, Rosbach KJ, El-Naggar A, Williams M, Gillenwaterand A, Richards-Kortum RR. Optical molecular imaging of epidermal growth factor receptor expression to improve detection of oral neoplasia. Neoplasia. 2009 Jun;11(6):542-551. https://doi.org/10.1593/neo.09188
Nitin, Nitin ; Rosbach, Kelsey J. ; El-Naggar, Adel ; Williams, Michelle ; Gillenwaterand, Ann ; Richards-Kortum, Rebecca R. / Optical molecular imaging of epidermal growth factor receptor expression to improve detection of oral neoplasia. In: Neoplasia. 2009 ; Vol. 11, No. 6. pp. 542-551.
@article{c1dd5cd2be0542b1b75ecb3c5e3055a9,
title = "Optical molecular imaging of epidermal growth factor receptor expression to improve detection of oral neoplasia",
abstract = "BACKGROUND: The development of noninvasive molecular imaging approaches has the potential to improve management of cancer. METHODS: In this study, we demonstrate the potential of noninvasive topical delivery of an epidermal growth factor-Alexa 647 (EGF-Alexa 647) conjugate to image changes in epidermal growth factor receptor expression associated with oral neoplasia. We report a series of preclinical analyses to evaluate the optical contrast achieved after topical delivery of EGF-Alexa 647 in a variety of model systems, including cells, three-dimensional tissue cultures, and intact human tissue specimens using wide-field and high-resolution fluorescence imaging. Data were collected from 17 different oral cancer patients: eight pairs of normal and abnormal biopsies and nine resected tumors were examined. RESULTS: The EGF-dye conjugate can be uniformly delivered throughout the oral epithelium with a penetration depth exceeding 500 μm and incubation time of less than 30 minutes. After EGF-Alexa 647 incubation, the presence of oral neoplasia is associated with a 1.5- to 6.9-fold increase in fluorescence contrast compared with grossly normal mucosa from the same patient with both wide-field and high-resolution fluorescence imaging. CONCLUSIONS: Results illustrate the potential of EGF-targeted fluorescent agents for in vivo molecular imaging, a technique that may aid in the diagnosis and characterization of oral neoplasia and allow real-time detection of tumor margins.",
author = "Nitin Nitin and Rosbach, {Kelsey J.} and Adel El-Naggar and Michelle Williams and Ann Gillenwaterand and Richards-Kortum, {Rebecca R.}",
year = "2009",
month = "6",
doi = "10.1593/neo.09188",
language = "English (US)",
volume = "11",
pages = "542--551",
journal = "Neoplasia (United States)",
issn = "1522-8002",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Optical molecular imaging of epidermal growth factor receptor expression to improve detection of oral neoplasia

AU - Nitin, Nitin

AU - Rosbach, Kelsey J.

AU - El-Naggar, Adel

AU - Williams, Michelle

AU - Gillenwaterand, Ann

AU - Richards-Kortum, Rebecca R.

PY - 2009/6

Y1 - 2009/6

N2 - BACKGROUND: The development of noninvasive molecular imaging approaches has the potential to improve management of cancer. METHODS: In this study, we demonstrate the potential of noninvasive topical delivery of an epidermal growth factor-Alexa 647 (EGF-Alexa 647) conjugate to image changes in epidermal growth factor receptor expression associated with oral neoplasia. We report a series of preclinical analyses to evaluate the optical contrast achieved after topical delivery of EGF-Alexa 647 in a variety of model systems, including cells, three-dimensional tissue cultures, and intact human tissue specimens using wide-field and high-resolution fluorescence imaging. Data were collected from 17 different oral cancer patients: eight pairs of normal and abnormal biopsies and nine resected tumors were examined. RESULTS: The EGF-dye conjugate can be uniformly delivered throughout the oral epithelium with a penetration depth exceeding 500 μm and incubation time of less than 30 minutes. After EGF-Alexa 647 incubation, the presence of oral neoplasia is associated with a 1.5- to 6.9-fold increase in fluorescence contrast compared with grossly normal mucosa from the same patient with both wide-field and high-resolution fluorescence imaging. CONCLUSIONS: Results illustrate the potential of EGF-targeted fluorescent agents for in vivo molecular imaging, a technique that may aid in the diagnosis and characterization of oral neoplasia and allow real-time detection of tumor margins.

AB - BACKGROUND: The development of noninvasive molecular imaging approaches has the potential to improve management of cancer. METHODS: In this study, we demonstrate the potential of noninvasive topical delivery of an epidermal growth factor-Alexa 647 (EGF-Alexa 647) conjugate to image changes in epidermal growth factor receptor expression associated with oral neoplasia. We report a series of preclinical analyses to evaluate the optical contrast achieved after topical delivery of EGF-Alexa 647 in a variety of model systems, including cells, three-dimensional tissue cultures, and intact human tissue specimens using wide-field and high-resolution fluorescence imaging. Data were collected from 17 different oral cancer patients: eight pairs of normal and abnormal biopsies and nine resected tumors were examined. RESULTS: The EGF-dye conjugate can be uniformly delivered throughout the oral epithelium with a penetration depth exceeding 500 μm and incubation time of less than 30 minutes. After EGF-Alexa 647 incubation, the presence of oral neoplasia is associated with a 1.5- to 6.9-fold increase in fluorescence contrast compared with grossly normal mucosa from the same patient with both wide-field and high-resolution fluorescence imaging. CONCLUSIONS: Results illustrate the potential of EGF-targeted fluorescent agents for in vivo molecular imaging, a technique that may aid in the diagnosis and characterization of oral neoplasia and allow real-time detection of tumor margins.

UR - http://www.scopus.com/inward/record.url?scp=66449088610&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=66449088610&partnerID=8YFLogxK

U2 - 10.1593/neo.09188

DO - 10.1593/neo.09188

M3 - Article

VL - 11

SP - 542

EP - 551

JO - Neoplasia (United States)

JF - Neoplasia (United States)

SN - 1522-8002

IS - 6

ER -