Sarcoplasmic reticulum (SR) Ca2+ handling plays a key role in normal excitation-contraction coupling. This chapter briefly describes the methodology for dual optical mapping of transmembrane potential (Vm) and free intra-SR [CA2+] ([CA2+]SR) in the Langendorff-perfused rabbit heart as well as reports on novel physiological and pathophysiological insights that have been gained using this approach. Perhaps one of the most important uses of the approach is for the investigation of pathological changes to SR Ca2+ handling with disease and the circumstances in which alterations in SR Ca2+ handling may contribute to arrhythmogenesis, including focal activity, alternans, and fibrillation. Through careful measurement of SR Ca2+ content during alternans, Diaz and colleagues reported that beat-to-beat alternation of diastolic SR Ca2+ load was responsible for alternation in the amount of SR Ca2+ release. SR Ca2+ may contribute to alternation of diastolic.
- Langendorff-perfused rabbit heart
- Optical mapping
- Sarcoplasmic reticulum Ca
- Transmembrane potential
ASJC Scopus subject areas