Abstract
Purpose: The purpose of this study was to assess the feasibility of inflammation detection in an antigen-induced arthritis model using fluorescent leukocytes and optical imaging. Methods: Antigen-mediated monoarthritis was induced in the right knee of 12 Sprague-Dawley rats. Six rats remained untreated and six rats were treated with cortisone. All rats received ex vivo fluorescent-labeled rat leukocytes. Optical images of both knees were acquired before and at 5 min, 1 h, 4 h, and 24 h after cell injection. Images were evaluated qualitatively and quantitatively by calculating signal intensity ratios between the right arthritic (A) and contralateral normal (N) knee. A/N ratios were tested for significant differences between baseline values and values after cell injection using a paired t test as well as between the untreated and cortisone-treated group using an unpaired t test. Synovial specimens were processed and evaluated for labeled cells with fluorescence microscopy. Results: At 4 h and 24 h p.i., the A/N ratios of untreated arthritic knees showed a significant signal increase compared with baseline values (p<0.05) and a significant difference compared with A/N ratios of cortisone-treated animals (p<0.05). Fluorescent microscopy confirmed the presence of labeled cells in the arthritic synovium. Conclusion: Inflammation in antigen-induced arthritis can be detected with ex vivo labeled allogenic leukocytes and optical imaging.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 998-1006 |
| Number of pages | 9 |
| Journal | European Journal of Nuclear Medicine and Molecular Imaging |
| Volume | 33 |
| Issue number | 9 |
| DOIs | |
| State | Published - Sep 2006 |
| Externally published | Yes |
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Keywords
- Arthritis
- Cell tracking
- Fluorescence
- In vivo
- Optical imaging
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging
- Radiological and Ultrasound Technology
Cite this
Optical imaging of experimental arthritis using allogeneic leukocytes labeled with a near-infrared fluorescent probe. / Simon, Gerhard H.; Daldrup-Link, Heike E.; Kau, Juli; Metz, Stephan; Schlegel, Juergen; Piontek, Guido; Saborowski, Olaf; Demos, Stavros; Duyster, Justus; Pichler, Bernd J.
In: European Journal of Nuclear Medicine and Molecular Imaging, Vol. 33, No. 9, 09.2006, p. 998-1006.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Optical imaging of experimental arthritis using allogeneic leukocytes labeled with a near-infrared fluorescent probe
AU - Simon, Gerhard H.
AU - Daldrup-Link, Heike E.
AU - Kau, Juli
AU - Metz, Stephan
AU - Schlegel, Juergen
AU - Piontek, Guido
AU - Saborowski, Olaf
AU - Demos, Stavros
AU - Duyster, Justus
AU - Pichler, Bernd J.
PY - 2006/9
Y1 - 2006/9
N2 - Purpose: The purpose of this study was to assess the feasibility of inflammation detection in an antigen-induced arthritis model using fluorescent leukocytes and optical imaging. Methods: Antigen-mediated monoarthritis was induced in the right knee of 12 Sprague-Dawley rats. Six rats remained untreated and six rats were treated with cortisone. All rats received ex vivo fluorescent-labeled rat leukocytes. Optical images of both knees were acquired before and at 5 min, 1 h, 4 h, and 24 h after cell injection. Images were evaluated qualitatively and quantitatively by calculating signal intensity ratios between the right arthritic (A) and contralateral normal (N) knee. A/N ratios were tested for significant differences between baseline values and values after cell injection using a paired t test as well as between the untreated and cortisone-treated group using an unpaired t test. Synovial specimens were processed and evaluated for labeled cells with fluorescence microscopy. Results: At 4 h and 24 h p.i., the A/N ratios of untreated arthritic knees showed a significant signal increase compared with baseline values (p<0.05) and a significant difference compared with A/N ratios of cortisone-treated animals (p<0.05). Fluorescent microscopy confirmed the presence of labeled cells in the arthritic synovium. Conclusion: Inflammation in antigen-induced arthritis can be detected with ex vivo labeled allogenic leukocytes and optical imaging.
AB - Purpose: The purpose of this study was to assess the feasibility of inflammation detection in an antigen-induced arthritis model using fluorescent leukocytes and optical imaging. Methods: Antigen-mediated monoarthritis was induced in the right knee of 12 Sprague-Dawley rats. Six rats remained untreated and six rats were treated with cortisone. All rats received ex vivo fluorescent-labeled rat leukocytes. Optical images of both knees were acquired before and at 5 min, 1 h, 4 h, and 24 h after cell injection. Images were evaluated qualitatively and quantitatively by calculating signal intensity ratios between the right arthritic (A) and contralateral normal (N) knee. A/N ratios were tested for significant differences between baseline values and values after cell injection using a paired t test as well as between the untreated and cortisone-treated group using an unpaired t test. Synovial specimens were processed and evaluated for labeled cells with fluorescence microscopy. Results: At 4 h and 24 h p.i., the A/N ratios of untreated arthritic knees showed a significant signal increase compared with baseline values (p<0.05) and a significant difference compared with A/N ratios of cortisone-treated animals (p<0.05). Fluorescent microscopy confirmed the presence of labeled cells in the arthritic synovium. Conclusion: Inflammation in antigen-induced arthritis can be detected with ex vivo labeled allogenic leukocytes and optical imaging.
KW - Arthritis
KW - Cell tracking
KW - Fluorescence
KW - In vivo
KW - Optical imaging
UR - http://www.scopus.com/inward/record.url?scp=33747030901&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33747030901&partnerID=8YFLogxK
U2 - 10.1007/s00259-006-0081-y
DO - 10.1007/s00259-006-0081-y
M3 - Article
C2 - 16770602
AN - SCOPUS:33747030901
VL - 33
SP - 998
EP - 1006
JO - European Journal of Nuclear Medicine and Molecular Imaging
JF - European Journal of Nuclear Medicine and Molecular Imaging
SN - 1619-7070
IS - 9
ER -