Optical imaging of experimental arthritis using allogeneic leukocytes labeled with a near-infrared fluorescent probe

Gerhard H. Simon, Heike E. Daldrup-Link, Juli Kau, Stephan Metz, Juergen Schlegel, Guido Piontek, Olaf Saborowski, Stavros Demos, Justus Duyster, Bernd J. Pichler

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Purpose: The purpose of this study was to assess the feasibility of inflammation detection in an antigen-induced arthritis model using fluorescent leukocytes and optical imaging. Methods: Antigen-mediated monoarthritis was induced in the right knee of 12 Sprague-Dawley rats. Six rats remained untreated and six rats were treated with cortisone. All rats received ex vivo fluorescent-labeled rat leukocytes. Optical images of both knees were acquired before and at 5 min, 1 h, 4 h, and 24 h after cell injection. Images were evaluated qualitatively and quantitatively by calculating signal intensity ratios between the right arthritic (A) and contralateral normal (N) knee. A/N ratios were tested for significant differences between baseline values and values after cell injection using a paired t test as well as between the untreated and cortisone-treated group using an unpaired t test. Synovial specimens were processed and evaluated for labeled cells with fluorescence microscopy. Results: At 4 h and 24 h p.i., the A/N ratios of untreated arthritic knees showed a significant signal increase compared with baseline values (p<0.05) and a significant difference compared with A/N ratios of cortisone-treated animals (p<0.05). Fluorescent microscopy confirmed the presence of labeled cells in the arthritic synovium. Conclusion: Inflammation in antigen-induced arthritis can be detected with ex vivo labeled allogenic leukocytes and optical imaging.

Original languageEnglish (US)
Pages (from-to)998-1006
Number of pages9
JournalEuropean Journal of Nuclear Medicine and Molecular Imaging
Volume33
Issue number9
DOIs
StatePublished - Sep 2006
Externally publishedYes

Fingerprint

Experimental Arthritis
Optical Imaging
Fluorescent Dyes
Arthritis
Leukocytes
Cortisone
Knee
Antigens
Inflammation
Injections
Synovial Membrane
Fluorescence Microscopy
Sprague Dawley Rats
Microscopy

Keywords

  • Arthritis
  • Cell tracking
  • Fluorescence
  • In vivo
  • Optical imaging

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Optical imaging of experimental arthritis using allogeneic leukocytes labeled with a near-infrared fluorescent probe. / Simon, Gerhard H.; Daldrup-Link, Heike E.; Kau, Juli; Metz, Stephan; Schlegel, Juergen; Piontek, Guido; Saborowski, Olaf; Demos, Stavros; Duyster, Justus; Pichler, Bernd J.

In: European Journal of Nuclear Medicine and Molecular Imaging, Vol. 33, No. 9, 09.2006, p. 998-1006.

Research output: Contribution to journalArticle

Simon, GH, Daldrup-Link, HE, Kau, J, Metz, S, Schlegel, J, Piontek, G, Saborowski, O, Demos, S, Duyster, J & Pichler, BJ 2006, 'Optical imaging of experimental arthritis using allogeneic leukocytes labeled with a near-infrared fluorescent probe', European Journal of Nuclear Medicine and Molecular Imaging, vol. 33, no. 9, pp. 998-1006. https://doi.org/10.1007/s00259-006-0081-y
Simon, Gerhard H. ; Daldrup-Link, Heike E. ; Kau, Juli ; Metz, Stephan ; Schlegel, Juergen ; Piontek, Guido ; Saborowski, Olaf ; Demos, Stavros ; Duyster, Justus ; Pichler, Bernd J. / Optical imaging of experimental arthritis using allogeneic leukocytes labeled with a near-infrared fluorescent probe. In: European Journal of Nuclear Medicine and Molecular Imaging. 2006 ; Vol. 33, No. 9. pp. 998-1006.
@article{7fd7e1aa5099408f87f0ee17e24295c8,
title = "Optical imaging of experimental arthritis using allogeneic leukocytes labeled with a near-infrared fluorescent probe",
abstract = "Purpose: The purpose of this study was to assess the feasibility of inflammation detection in an antigen-induced arthritis model using fluorescent leukocytes and optical imaging. Methods: Antigen-mediated monoarthritis was induced in the right knee of 12 Sprague-Dawley rats. Six rats remained untreated and six rats were treated with cortisone. All rats received ex vivo fluorescent-labeled rat leukocytes. Optical images of both knees were acquired before and at 5 min, 1 h, 4 h, and 24 h after cell injection. Images were evaluated qualitatively and quantitatively by calculating signal intensity ratios between the right arthritic (A) and contralateral normal (N) knee. A/N ratios were tested for significant differences between baseline values and values after cell injection using a paired t test as well as between the untreated and cortisone-treated group using an unpaired t test. Synovial specimens were processed and evaluated for labeled cells with fluorescence microscopy. Results: At 4 h and 24 h p.i., the A/N ratios of untreated arthritic knees showed a significant signal increase compared with baseline values (p<0.05) and a significant difference compared with A/N ratios of cortisone-treated animals (p<0.05). Fluorescent microscopy confirmed the presence of labeled cells in the arthritic synovium. Conclusion: Inflammation in antigen-induced arthritis can be detected with ex vivo labeled allogenic leukocytes and optical imaging.",
keywords = "Arthritis, Cell tracking, Fluorescence, In vivo, Optical imaging",
author = "Simon, {Gerhard H.} and Daldrup-Link, {Heike E.} and Juli Kau and Stephan Metz and Juergen Schlegel and Guido Piontek and Olaf Saborowski and Stavros Demos and Justus Duyster and Pichler, {Bernd J.}",
year = "2006",
month = "9",
doi = "10.1007/s00259-006-0081-y",
language = "English (US)",
volume = "33",
pages = "998--1006",
journal = "European Journal of Nuclear Medicine and Molecular Imaging",
issn = "1619-7070",
publisher = "Springer Verlag",
number = "9",

}

TY - JOUR

T1 - Optical imaging of experimental arthritis using allogeneic leukocytes labeled with a near-infrared fluorescent probe

AU - Simon, Gerhard H.

AU - Daldrup-Link, Heike E.

AU - Kau, Juli

AU - Metz, Stephan

AU - Schlegel, Juergen

AU - Piontek, Guido

AU - Saborowski, Olaf

AU - Demos, Stavros

AU - Duyster, Justus

AU - Pichler, Bernd J.

PY - 2006/9

Y1 - 2006/9

N2 - Purpose: The purpose of this study was to assess the feasibility of inflammation detection in an antigen-induced arthritis model using fluorescent leukocytes and optical imaging. Methods: Antigen-mediated monoarthritis was induced in the right knee of 12 Sprague-Dawley rats. Six rats remained untreated and six rats were treated with cortisone. All rats received ex vivo fluorescent-labeled rat leukocytes. Optical images of both knees were acquired before and at 5 min, 1 h, 4 h, and 24 h after cell injection. Images were evaluated qualitatively and quantitatively by calculating signal intensity ratios between the right arthritic (A) and contralateral normal (N) knee. A/N ratios were tested for significant differences between baseline values and values after cell injection using a paired t test as well as between the untreated and cortisone-treated group using an unpaired t test. Synovial specimens were processed and evaluated for labeled cells with fluorescence microscopy. Results: At 4 h and 24 h p.i., the A/N ratios of untreated arthritic knees showed a significant signal increase compared with baseline values (p<0.05) and a significant difference compared with A/N ratios of cortisone-treated animals (p<0.05). Fluorescent microscopy confirmed the presence of labeled cells in the arthritic synovium. Conclusion: Inflammation in antigen-induced arthritis can be detected with ex vivo labeled allogenic leukocytes and optical imaging.

AB - Purpose: The purpose of this study was to assess the feasibility of inflammation detection in an antigen-induced arthritis model using fluorescent leukocytes and optical imaging. Methods: Antigen-mediated monoarthritis was induced in the right knee of 12 Sprague-Dawley rats. Six rats remained untreated and six rats were treated with cortisone. All rats received ex vivo fluorescent-labeled rat leukocytes. Optical images of both knees were acquired before and at 5 min, 1 h, 4 h, and 24 h after cell injection. Images were evaluated qualitatively and quantitatively by calculating signal intensity ratios between the right arthritic (A) and contralateral normal (N) knee. A/N ratios were tested for significant differences between baseline values and values after cell injection using a paired t test as well as between the untreated and cortisone-treated group using an unpaired t test. Synovial specimens were processed and evaluated for labeled cells with fluorescence microscopy. Results: At 4 h and 24 h p.i., the A/N ratios of untreated arthritic knees showed a significant signal increase compared with baseline values (p<0.05) and a significant difference compared with A/N ratios of cortisone-treated animals (p<0.05). Fluorescent microscopy confirmed the presence of labeled cells in the arthritic synovium. Conclusion: Inflammation in antigen-induced arthritis can be detected with ex vivo labeled allogenic leukocytes and optical imaging.

KW - Arthritis

KW - Cell tracking

KW - Fluorescence

KW - In vivo

KW - Optical imaging

UR - http://www.scopus.com/inward/record.url?scp=33747030901&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747030901&partnerID=8YFLogxK

U2 - 10.1007/s00259-006-0081-y

DO - 10.1007/s00259-006-0081-y

M3 - Article

C2 - 16770602

AN - SCOPUS:33747030901

VL - 33

SP - 998

EP - 1006

JO - European Journal of Nuclear Medicine and Molecular Imaging

JF - European Journal of Nuclear Medicine and Molecular Imaging

SN - 1619-7070

IS - 9

ER -