Optical fluctuation microscopy based on calculating local entropy values

Idir Yahiatène, Simon Hennig, Thomas R Huser

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

We demonstrate a novel and easy-to-use method to dramatically reduce noise and background contributions in advanced fluorescence microscopy experiments. The underlying idea is that the entropy value increases for systems with a large number of accessible energy states. Intensity fluctuations originating from photophysical or photochemical effects lead to an increased information content. Calculating the pixel-wise entropy value results in an enhancement of the signal-to-noise ratio by a factor of 90-100. Comparing ECI (entropy-based contrast-enhanced imaging) to superresolution methods such as STORM and SOFI, we find that this technique also bears substantial potential for enhancing fluctuation-based superresolution microscopies.

Original languageEnglish (US)
Pages (from-to)1-6
Number of pages6
JournalChemical Physics Letters
Volume587
DOIs
StatePublished - 2013

Keywords

  • Abbreviations
  • ECI
  • entropy-based contrast-enhanced imaging
  • pointspread function
  • PSF
  • QD
  • quantum dot
  • signal-to-noise ratio
  • SNR

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Physics and Astronomy(all)

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