Optical dopamine monitoring with dLight1 reveals mesolimbic phenotypes in a mouse model of neurofibromatosis type 1

J. Elliott Robinson, Gerard M. Coughlin, Acacia M. Hori, Jounhong Ryan Cho, Elisha D. Mackey, Zeynep Turan, Tommaso Patriarchi, Lin Tian, Viviana Gradinaru

Research output: Contribution to journalArticle

Abstract

Neurofibromatosis type 1 (NF1) is an autosomal dominant disorder whose neurodevelopmental symptoms include impaired executive function, attention, and spatial learning that could be due to perturbed mesolimbic dopaminergic circuitry. However, these circuits have never been directly assayed in vivo. We employed the genetically encoded optical dopamine sensor dLight1 to monitor dopaminergic neurotransmission in the ventral striatum of NF1 mice during motivated behavior. Additionally, we developed novel systemic AAV vectors to facilitate morphological reconstruction of dopaminergic populations in cleared tissue. We found that NF1 mice exhibit reduced spontaneous dopaminergic neurotransmission that was associated with excitation/inhibition imbalance in the ventral tegmental area and abnormal neuronal morphology. NF1 mice also had more robust dopaminergic and behavioral responses to salient visual stimuli, which were stimulus-dependent, independent of learning, and rescued by optogenetic inhibition of non-dopaminergic neurons in the VTA. Overall, these studies provide a first in vivo characterization of dopaminergic circuit function in the context of NF1 and reveal novel pathophysiological mechanisms.

Original languageEnglish (US)
Article numbere48983
JournaleLife
Volume8
DOIs
StatePublished - Sep 2019

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Neurofibromatosis 1
Dopamine
Phenotype
Networks (circuits)
Monitoring
Neurons
Synaptic Transmission
Tissue
Optogenetics
Sensors
Ventral Tegmental Area
Executive Function
Learning
Population

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Robinson, J. E., Coughlin, G. M., Hori, A. M., Cho, J. R., Mackey, E. D., Turan, Z., ... Gradinaru, V. (2019). Optical dopamine monitoring with dLight1 reveals mesolimbic phenotypes in a mouse model of neurofibromatosis type 1. eLife, 8, [e48983]. https://doi.org/10.7554/eLife.48983

Optical dopamine monitoring with dLight1 reveals mesolimbic phenotypes in a mouse model of neurofibromatosis type 1. / Robinson, J. Elliott; Coughlin, Gerard M.; Hori, Acacia M.; Cho, Jounhong Ryan; Mackey, Elisha D.; Turan, Zeynep; Patriarchi, Tommaso; Tian, Lin; Gradinaru, Viviana.

In: eLife, Vol. 8, e48983, 09.2019.

Research output: Contribution to journalArticle

Robinson, JE, Coughlin, GM, Hori, AM, Cho, JR, Mackey, ED, Turan, Z, Patriarchi, T, Tian, L & Gradinaru, V 2019, 'Optical dopamine monitoring with dLight1 reveals mesolimbic phenotypes in a mouse model of neurofibromatosis type 1', eLife, vol. 8, e48983. https://doi.org/10.7554/eLife.48983
Robinson, J. Elliott ; Coughlin, Gerard M. ; Hori, Acacia M. ; Cho, Jounhong Ryan ; Mackey, Elisha D. ; Turan, Zeynep ; Patriarchi, Tommaso ; Tian, Lin ; Gradinaru, Viviana. / Optical dopamine monitoring with dLight1 reveals mesolimbic phenotypes in a mouse model of neurofibromatosis type 1. In: eLife. 2019 ; Vol. 8.
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