TY - JOUR
T1 - Optical coherence tractography using intrinsic contrast
AU - Goergen, Craig J.
AU - Radhakrishnan, Harsha
AU - Sakadžix́, Sava
AU - Mandeville, Emiri T.
AU - Lo, Eng H.
AU - Sosnovik, David E.
AU - Srinivasan, Vivek
PY - 2012/9/15
Y1 - 2012/9/15
N2 - Organs such as the heart and brain possess intricate fiber structures that are best characterized with threedimensional imaging. For instance, diffusion-based, magnetic resonance tractography (MRT) enables studies of connectivity and remodeling during development and disease macroscopically on the millimeter scale. Here we present complementary, high-resolution microscopic optical coherence imaging and analysis methods that, when used in conjunction with clearing techniques, can characterize fiber architecture in intact organs at tissue depths exceeding 1 mm. We anticipate that these techniques can be used to study fiber architecture in situ at microscopic scales not currently accessible to diffusion magentic resonance (MR), and thus, to validate and complement macroscopic structural imaging techniques. Moreover, as these techniques use intrinsic signals and do not require tissue slicing and staining, they can be used for high-throughput, nondestructive evaluation of fiber architecture across large tissue volumes.
AB - Organs such as the heart and brain possess intricate fiber structures that are best characterized with threedimensional imaging. For instance, diffusion-based, magnetic resonance tractography (MRT) enables studies of connectivity and remodeling during development and disease macroscopically on the millimeter scale. Here we present complementary, high-resolution microscopic optical coherence imaging and analysis methods that, when used in conjunction with clearing techniques, can characterize fiber architecture in intact organs at tissue depths exceeding 1 mm. We anticipate that these techniques can be used to study fiber architecture in situ at microscopic scales not currently accessible to diffusion magentic resonance (MR), and thus, to validate and complement macroscopic structural imaging techniques. Moreover, as these techniques use intrinsic signals and do not require tissue slicing and staining, they can be used for high-throughput, nondestructive evaluation of fiber architecture across large tissue volumes.
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U2 - 10.1364/OL.37.003882
DO - 10.1364/OL.37.003882
M3 - Article
C2 - 23041891
AN - SCOPUS:84866284109
VL - 37
SP - 3882
EP - 3884
JO - Optics Letters
JF - Optics Letters
SN - 0146-9592
IS - 18
ER -