Optical coherence tomography scan circle location and mean retinal nerve fiber layer measurement variability

Michelle L. Gabriele, Hiroshi Ishikawa, Gadi Wollstein, Richard A. Bilonick, Kelly A. Townsend, Larry Kagemann, Maciej Wojtkowski, Vivek Srinivasan, James G. Fujimoto, Jay S. Duker, Joel S. Schuman

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

PURPOSE. To investigate the effect on optical coherence tomography (OCT) retinal nerve fiber layer (RNFL) thickness measurements of varying the standard 3.4-mm-diameter circle location. METHODS. The optic nerve head (ONH) region of 17 eyes of 17 healthy subjects was imaged with high-speed, ultrahigh-resolution OCT (hsUHR-OCT; 501 x 180 axial scans covering a 6 x 6-mm area; scan time, 3.84 seconds) for a comprehensive sampling. This method allows for systematic simulation of the variable circle placement effect. RNFL thickness was measured on this three-dimensional dataset by using a custom-designed software program. RNFL thickness was resampled along a 3.4-mm-diameter circle centered on the ONH, then along 3.4-mm circles shifted horizontally (x-shift), vertically (y-shift) and diagonally up to ±500 μm (at 100-μm intervals). Linear mixed-effects models were used to determine RNFL thickness as a function of the scan circle shift. A model for the distance between the two thickest measurements along the RNFL thickness circular profile (peak distance) was also calculated. RESULTS. RNFL thickness tended to decrease with both positive and negative x-and y-shifts. The range of shifts that caused a decrease greater than the variability inherent to the commercial device was greater in both nasal and temporal quadrants than in the superior and inferior ones. The model for peak distance demonstrated that as the scan moves nasally, the RNFL peak distance increases, and as the circle moves temporally, the distance decreases. Vertical shifts had a minimal effect on peak distance. CONCLUSIONS. The location of the OCT scan circle affects RNFL thickness measurements. Accurate registration of OCT scans is essential for measurement reproducibility and longitudinal examination (ClinicalTrials.gov number, NCT00286637).

Original languageEnglish (US)
Pages (from-to)2315-2321
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume49
Issue number6
DOIs
StatePublished - Jun 1 2008
Externally publishedYes

Fingerprint

Optical Coherence Tomography
Nerve Fibers
Optic Disk
Nose
Healthy Volunteers
Software
Equipment and Supplies

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Gabriele, M. L., Ishikawa, H., Wollstein, G., Bilonick, R. A., Townsend, K. A., Kagemann, L., ... Schuman, J. S. (2008). Optical coherence tomography scan circle location and mean retinal nerve fiber layer measurement variability. Investigative Ophthalmology and Visual Science, 49(6), 2315-2321. https://doi.org/10.1167/iovs.07-0873

Optical coherence tomography scan circle location and mean retinal nerve fiber layer measurement variability. / Gabriele, Michelle L.; Ishikawa, Hiroshi; Wollstein, Gadi; Bilonick, Richard A.; Townsend, Kelly A.; Kagemann, Larry; Wojtkowski, Maciej; Srinivasan, Vivek; Fujimoto, James G.; Duker, Jay S.; Schuman, Joel S.

In: Investigative Ophthalmology and Visual Science, Vol. 49, No. 6, 01.06.2008, p. 2315-2321.

Research output: Contribution to journalArticle

Gabriele, ML, Ishikawa, H, Wollstein, G, Bilonick, RA, Townsend, KA, Kagemann, L, Wojtkowski, M, Srinivasan, V, Fujimoto, JG, Duker, JS & Schuman, JS 2008, 'Optical coherence tomography scan circle location and mean retinal nerve fiber layer measurement variability', Investigative Ophthalmology and Visual Science, vol. 49, no. 6, pp. 2315-2321. https://doi.org/10.1167/iovs.07-0873
Gabriele, Michelle L. ; Ishikawa, Hiroshi ; Wollstein, Gadi ; Bilonick, Richard A. ; Townsend, Kelly A. ; Kagemann, Larry ; Wojtkowski, Maciej ; Srinivasan, Vivek ; Fujimoto, James G. ; Duker, Jay S. ; Schuman, Joel S. / Optical coherence tomography scan circle location and mean retinal nerve fiber layer measurement variability. In: Investigative Ophthalmology and Visual Science. 2008 ; Vol. 49, No. 6. pp. 2315-2321.
@article{2495fb08d24844c1a7fd095173a8fddf,
title = "Optical coherence tomography scan circle location and mean retinal nerve fiber layer measurement variability",
abstract = "PURPOSE. To investigate the effect on optical coherence tomography (OCT) retinal nerve fiber layer (RNFL) thickness measurements of varying the standard 3.4-mm-diameter circle location. METHODS. The optic nerve head (ONH) region of 17 eyes of 17 healthy subjects was imaged with high-speed, ultrahigh-resolution OCT (hsUHR-OCT; 501 x 180 axial scans covering a 6 x 6-mm area; scan time, 3.84 seconds) for a comprehensive sampling. This method allows for systematic simulation of the variable circle placement effect. RNFL thickness was measured on this three-dimensional dataset by using a custom-designed software program. RNFL thickness was resampled along a 3.4-mm-diameter circle centered on the ONH, then along 3.4-mm circles shifted horizontally (x-shift), vertically (y-shift) and diagonally up to ±500 μm (at 100-μm intervals). Linear mixed-effects models were used to determine RNFL thickness as a function of the scan circle shift. A model for the distance between the two thickest measurements along the RNFL thickness circular profile (peak distance) was also calculated. RESULTS. RNFL thickness tended to decrease with both positive and negative x-and y-shifts. The range of shifts that caused a decrease greater than the variability inherent to the commercial device was greater in both nasal and temporal quadrants than in the superior and inferior ones. The model for peak distance demonstrated that as the scan moves nasally, the RNFL peak distance increases, and as the circle moves temporally, the distance decreases. Vertical shifts had a minimal effect on peak distance. CONCLUSIONS. The location of the OCT scan circle affects RNFL thickness measurements. Accurate registration of OCT scans is essential for measurement reproducibility and longitudinal examination (ClinicalTrials.gov number, NCT00286637).",
author = "Gabriele, {Michelle L.} and Hiroshi Ishikawa and Gadi Wollstein and Bilonick, {Richard A.} and Townsend, {Kelly A.} and Larry Kagemann and Maciej Wojtkowski and Vivek Srinivasan and Fujimoto, {James G.} and Duker, {Jay S.} and Schuman, {Joel S.}",
year = "2008",
month = "6",
day = "1",
doi = "10.1167/iovs.07-0873",
language = "English (US)",
volume = "49",
pages = "2315--2321",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "6",

}

TY - JOUR

T1 - Optical coherence tomography scan circle location and mean retinal nerve fiber layer measurement variability

AU - Gabriele, Michelle L.

AU - Ishikawa, Hiroshi

AU - Wollstein, Gadi

AU - Bilonick, Richard A.

AU - Townsend, Kelly A.

AU - Kagemann, Larry

AU - Wojtkowski, Maciej

AU - Srinivasan, Vivek

AU - Fujimoto, James G.

AU - Duker, Jay S.

AU - Schuman, Joel S.

PY - 2008/6/1

Y1 - 2008/6/1

N2 - PURPOSE. To investigate the effect on optical coherence tomography (OCT) retinal nerve fiber layer (RNFL) thickness measurements of varying the standard 3.4-mm-diameter circle location. METHODS. The optic nerve head (ONH) region of 17 eyes of 17 healthy subjects was imaged with high-speed, ultrahigh-resolution OCT (hsUHR-OCT; 501 x 180 axial scans covering a 6 x 6-mm area; scan time, 3.84 seconds) for a comprehensive sampling. This method allows for systematic simulation of the variable circle placement effect. RNFL thickness was measured on this three-dimensional dataset by using a custom-designed software program. RNFL thickness was resampled along a 3.4-mm-diameter circle centered on the ONH, then along 3.4-mm circles shifted horizontally (x-shift), vertically (y-shift) and diagonally up to ±500 μm (at 100-μm intervals). Linear mixed-effects models were used to determine RNFL thickness as a function of the scan circle shift. A model for the distance between the two thickest measurements along the RNFL thickness circular profile (peak distance) was also calculated. RESULTS. RNFL thickness tended to decrease with both positive and negative x-and y-shifts. The range of shifts that caused a decrease greater than the variability inherent to the commercial device was greater in both nasal and temporal quadrants than in the superior and inferior ones. The model for peak distance demonstrated that as the scan moves nasally, the RNFL peak distance increases, and as the circle moves temporally, the distance decreases. Vertical shifts had a minimal effect on peak distance. CONCLUSIONS. The location of the OCT scan circle affects RNFL thickness measurements. Accurate registration of OCT scans is essential for measurement reproducibility and longitudinal examination (ClinicalTrials.gov number, NCT00286637).

AB - PURPOSE. To investigate the effect on optical coherence tomography (OCT) retinal nerve fiber layer (RNFL) thickness measurements of varying the standard 3.4-mm-diameter circle location. METHODS. The optic nerve head (ONH) region of 17 eyes of 17 healthy subjects was imaged with high-speed, ultrahigh-resolution OCT (hsUHR-OCT; 501 x 180 axial scans covering a 6 x 6-mm area; scan time, 3.84 seconds) for a comprehensive sampling. This method allows for systematic simulation of the variable circle placement effect. RNFL thickness was measured on this three-dimensional dataset by using a custom-designed software program. RNFL thickness was resampled along a 3.4-mm-diameter circle centered on the ONH, then along 3.4-mm circles shifted horizontally (x-shift), vertically (y-shift) and diagonally up to ±500 μm (at 100-μm intervals). Linear mixed-effects models were used to determine RNFL thickness as a function of the scan circle shift. A model for the distance between the two thickest measurements along the RNFL thickness circular profile (peak distance) was also calculated. RESULTS. RNFL thickness tended to decrease with both positive and negative x-and y-shifts. The range of shifts that caused a decrease greater than the variability inherent to the commercial device was greater in both nasal and temporal quadrants than in the superior and inferior ones. The model for peak distance demonstrated that as the scan moves nasally, the RNFL peak distance increases, and as the circle moves temporally, the distance decreases. Vertical shifts had a minimal effect on peak distance. CONCLUSIONS. The location of the OCT scan circle affects RNFL thickness measurements. Accurate registration of OCT scans is essential for measurement reproducibility and longitudinal examination (ClinicalTrials.gov number, NCT00286637).

UR - http://www.scopus.com/inward/record.url?scp=47249107711&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=47249107711&partnerID=8YFLogxK

U2 - 10.1167/iovs.07-0873

DO - 10.1167/iovs.07-0873

M3 - Article

C2 - 18515577

AN - SCOPUS:47249107711

VL - 49

SP - 2315

EP - 2321

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 6

ER -