Optical coherence tomography and Raman spectroscopy of the ex-vivo retina

Julia W. Evans, Robert Zawadzki, Rui Liu, James W Chan, Stephen M. Lane, John S Werner

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

Imaging the structure and correlating it with the biochemical content of the retina holds promise for fundamental research and for clinical applications. Optical coherence tomography (OCT) is commonly used to image the 3D structure of the retina and while the added functionality of biochemical analysis afforded by Raman scattering could provide critical molecular signatures for clinicians and researchers, there are many technical challenges to combine these imaging modalities. We describe an OCT microscope for ex-vivo imaging combined with Raman spectroscopy capable of collecting morphological and molecular information about a sample simultaneously. We present our first results and discuss the challenges to further development of this dual-mode instrument and limitations for future in-vivo retinal imaging.

Original languageEnglish (US)
Pages (from-to)398-406
Number of pages9
JournalJournal of Biophotonics
Volume2
Issue number6-7
DOIs
StatePublished - 2009

Keywords

  • Optical coherence tomography
  • Raman spectroscopy
  • Retinal imaging

ASJC Scopus subject areas

  • Materials Science(all)
  • Engineering(all)
  • Physics and Astronomy(all)
  • Chemistry(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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