Opioids suppress chemokine-mediated migration of monkey neutrophils and monocytes - An instant response

Tomoko Miyagi, Linda F. Chuang, Kenneth M. Lam, Hsiang Fu Kung, Jing Ming Wang, Bennie Osburn, Ronald Y. Chuang

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Opioid users having acquired human immunodeficiency syndrome (AIDS) are at a greater risk than non-users of contracting opportunistic infections. Opioid-administered and simian immunodeficiency virus (SIV)-infected rhesus monkeys have been an excellent model for studying AIDS and drug abuse in humans. In this study, chemotaxis of monkey leukocytes was evaluated using the chemokines interleukin-8 (IL-8) and regulated upon activation, normal T cell expressed (RANTES) as the chemoattractants, and the effects of various opioid agonists and antagonists on the efficiency of chemotaxis were examined. Opioids were either incubated with monkey leukocytes or added directly to chemokines, and the number of cells migrating toward IL-8 (for neutrophils) or RANTES (for monocytes) was scored. Inhibition of chemotaxis was seen with both assay conditions, and the inhibition was mediated by opioids binding to mu or kappa receptors. Binding to delta opiod receptors was rarely, if ever, observed. Although opioids themselves may act as weak chemoattractants for monkey leukocytes, addition of opioid agonists to chemokines would reduce the chemoattractant ability of the chemokines. Opioids did not cause the same inhibitory effect on the chemotactic migration of neutrophils when the complement component C5a or the chemotactic peptide N-formyl-MET-LEU-PHE (fMLP) was used as chemoattractant. These studies suggest that the presence of opioids during SIV infection immediately alters chemokine-mediated immune functions. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)53-62
Number of pages10
JournalImmunopharmacology
Volume47
Issue number1
DOIs
StatePublished - Apr 1 2000

Fingerprint

Chemokines
Opioid Analgesics
Haplorhini
Monocytes
Neutrophils
Chemotactic Factors
Simian Immunodeficiency Virus
Interleukin-8
Acquired Immunodeficiency Syndrome
Leukocytes
Complement C5a
Leukocyte Chemotaxis
methionyl-leucyl-phenylalanine
Cell Migration Inhibition
T-Lymphocytes
kappa Opioid Receptor
delta Opioid Receptor
Aptitude
Narcotic Antagonists
mu Opioid Receptor

Keywords

  • Chemotaxis
  • Interleukin-8 (IL-8)
  • Monkey leukocytes
  • Opioids
  • RANTES

ASJC Scopus subject areas

  • Pharmacology

Cite this

Opioids suppress chemokine-mediated migration of monkey neutrophils and monocytes - An instant response. / Miyagi, Tomoko; Chuang, Linda F.; Lam, Kenneth M.; Kung, Hsiang Fu; Wang, Jing Ming; Osburn, Bennie; Chuang, Ronald Y.

In: Immunopharmacology, Vol. 47, No. 1, 01.04.2000, p. 53-62.

Research output: Contribution to journalArticle

Miyagi, Tomoko ; Chuang, Linda F. ; Lam, Kenneth M. ; Kung, Hsiang Fu ; Wang, Jing Ming ; Osburn, Bennie ; Chuang, Ronald Y. / Opioids suppress chemokine-mediated migration of monkey neutrophils and monocytes - An instant response. In: Immunopharmacology. 2000 ; Vol. 47, No. 1. pp. 53-62.
@article{ec81c3282cdd422395559d3f82f4aaa8,
title = "Opioids suppress chemokine-mediated migration of monkey neutrophils and monocytes - An instant response",
abstract = "Opioid users having acquired human immunodeficiency syndrome (AIDS) are at a greater risk than non-users of contracting opportunistic infections. Opioid-administered and simian immunodeficiency virus (SIV)-infected rhesus monkeys have been an excellent model for studying AIDS and drug abuse in humans. In this study, chemotaxis of monkey leukocytes was evaluated using the chemokines interleukin-8 (IL-8) and regulated upon activation, normal T cell expressed (RANTES) as the chemoattractants, and the effects of various opioid agonists and antagonists on the efficiency of chemotaxis were examined. Opioids were either incubated with monkey leukocytes or added directly to chemokines, and the number of cells migrating toward IL-8 (for neutrophils) or RANTES (for monocytes) was scored. Inhibition of chemotaxis was seen with both assay conditions, and the inhibition was mediated by opioids binding to mu or kappa receptors. Binding to delta opiod receptors was rarely, if ever, observed. Although opioids themselves may act as weak chemoattractants for monkey leukocytes, addition of opioid agonists to chemokines would reduce the chemoattractant ability of the chemokines. Opioids did not cause the same inhibitory effect on the chemotactic migration of neutrophils when the complement component C5a or the chemotactic peptide N-formyl-MET-LEU-PHE (fMLP) was used as chemoattractant. These studies suggest that the presence of opioids during SIV infection immediately alters chemokine-mediated immune functions. Copyright (C) 2000 Elsevier Science B.V.",
keywords = "Chemotaxis, Interleukin-8 (IL-8), Monkey leukocytes, Opioids, RANTES",
author = "Tomoko Miyagi and Chuang, {Linda F.} and Lam, {Kenneth M.} and Kung, {Hsiang Fu} and Wang, {Jing Ming} and Bennie Osburn and Chuang, {Ronald Y.}",
year = "2000",
month = "4",
day = "1",
doi = "10.1016/S0162-3109(99)00188-5",
language = "English (US)",
volume = "47",
pages = "53--62",
journal = "Immunopharmacology",
issn = "0162-3109",
publisher = "Elsevier BV",
number = "1",

}

TY - JOUR

T1 - Opioids suppress chemokine-mediated migration of monkey neutrophils and monocytes - An instant response

AU - Miyagi, Tomoko

AU - Chuang, Linda F.

AU - Lam, Kenneth M.

AU - Kung, Hsiang Fu

AU - Wang, Jing Ming

AU - Osburn, Bennie

AU - Chuang, Ronald Y.

PY - 2000/4/1

Y1 - 2000/4/1

N2 - Opioid users having acquired human immunodeficiency syndrome (AIDS) are at a greater risk than non-users of contracting opportunistic infections. Opioid-administered and simian immunodeficiency virus (SIV)-infected rhesus monkeys have been an excellent model for studying AIDS and drug abuse in humans. In this study, chemotaxis of monkey leukocytes was evaluated using the chemokines interleukin-8 (IL-8) and regulated upon activation, normal T cell expressed (RANTES) as the chemoattractants, and the effects of various opioid agonists and antagonists on the efficiency of chemotaxis were examined. Opioids were either incubated with monkey leukocytes or added directly to chemokines, and the number of cells migrating toward IL-8 (for neutrophils) or RANTES (for monocytes) was scored. Inhibition of chemotaxis was seen with both assay conditions, and the inhibition was mediated by opioids binding to mu or kappa receptors. Binding to delta opiod receptors was rarely, if ever, observed. Although opioids themselves may act as weak chemoattractants for monkey leukocytes, addition of opioid agonists to chemokines would reduce the chemoattractant ability of the chemokines. Opioids did not cause the same inhibitory effect on the chemotactic migration of neutrophils when the complement component C5a or the chemotactic peptide N-formyl-MET-LEU-PHE (fMLP) was used as chemoattractant. These studies suggest that the presence of opioids during SIV infection immediately alters chemokine-mediated immune functions. Copyright (C) 2000 Elsevier Science B.V.

AB - Opioid users having acquired human immunodeficiency syndrome (AIDS) are at a greater risk than non-users of contracting opportunistic infections. Opioid-administered and simian immunodeficiency virus (SIV)-infected rhesus monkeys have been an excellent model for studying AIDS and drug abuse in humans. In this study, chemotaxis of monkey leukocytes was evaluated using the chemokines interleukin-8 (IL-8) and regulated upon activation, normal T cell expressed (RANTES) as the chemoattractants, and the effects of various opioid agonists and antagonists on the efficiency of chemotaxis were examined. Opioids were either incubated with monkey leukocytes or added directly to chemokines, and the number of cells migrating toward IL-8 (for neutrophils) or RANTES (for monocytes) was scored. Inhibition of chemotaxis was seen with both assay conditions, and the inhibition was mediated by opioids binding to mu or kappa receptors. Binding to delta opiod receptors was rarely, if ever, observed. Although opioids themselves may act as weak chemoattractants for monkey leukocytes, addition of opioid agonists to chemokines would reduce the chemoattractant ability of the chemokines. Opioids did not cause the same inhibitory effect on the chemotactic migration of neutrophils when the complement component C5a or the chemotactic peptide N-formyl-MET-LEU-PHE (fMLP) was used as chemoattractant. These studies suggest that the presence of opioids during SIV infection immediately alters chemokine-mediated immune functions. Copyright (C) 2000 Elsevier Science B.V.

KW - Chemotaxis

KW - Interleukin-8 (IL-8)

KW - Monkey leukocytes

KW - Opioids

KW - RANTES

UR - http://www.scopus.com/inward/record.url?scp=0034003417&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034003417&partnerID=8YFLogxK

U2 - 10.1016/S0162-3109(99)00188-5

DO - 10.1016/S0162-3109(99)00188-5

M3 - Article

VL - 47

SP - 53

EP - 62

JO - Immunopharmacology

JF - Immunopharmacology

SN - 0162-3109

IS - 1

ER -