Opening of the blood-brain barrier by intravenous contrast media in euvolemic and dehydrated rabbits

Katsumi Hayakawa, T. M. Morris, Richard W Katzberg

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

It has been suggested that intravenous injections of hypertonic contrast media when used in computed tomography and digital subtraction angiography might raise plasma osmolality sufficiently to open the blood-brain barrier (BBB). The current investigation establishes the threshold of plasma osmolality that causes the the opening of the BBB in euvolemic and dehydrated rabbits. Euvolemic rabbits were allowed food and water ad libitum. Dehydrated rabbits received 4.0 mg/kg of furosemide intramuscularly and were deprived of water for 72 hours. Meglumine/sodium diatrizoate 76 per cent (n=28) or mannitol 20 per cent (n=12) was administrated intravenously, at a rate of 25 mmol/kg body weight/hour for 2, 3 or 4 hours. Plasma osmolality, blood iodine concentration, blood pressure, heart rate and hematocrit were assessed at regular intervals. Evans blue and 99Tcm-DTPA were used simultaneously as tracers for BBB opening. Rating of BBB opening with 99Tcm-DTPA correlated well with Evans blue staining (r=0.863, p<0.001; n=42). BBB opening was related to plasma osmolality and was similar for both contrast media and mannitol. Widespread BBB opening occurred above 400 mmol/kg while focal BBB opening occurred above 370 mmol/kg. Dehydration per se increased plasma osmolality but did not reduce the threshold for BBB opening.

Original languageEnglish (US)
Pages (from-to)439-444
Number of pages6
JournalActa Radiologica
Volume30
Issue number4
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Blood-Brain Barrier
Contrast Media
Rabbits
Osmolar Concentration
Evans Blue
Pentetic Acid
Mannitol
Diatrizoate Meglumine
Diatrizoate
Digital Subtraction Angiography
Water
Furosemide
Dehydration
Hematocrit
Intravenous Injections
Iodine
Heart Rate
Tomography
Body Weight
Staining and Labeling

Keywords

  • Blood-brain barrier
  • Dehydration
  • Intravenous contrast media
  • Plasma osmolality

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology
  • Medicine(all)
  • Radiology Nuclear Medicine and imaging

Cite this

Opening of the blood-brain barrier by intravenous contrast media in euvolemic and dehydrated rabbits. / Hayakawa, Katsumi; Morris, T. M.; Katzberg, Richard W.

In: Acta Radiologica, Vol. 30, No. 4, 1989, p. 439-444.

Research output: Contribution to journalArticle

Hayakawa, Katsumi ; Morris, T. M. ; Katzberg, Richard W. / Opening of the blood-brain barrier by intravenous contrast media in euvolemic and dehydrated rabbits. In: Acta Radiologica. 1989 ; Vol. 30, No. 4. pp. 439-444.
@article{45a41d273c19491ebfd8942e0153c0b3,
title = "Opening of the blood-brain barrier by intravenous contrast media in euvolemic and dehydrated rabbits",
abstract = "It has been suggested that intravenous injections of hypertonic contrast media when used in computed tomography and digital subtraction angiography might raise plasma osmolality sufficiently to open the blood-brain barrier (BBB). The current investigation establishes the threshold of plasma osmolality that causes the the opening of the BBB in euvolemic and dehydrated rabbits. Euvolemic rabbits were allowed food and water ad libitum. Dehydrated rabbits received 4.0 mg/kg of furosemide intramuscularly and were deprived of water for 72 hours. Meglumine/sodium diatrizoate 76 per cent (n=28) or mannitol 20 per cent (n=12) was administrated intravenously, at a rate of 25 mmol/kg body weight/hour for 2, 3 or 4 hours. Plasma osmolality, blood iodine concentration, blood pressure, heart rate and hematocrit were assessed at regular intervals. Evans blue and 99Tcm-DTPA were used simultaneously as tracers for BBB opening. Rating of BBB opening with 99Tcm-DTPA correlated well with Evans blue staining (r=0.863, p<0.001; n=42). BBB opening was related to plasma osmolality and was similar for both contrast media and mannitol. Widespread BBB opening occurred above 400 mmol/kg while focal BBB opening occurred above 370 mmol/kg. Dehydration per se increased plasma osmolality but did not reduce the threshold for BBB opening.",
keywords = "Blood-brain barrier, Dehydration, Intravenous contrast media, Plasma osmolality",
author = "Katsumi Hayakawa and Morris, {T. M.} and Katzberg, {Richard W}",
year = "1989",
doi = "10.3109/02841858909174713",
language = "English (US)",
volume = "30",
pages = "439--444",
journal = "Acta Radiologica",
issn = "0284-1851",
publisher = "SAGE Publications Ltd",
number = "4",

}

TY - JOUR

T1 - Opening of the blood-brain barrier by intravenous contrast media in euvolemic and dehydrated rabbits

AU - Hayakawa, Katsumi

AU - Morris, T. M.

AU - Katzberg, Richard W

PY - 1989

Y1 - 1989

N2 - It has been suggested that intravenous injections of hypertonic contrast media when used in computed tomography and digital subtraction angiography might raise plasma osmolality sufficiently to open the blood-brain barrier (BBB). The current investigation establishes the threshold of plasma osmolality that causes the the opening of the BBB in euvolemic and dehydrated rabbits. Euvolemic rabbits were allowed food and water ad libitum. Dehydrated rabbits received 4.0 mg/kg of furosemide intramuscularly and were deprived of water for 72 hours. Meglumine/sodium diatrizoate 76 per cent (n=28) or mannitol 20 per cent (n=12) was administrated intravenously, at a rate of 25 mmol/kg body weight/hour for 2, 3 or 4 hours. Plasma osmolality, blood iodine concentration, blood pressure, heart rate and hematocrit were assessed at regular intervals. Evans blue and 99Tcm-DTPA were used simultaneously as tracers for BBB opening. Rating of BBB opening with 99Tcm-DTPA correlated well with Evans blue staining (r=0.863, p<0.001; n=42). BBB opening was related to plasma osmolality and was similar for both contrast media and mannitol. Widespread BBB opening occurred above 400 mmol/kg while focal BBB opening occurred above 370 mmol/kg. Dehydration per se increased plasma osmolality but did not reduce the threshold for BBB opening.

AB - It has been suggested that intravenous injections of hypertonic contrast media when used in computed tomography and digital subtraction angiography might raise plasma osmolality sufficiently to open the blood-brain barrier (BBB). The current investigation establishes the threshold of plasma osmolality that causes the the opening of the BBB in euvolemic and dehydrated rabbits. Euvolemic rabbits were allowed food and water ad libitum. Dehydrated rabbits received 4.0 mg/kg of furosemide intramuscularly and were deprived of water for 72 hours. Meglumine/sodium diatrizoate 76 per cent (n=28) or mannitol 20 per cent (n=12) was administrated intravenously, at a rate of 25 mmol/kg body weight/hour for 2, 3 or 4 hours. Plasma osmolality, blood iodine concentration, blood pressure, heart rate and hematocrit were assessed at regular intervals. Evans blue and 99Tcm-DTPA were used simultaneously as tracers for BBB opening. Rating of BBB opening with 99Tcm-DTPA correlated well with Evans blue staining (r=0.863, p<0.001; n=42). BBB opening was related to plasma osmolality and was similar for both contrast media and mannitol. Widespread BBB opening occurred above 400 mmol/kg while focal BBB opening occurred above 370 mmol/kg. Dehydration per se increased plasma osmolality but did not reduce the threshold for BBB opening.

KW - Blood-brain barrier

KW - Dehydration

KW - Intravenous contrast media

KW - Plasma osmolality

UR - http://www.scopus.com/inward/record.url?scp=0024313787&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024313787&partnerID=8YFLogxK

U2 - 10.3109/02841858909174713

DO - 10.3109/02841858909174713

M3 - Article

VL - 30

SP - 439

EP - 444

JO - Acta Radiologica

JF - Acta Radiologica

SN - 0284-1851

IS - 4

ER -