Ontogeny of the v-erbA oncoprotein from the thyroid hormone receptor: An alteration in the DNA binding domain plays a role crucial for v-erbA function

Beverly G. Bonde, Mohammed Sharif, Martin L. Privalsky

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

The avian erythroblastosis virus v-erbA oncogene is imprecisely derived from a cellular gene (c-erbA) encoding a thyroid hormone receptor: the v-erbA protein has sustained both small terminal deletions and internal amino acid sequence changes relative to c-erbA. We report here that one of these missense differences between v- and c-erbA proteins, located in a zinc finger DNA binding domain, has dramatic effects on the biological activities of the encoded protein. Back mutation of the viral coding sequence to resemble c-erbA at this site severely impairs erythroid transformation and produces subtle changes in DNA binding by the encoded protein, suggesting that differences in DNA binding by the viral and cellular proteins may be involved in the activation of v-erbA as an oncogene.

Original languageEnglish (US)
Pages (from-to)2037-2046
Number of pages10
JournalJournal of Virology
Volume65
Issue number4
StatePublished - 1991

ASJC Scopus subject areas

  • Immunology

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