Ontogeny of the common carp (Cyprinus carpio L.) innate immune system

Heidi B T Huttenhuis, Anja J. Taverne-Thiele, Cláudia P O Grou, Jorrit Bergsma, Jeroen Saeij, Chihaya Nakayasu, Jan H W M Rombout

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

The ontogeny of the teleost innate immune system was studied in carp using cellular, histological and quantitative molecular techniques. Carp myeloid cells first appeared ventro-lateral of the aorta at 2 days post fertilization (the start of hatching), and subsequently around the sinuses of the vena cardinalis (or posterior blood islet), head kidney and trunk kidney. In addition, the hematopoietic tissue around the sinuses of the vena cardinalis transformed into that of the trunk kidney, which is the first description of the ontogeny of the trunk kidney hematopoietic tissue in teleosts. The mAb's used in this study reacted with carp myeloid surface molecules that are already transcribed and processed from the first appearance of myeloid cells, and thus serve a significant role in unraveling ontogenetic processes of teleost immunology. Finally, this study associated the first appearance of myeloid cells with an immune response on the molecular level: 2 days post fertilization embryos responded to LPS injection with upregulation of interleukin 1-β, inducible nitric oxide synthase and serum amyloid A, and down-regulation of complement factor 3 and alpha2-macroglobulin, implying a functional embryonic innate defense system.

Original languageEnglish (US)
Pages (from-to)557-574
Number of pages18
JournalDevelopmental and Comparative Immunology
Volume30
Issue number6
DOIs
StatePublished - 2006
Externally publishedYes

Fingerprint

Carps
Myeloid Cells
Immune System
Kidney
Fertilization
Head Kidney
alpha-Macroglobulins
Serum Amyloid A Protein
Complement C3
Nitric Oxide Synthase Type II
Allergy and Immunology
Interleukin-1
Aorta
Up-Regulation
Down-Regulation
Embryonic Structures
Injections

Keywords

  • Acute phase response
  • Antibodies
  • Cytokines
  • Development
  • Fish
  • Immune response
  • Innate immune system
  • Myelopoiesis

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

Cite this

Huttenhuis, H. B. T., Taverne-Thiele, A. J., Grou, C. P. O., Bergsma, J., Saeij, J., Nakayasu, C., & Rombout, J. H. W. M. (2006). Ontogeny of the common carp (Cyprinus carpio L.) innate immune system. Developmental and Comparative Immunology, 30(6), 557-574. https://doi.org/10.1016/j.dci.2005.08.001

Ontogeny of the common carp (Cyprinus carpio L.) innate immune system. / Huttenhuis, Heidi B T; Taverne-Thiele, Anja J.; Grou, Cláudia P O; Bergsma, Jorrit; Saeij, Jeroen; Nakayasu, Chihaya; Rombout, Jan H W M.

In: Developmental and Comparative Immunology, Vol. 30, No. 6, 2006, p. 557-574.

Research output: Contribution to journalArticle

Huttenhuis, HBT, Taverne-Thiele, AJ, Grou, CPO, Bergsma, J, Saeij, J, Nakayasu, C & Rombout, JHWM 2006, 'Ontogeny of the common carp (Cyprinus carpio L.) innate immune system', Developmental and Comparative Immunology, vol. 30, no. 6, pp. 557-574. https://doi.org/10.1016/j.dci.2005.08.001
Huttenhuis, Heidi B T ; Taverne-Thiele, Anja J. ; Grou, Cláudia P O ; Bergsma, Jorrit ; Saeij, Jeroen ; Nakayasu, Chihaya ; Rombout, Jan H W M. / Ontogeny of the common carp (Cyprinus carpio L.) innate immune system. In: Developmental and Comparative Immunology. 2006 ; Vol. 30, No. 6. pp. 557-574.
@article{7e0ed8ba9e9042ee87a504778d3d91c8,
title = "Ontogeny of the common carp (Cyprinus carpio L.) innate immune system",
abstract = "The ontogeny of the teleost innate immune system was studied in carp using cellular, histological and quantitative molecular techniques. Carp myeloid cells first appeared ventro-lateral of the aorta at 2 days post fertilization (the start of hatching), and subsequently around the sinuses of the vena cardinalis (or posterior blood islet), head kidney and trunk kidney. In addition, the hematopoietic tissue around the sinuses of the vena cardinalis transformed into that of the trunk kidney, which is the first description of the ontogeny of the trunk kidney hematopoietic tissue in teleosts. The mAb's used in this study reacted with carp myeloid surface molecules that are already transcribed and processed from the first appearance of myeloid cells, and thus serve a significant role in unraveling ontogenetic processes of teleost immunology. Finally, this study associated the first appearance of myeloid cells with an immune response on the molecular level: 2 days post fertilization embryos responded to LPS injection with upregulation of interleukin 1-β, inducible nitric oxide synthase and serum amyloid A, and down-regulation of complement factor 3 and alpha2-macroglobulin, implying a functional embryonic innate defense system.",
keywords = "Acute phase response, Antibodies, Cytokines, Development, Fish, Immune response, Innate immune system, Myelopoiesis",
author = "Huttenhuis, {Heidi B T} and Taverne-Thiele, {Anja J.} and Grou, {Cl{\'a}udia P O} and Jorrit Bergsma and Jeroen Saeij and Chihaya Nakayasu and Rombout, {Jan H W M}",
year = "2006",
doi = "10.1016/j.dci.2005.08.001",
language = "English (US)",
volume = "30",
pages = "557--574",
journal = "Developmental and Comparative Immunology",
issn = "0145-305X",
publisher = "Elsevier Limited",
number = "6",

}

TY - JOUR

T1 - Ontogeny of the common carp (Cyprinus carpio L.) innate immune system

AU - Huttenhuis, Heidi B T

AU - Taverne-Thiele, Anja J.

AU - Grou, Cláudia P O

AU - Bergsma, Jorrit

AU - Saeij, Jeroen

AU - Nakayasu, Chihaya

AU - Rombout, Jan H W M

PY - 2006

Y1 - 2006

N2 - The ontogeny of the teleost innate immune system was studied in carp using cellular, histological and quantitative molecular techniques. Carp myeloid cells first appeared ventro-lateral of the aorta at 2 days post fertilization (the start of hatching), and subsequently around the sinuses of the vena cardinalis (or posterior blood islet), head kidney and trunk kidney. In addition, the hematopoietic tissue around the sinuses of the vena cardinalis transformed into that of the trunk kidney, which is the first description of the ontogeny of the trunk kidney hematopoietic tissue in teleosts. The mAb's used in this study reacted with carp myeloid surface molecules that are already transcribed and processed from the first appearance of myeloid cells, and thus serve a significant role in unraveling ontogenetic processes of teleost immunology. Finally, this study associated the first appearance of myeloid cells with an immune response on the molecular level: 2 days post fertilization embryos responded to LPS injection with upregulation of interleukin 1-β, inducible nitric oxide synthase and serum amyloid A, and down-regulation of complement factor 3 and alpha2-macroglobulin, implying a functional embryonic innate defense system.

AB - The ontogeny of the teleost innate immune system was studied in carp using cellular, histological and quantitative molecular techniques. Carp myeloid cells first appeared ventro-lateral of the aorta at 2 days post fertilization (the start of hatching), and subsequently around the sinuses of the vena cardinalis (or posterior blood islet), head kidney and trunk kidney. In addition, the hematopoietic tissue around the sinuses of the vena cardinalis transformed into that of the trunk kidney, which is the first description of the ontogeny of the trunk kidney hematopoietic tissue in teleosts. The mAb's used in this study reacted with carp myeloid surface molecules that are already transcribed and processed from the first appearance of myeloid cells, and thus serve a significant role in unraveling ontogenetic processes of teleost immunology. Finally, this study associated the first appearance of myeloid cells with an immune response on the molecular level: 2 days post fertilization embryos responded to LPS injection with upregulation of interleukin 1-β, inducible nitric oxide synthase and serum amyloid A, and down-regulation of complement factor 3 and alpha2-macroglobulin, implying a functional embryonic innate defense system.

KW - Acute phase response

KW - Antibodies

KW - Cytokines

KW - Development

KW - Fish

KW - Immune response

KW - Innate immune system

KW - Myelopoiesis

UR - http://www.scopus.com/inward/record.url?scp=33644805866&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33644805866&partnerID=8YFLogxK

U2 - 10.1016/j.dci.2005.08.001

DO - 10.1016/j.dci.2005.08.001

M3 - Article

C2 - 16406121

AN - SCOPUS:33644805866

VL - 30

SP - 557

EP - 574

JO - Developmental and Comparative Immunology

JF - Developmental and Comparative Immunology

SN - 0145-305X

IS - 6

ER -