Ontogeny of the common carp (Cyprinus carpio L.) innate immune system

Heidi B T Huttenhuis, Anja J. Taverne-Thiele, Cláudia P O Grou, Jorrit Bergsma, Jeroen Saeij, Chihaya Nakayasu, Jan H W M Rombout

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

The ontogeny of the teleost innate immune system was studied in carp using cellular, histological and quantitative molecular techniques. Carp myeloid cells first appeared ventro-lateral of the aorta at 2 days post fertilization (the start of hatching), and subsequently around the sinuses of the vena cardinalis (or posterior blood islet), head kidney and trunk kidney. In addition, the hematopoietic tissue around the sinuses of the vena cardinalis transformed into that of the trunk kidney, which is the first description of the ontogeny of the trunk kidney hematopoietic tissue in teleosts. The mAb's used in this study reacted with carp myeloid surface molecules that are already transcribed and processed from the first appearance of myeloid cells, and thus serve a significant role in unraveling ontogenetic processes of teleost immunology. Finally, this study associated the first appearance of myeloid cells with an immune response on the molecular level: 2 days post fertilization embryos responded to LPS injection with upregulation of interleukin 1-β, inducible nitric oxide synthase and serum amyloid A, and down-regulation of complement factor 3 and alpha2-macroglobulin, implying a functional embryonic innate defense system.

Original languageEnglish (US)
Pages (from-to)557-574
Number of pages18
JournalDevelopmental and Comparative Immunology
Volume30
Issue number6
DOIs
StatePublished - 2006
Externally publishedYes

Keywords

  • Acute phase response
  • Antibodies
  • Cytokines
  • Development
  • Fish
  • Immune response
  • Innate immune system
  • Myelopoiesis

ASJC Scopus subject areas

  • Developmental Biology
  • Immunology

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  • Cite this

    Huttenhuis, H. B. T., Taverne-Thiele, A. J., Grou, C. P. O., Bergsma, J., Saeij, J., Nakayasu, C., & Rombout, J. H. W. M. (2006). Ontogeny of the common carp (Cyprinus carpio L.) innate immune system. Developmental and Comparative Immunology, 30(6), 557-574. https://doi.org/10.1016/j.dci.2005.08.001