Ontogeny of atrial natriuretic peptide and its receptor in the lung: Effects on perinatal surfactant release

Christopher A. D'Angelis, Bruce A. Holm, Satyanarayana Lakshminrusimha, Peter A. Nickerson, Daniel D. Swartz, June Sokolowski, Lori C. Nielsen, Rita M. Ryan

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

During the transition at birth to air breathing, regulation of surfactant release from alveolar type II (ATII) cells is critical. Atrial natriuretic peptide (ANP) stimulates natriuretic peptide receptor-A (NPR-A) and increases intracellular cGMP. We examined the changes in ANP and NPR-A in respiratory epithelium during the perinatal period using immunohistochemistry and studied the effect of ANP on surfactant release from ATII cells isolated from fetal and newborn lambs. NPR-A mRNA was detected in the fetal lung by Northern Blot and RT-PCR. At 100 d gestation (term 145 d), ANP staining was absent and NPR-A staining was weak in cuboidal epithelial cells. ANP and NPR-A staining was prominent in ATII cells at 136 d gestation and was undetectable postnatally. ANP stimulated (maximal effect at 10M) surfactant release from both late gestation fetal and neonatal ATII cells. Protein kinase G inhibition significantly blocked this release. We conclude that ANP stimulates surfactant release in isolated perinatal ATII cells by a cGMP-dependent mechanism. ANP and NPR-A expression in ATII cells is greatest in late gestation and declines sharply postnatally. We speculate that increased activity of the ANP/NPR-A pathway in late gestation may prime the surfactant system, preparing the lung for air breathing.

Original languageEnglish (US)
Pages (from-to)239-244
Number of pages6
JournalPediatric Research
Volume63
Issue number3
DOIs
StatePublished - Mar 1 2008
Externally publishedYes

Fingerprint

Atrial Natriuretic Factor Receptors
Atrial Natriuretic Factor
Surface-Active Agents
Alveolar Epithelial Cells
Lung
Pregnancy
Staining and Labeling
Respiration
Air
Cyclic GMP-Dependent Protein Kinases
Respiratory Mucosa
atrial natriuretic factor receptor A
Northern Blotting
Epithelial Cells
Immunohistochemistry
Parturition

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Ontogeny of atrial natriuretic peptide and its receptor in the lung : Effects on perinatal surfactant release. / D'Angelis, Christopher A.; Holm, Bruce A.; Lakshminrusimha, Satyanarayana; Nickerson, Peter A.; Swartz, Daniel D.; Sokolowski, June; Nielsen, Lori C.; Ryan, Rita M.

In: Pediatric Research, Vol. 63, No. 3, 01.03.2008, p. 239-244.

Research output: Contribution to journalArticle

D'Angelis, CA, Holm, BA, Lakshminrusimha, S, Nickerson, PA, Swartz, DD, Sokolowski, J, Nielsen, LC & Ryan, RM 2008, 'Ontogeny of atrial natriuretic peptide and its receptor in the lung: Effects on perinatal surfactant release', Pediatric Research, vol. 63, no. 3, pp. 239-244. https://doi.org/10.1203/PDR.0b013e318163a215
D'Angelis, Christopher A. ; Holm, Bruce A. ; Lakshminrusimha, Satyanarayana ; Nickerson, Peter A. ; Swartz, Daniel D. ; Sokolowski, June ; Nielsen, Lori C. ; Ryan, Rita M. / Ontogeny of atrial natriuretic peptide and its receptor in the lung : Effects on perinatal surfactant release. In: Pediatric Research. 2008 ; Vol. 63, No. 3. pp. 239-244.
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