Oncogenic signaling pathways activated in DMBA-induced mouse mammary tumors

Nicolas Currier, Sandra E. Solomon, Elizabeth G. Demicco, Donny L.F. Chang, Marganit Farago, Haoqiang Ying, Isabel Dominguez, Gail E. Sonenshein, Robert Cardiff, Zhi Xiong Jim Xiao, David H. Sherr, David C. Seldin

Research output: Contribution to journalArticle

105 Scopus citations

Abstract

Only about 5% of human breast cancers can be attributed to inheritance of breast cancer susceptibility genes, while the balance are considered to be sporadic in origin. Breast cancer incidence varies with diet and other environmental influences, including carcinogen exposure. However, the effects of environmental carcinogens on cell growth control pathways are poorly understood. Here we have examined oncogenic signaling pathways that are activated in mammary tumors in mice treated with the prototypical polycyclic aromatic hydrocarbon (PAH) 7,12-dimethylbenz[a]anthracene (DMBA). In female FVB mice given 6 doses of 1 mg of DMBA by weekly gavage beginning at 5 weeks of age, all of the mice developed tumors by 34 weeks of age (median 20 weeks after beginning DMBA); 75% of the mice had mammary tumors. DMBA-induced mammary tumors exhibited elevated expression of the aryl hydrocarbon receptor (AhR), c-myc, cyclin D1, and hyperphosphorylated retinoblastoma (Rb) protein. Because of this, the activation of upstream regulatory pathways was assessed, and elements of the Wnt signaling pathway, the NF-κB pathway, and the prolyl isomerase Pin-1 were found to be frequently up-regulated in the tumors when compared to normal mammary gland controls. These data suggest that environmental carcinogens can produce long-lasting alterations in growth and anti-apoptotic pathways, leading to mammary tumorigenesis.

Original languageEnglish (US)
Pages (from-to)726-737
Number of pages12
JournalToxicologic Pathology
Volume33
Issue number6
DOIs
StatePublished - Oct 1 2005

Keywords

  • AhR
  • DMBA
  • Mammary tumorigenesis
  • NF-κb
  • Pin-1
  • Wnt

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Toxicology
  • Cell Biology

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  • Cite this

    Currier, N., Solomon, S. E., Demicco, E. G., Chang, D. L. F., Farago, M., Ying, H., Dominguez, I., Sonenshein, G. E., Cardiff, R., Xiao, Z. X. J., Sherr, D. H., & Seldin, D. C. (2005). Oncogenic signaling pathways activated in DMBA-induced mouse mammary tumors. Toxicologic Pathology, 33(6), 726-737. https://doi.org/10.1080/01926230500352226