Oncogene coexpression in mesenchymal neoplasia correlates with EGF transcription

David E. Joyner, Timothy A. Damron, Albert J. Aboulafia, R Randall

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Epidermal growth factor (EGF) is a potent mitogenic factor for cells of mesodermal and ectodermal origin, and its over-expression is associated with a variety of cancers. We asked whether oncogene coexpression occurs in mesenchymal neoplasms, if coexpression correlates with EGF transcription, and whether coexpression can be attributed to the EGF-induced overexpression of oncogenes. We quantified the mRNA concentrations of EGF and 14 oncogenes in 42 primary sarcomas, 31 benign tumors, and 10 skeletal muscle controls, and compared mRNA concentrations and gene pair correlations in EGF positive (EGF) tumors to transcript concentrations and correlations in EGF negative (EGF) tumors. Transcripts were detected by real time reverse transcription-polymerase chain reaction. Pearson's correlation coefficients identified gene associations, and gene synchrony associated with EGF expression was evaluated using chi square. Transcript concentrations in tumors were compared graphically and with t tests. Gene correlations predominated in EGF benign tumors and in EGF primary sarcomas. The dichotomy in oncogene coexpression evident in benign and malignant tumors could not be attributed to statistical differences in mRNA content between EGF and EGF tumors. EGF may enhance, or may indicate the presence of, oncogene coexpression in benign mesenchymal lesions, but counters gene synchronization in sarcomas.

Original languageEnglish (US)
Pages (from-to)14-21
Number of pages8
JournalClinical Orthopaedics and Related Research
Issue number459
DOIs
StatePublished - Jun 1 2007
Externally publishedYes

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Oncogenes
Epidermal Growth Factor
Neoplasms
Sarcoma
Genes
Messenger RNA
Reverse Transcription
Skeletal Muscle

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Surgery

Cite this

Oncogene coexpression in mesenchymal neoplasia correlates with EGF transcription. / Joyner, David E.; Damron, Timothy A.; Aboulafia, Albert J.; Randall, R.

In: Clinical Orthopaedics and Related Research, No. 459, 01.06.2007, p. 14-21.

Research output: Contribution to journalArticle

Joyner, David E. ; Damron, Timothy A. ; Aboulafia, Albert J. ; Randall, R. / Oncogene coexpression in mesenchymal neoplasia correlates with EGF transcription. In: Clinical Orthopaedics and Related Research. 2007 ; No. 459. pp. 14-21.
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