Abstract
Epidermal growth factor (EGF) is a potent mitogenic factor for cells of mesodermal and ectodermal origin, and its over-expression is associated with a variety of cancers. We asked whether oncogene coexpression occurs in mesenchymal neoplasms, if coexpression correlates with EGF transcription, and whether coexpression can be attributed to the EGF-induced overexpression of oncogenes. We quantified the mRNA concentrations of EGF and 14 oncogenes in 42 primary sarcomas, 31 benign tumors, and 10 skeletal muscle controls, and compared mRNA concentrations and gene pair correlations in EGF positive (EGF) tumors to transcript concentrations and correlations in EGF negative (EGF) tumors. Transcripts were detected by real time reverse transcription-polymerase chain reaction. Pearson's correlation coefficients identified gene associations, and gene synchrony associated with EGF expression was evaluated using chi square. Transcript concentrations in tumors were compared graphically and with t tests. Gene correlations predominated in EGF benign tumors and in EGF primary sarcomas. The dichotomy in oncogene coexpression evident in benign and malignant tumors could not be attributed to statistical differences in mRNA content between EGF and EGF tumors. EGF may enhance, or may indicate the presence of, oncogene coexpression in benign mesenchymal lesions, but counters gene synchronization in sarcomas.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 14-21 |
| Number of pages | 8 |
| Journal | Clinical Orthopaedics and Related Research |
| Issue number | 459 |
| DOIs | |
| State | Published - Jun 1 2007 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Orthopedics and Sports Medicine
- Surgery
Cite this
Oncogene coexpression in mesenchymal neoplasia correlates with EGF transcription. / Joyner, David E.; Damron, Timothy A.; Aboulafia, Albert J.; Randall, R.
In: Clinical Orthopaedics and Related Research, No. 459, 01.06.2007, p. 14-21.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oncogene coexpression in mesenchymal neoplasia correlates with EGF transcription
AU - Joyner, David E.
AU - Damron, Timothy A.
AU - Aboulafia, Albert J.
AU - Randall, R
PY - 2007/6/1
Y1 - 2007/6/1
N2 - Epidermal growth factor (EGF) is a potent mitogenic factor for cells of mesodermal and ectodermal origin, and its over-expression is associated with a variety of cancers. We asked whether oncogene coexpression occurs in mesenchymal neoplasms, if coexpression correlates with EGF transcription, and whether coexpression can be attributed to the EGF-induced overexpression of oncogenes. We quantified the mRNA concentrations of EGF and 14 oncogenes in 42 primary sarcomas, 31 benign tumors, and 10 skeletal muscle controls, and compared mRNA concentrations and gene pair correlations in EGF positive (EGF) tumors to transcript concentrations and correlations in EGF negative (EGF) tumors. Transcripts were detected by real time reverse transcription-polymerase chain reaction. Pearson's correlation coefficients identified gene associations, and gene synchrony associated with EGF expression was evaluated using chi square. Transcript concentrations in tumors were compared graphically and with t tests. Gene correlations predominated in EGF benign tumors and in EGF primary sarcomas. The dichotomy in oncogene coexpression evident in benign and malignant tumors could not be attributed to statistical differences in mRNA content between EGF and EGF tumors. EGF may enhance, or may indicate the presence of, oncogene coexpression in benign mesenchymal lesions, but counters gene synchronization in sarcomas.
AB - Epidermal growth factor (EGF) is a potent mitogenic factor for cells of mesodermal and ectodermal origin, and its over-expression is associated with a variety of cancers. We asked whether oncogene coexpression occurs in mesenchymal neoplasms, if coexpression correlates with EGF transcription, and whether coexpression can be attributed to the EGF-induced overexpression of oncogenes. We quantified the mRNA concentrations of EGF and 14 oncogenes in 42 primary sarcomas, 31 benign tumors, and 10 skeletal muscle controls, and compared mRNA concentrations and gene pair correlations in EGF positive (EGF) tumors to transcript concentrations and correlations in EGF negative (EGF) tumors. Transcripts were detected by real time reverse transcription-polymerase chain reaction. Pearson's correlation coefficients identified gene associations, and gene synchrony associated with EGF expression was evaluated using chi square. Transcript concentrations in tumors were compared graphically and with t tests. Gene correlations predominated in EGF benign tumors and in EGF primary sarcomas. The dichotomy in oncogene coexpression evident in benign and malignant tumors could not be attributed to statistical differences in mRNA content between EGF and EGF tumors. EGF may enhance, or may indicate the presence of, oncogene coexpression in benign mesenchymal lesions, but counters gene synchronization in sarcomas.
UR - http://www.scopus.com/inward/record.url?scp=34249936618&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=34249936618&partnerID=8YFLogxK
U2 - 10.1097/BLO.0b013e318059b8b1
DO - 10.1097/BLO.0b013e318059b8b1
M3 - Article
C2 - 17414167
AN - SCOPUS:34249936618
SP - 14
EP - 21
JO - Clinical Orthopaedics and Related Research
JF - Clinical Orthopaedics and Related Research
SN - 0009-921X
IS - 459
ER -