"On-the-fly" kinetics of enzymatic racemization using deuterium NMR in DNA-based chiral oriented media

Monique Chan-Huot, Philippe Lesot, Philippe Pelupessy, Luminita Duma, Geoffrey Bodenhausen, Patricia Duchambon, Michael D. Toney, U. Venkateswara Reddy, N. Suryaprakash

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We report the in situ and real-time monitoring of the interconversion of l- and d-alanine-d3 by alanine racemase from Bacillus stearothermophilus directly observed by 2H NMR spectroscopy in anisotropic phase. The enantiomers are distinguished by the difference of their 2H quadrupolar splittings in a chiral liquid crystal containing short DNA fragments. The proof-of-principle, the reliability, and the robustness of this new method is demonstrated by the determination of the turnover rates of the enzyme using the Michaelis-Menten model.

Original languageEnglish (US)
Pages (from-to)4694-4697
Number of pages4
JournalAnalytical Chemistry
Volume85
Issue number9
DOIs
StatePublished - May 7 2013

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Alanine Racemase
Liquid Crystals
Enantiomers
Deuterium
Bacilli
Alanine
Nuclear magnetic resonance spectroscopy
Nuclear magnetic resonance
Kinetics
Monitoring
DNA
Enzymes

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Chan-Huot, M., Lesot, P., Pelupessy, P., Duma, L., Bodenhausen, G., Duchambon, P., ... Suryaprakash, N. (2013). "On-the-fly" kinetics of enzymatic racemization using deuterium NMR in DNA-based chiral oriented media. Analytical Chemistry, 85(9), 4694-4697. https://doi.org/10.1021/ac4004002

"On-the-fly" kinetics of enzymatic racemization using deuterium NMR in DNA-based chiral oriented media. / Chan-Huot, Monique; Lesot, Philippe; Pelupessy, Philippe; Duma, Luminita; Bodenhausen, Geoffrey; Duchambon, Patricia; Toney, Michael D.; Reddy, U. Venkateswara; Suryaprakash, N.

In: Analytical Chemistry, Vol. 85, No. 9, 07.05.2013, p. 4694-4697.

Research output: Contribution to journalArticle

Chan-Huot, M, Lesot, P, Pelupessy, P, Duma, L, Bodenhausen, G, Duchambon, P, Toney, MD, Reddy, UV & Suryaprakash, N 2013, '"On-the-fly" kinetics of enzymatic racemization using deuterium NMR in DNA-based chiral oriented media', Analytical Chemistry, vol. 85, no. 9, pp. 4694-4697. https://doi.org/10.1021/ac4004002
Chan-Huot M, Lesot P, Pelupessy P, Duma L, Bodenhausen G, Duchambon P et al. "On-the-fly" kinetics of enzymatic racemization using deuterium NMR in DNA-based chiral oriented media. Analytical Chemistry. 2013 May 7;85(9):4694-4697. https://doi.org/10.1021/ac4004002
Chan-Huot, Monique ; Lesot, Philippe ; Pelupessy, Philippe ; Duma, Luminita ; Bodenhausen, Geoffrey ; Duchambon, Patricia ; Toney, Michael D. ; Reddy, U. Venkateswara ; Suryaprakash, N. / "On-the-fly" kinetics of enzymatic racemization using deuterium NMR in DNA-based chiral oriented media. In: Analytical Chemistry. 2013 ; Vol. 85, No. 9. pp. 4694-4697.
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