On-line detection of somatic cells in milk

Janice Wu, Michael Delwiche, James S Cullor, Wayne Smith

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Subclinical mastitis is a costly disease of the dairy industry and is presently diagnosed through somatic cell counts. Current detection methods include automated machines at remote lab sites and subjective cowside tests. An alternate method was developed to quantify somatic cells using a rapid assay and sensor that were designed to be easily amenable to an on-line somatic cell sensor. The detection was based on fluorescence from PicoGreen labeled DNA, which was extracted from cells by diluting milk 1:10 with a high pH buffer and a nonionic detergent. The high pH buffer served to reduce milk opacity and eliminate DNases, and the nonionic detergent served to lyse cells and release DNA into solution. A sensor was designed to measure fluorescence based on the dye's peak excitation of 480 nm and peak emission of 520 nm.

Original languageEnglish (US)
Title of host publicationASAE Annual International Meeting 2004
Pages7743-7760
Number of pages18
StatePublished - 2004
EventASAE Annual International Meeting 2004 - Ottawa, ON, Canada
Duration: Aug 1 2004Aug 4 2004

Other

OtherASAE Annual International Meeting 2004
CountryCanada
CityOttawa, ON
Period8/1/048/4/04

Keywords

  • DNA sensor
  • Mastitis
  • Milk
  • Somatic cell count

ASJC Scopus subject areas

  • Engineering(all)

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    Wu, J., Delwiche, M., Cullor, J. S., & Smith, W. (2004). On-line detection of somatic cells in milk. In ASAE Annual International Meeting 2004 (pp. 7743-7760)