On-enzyme refolding permits small RNA and tRNA surveillance by the CCA-adding enzyme

Claus D. Kuhn, Jeremy E. Wilusz, Yuxuan Zheng, Peter A. Beal, Leemor Joshua-Tor

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Transcription in eukaryotes produces a number of long noncoding RNAs (lncRNAs). Two of these, MALAT1 and Menβ, generate a tRNA-like small RNA in addition to the mature lncRNA. The stability of these tRNA-like small RNAs and bona fide tRNAs is monitored by the CCA-adding enzyme. Whereas CCA is added to stable tRNAs and tRNA-like transcripts, a second CCA repeat is added to certain unstable transcripts to initiate their degradation. Here, we characterize how these two scenarios are distinguished. Following the first CCA addition cycle, nucleotide binding to the active site triggers a clockwise screw motion, producing torque on the RNA. This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle. Intriguingly, with the CCA-adding enzyme acting as a molecular vise, the RNAs proofread themselves through differential responses to its interrogation between stable and unstable substrates.

Original languageEnglish (US)
Pages (from-to)644-658
Number of pages15
JournalCell
Volume160
Issue number4
DOIs
StatePublished - Feb 12 2015

Fingerprint

Transfer RNA
RNA
Long Noncoding RNA
Enzymes
RNA Folding
Torque
Eukaryota
Transcription
Catalytic Domain
Nucleotides
tRNA nucleotidyltransferase
Degradation
Substrates

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Kuhn, C. D., Wilusz, J. E., Zheng, Y., Beal, P. A., & Joshua-Tor, L. (2015). On-enzyme refolding permits small RNA and tRNA surveillance by the CCA-adding enzyme. Cell, 160(4), 644-658. https://doi.org/10.1016/j.cell.2015.01.005

On-enzyme refolding permits small RNA and tRNA surveillance by the CCA-adding enzyme. / Kuhn, Claus D.; Wilusz, Jeremy E.; Zheng, Yuxuan; Beal, Peter A.; Joshua-Tor, Leemor.

In: Cell, Vol. 160, No. 4, 12.02.2015, p. 644-658.

Research output: Contribution to journalArticle

Kuhn, CD, Wilusz, JE, Zheng, Y, Beal, PA & Joshua-Tor, L 2015, 'On-enzyme refolding permits small RNA and tRNA surveillance by the CCA-adding enzyme', Cell, vol. 160, no. 4, pp. 644-658. https://doi.org/10.1016/j.cell.2015.01.005
Kuhn, Claus D. ; Wilusz, Jeremy E. ; Zheng, Yuxuan ; Beal, Peter A. ; Joshua-Tor, Leemor. / On-enzyme refolding permits small RNA and tRNA surveillance by the CCA-adding enzyme. In: Cell. 2015 ; Vol. 160, No. 4. pp. 644-658.
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