On-demand self-assembly of supported membranes using sacrificial, anhydrobiotic sugar coats

Thomas E. Wilkop, Jeremy Sanborn, Ann E. Oliver, Joshua M. Hanson, Atul N. Parikh

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Borrowing principles of anhydrobiosis, we have developed a technique for self-assembling proteolipid-supported membranes on demand - simply by adding water. Intact lipid- and proteolipid vesicles dispersed in aqueous solutions of anhydrobiotic trehalose are vitrified on arbitrary substrates, producing glassy coats encapsulating biomolecules. Previous efforts establish that these carbohydrate coats arrest molecular mobilities and preserve native conformations and aggregative states of the embedded biomolecules, thereby enabling long-term storage. Subsequent rehydration, even after an extended period of time (e.g., weeks), devitrifies sugar - releasing the cargo and unmasking the substrate surface - thus triggering substrate-mediated vesicle fusion in real time, producing supported membranes. Using this method, arrays of membranes, including those functionalized with membrane proteins, can be readily produced in situ by spatially addressing vitrification using common patterning tools - useful for multiplexed or stochastic sensing and assaying of target interactions with the fluid and functional membrane surface.

Original languageEnglish (US)
Pages (from-to)60-63
Number of pages4
JournalJournal of the American Chemical Society
Volume136
Issue number1
DOIs
StatePublished - Jan 8 2014

Fingerprint

Sugars
Self assembly
Proteolipids
Membranes
Biomolecules
Substrates
Vitrification
Trehalose
Fluid Therapy
Conformations
Carbohydrates
Membrane Proteins
Fusion reactions
Lipids
Fluids
Water
Proteins

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

On-demand self-assembly of supported membranes using sacrificial, anhydrobiotic sugar coats. / Wilkop, Thomas E.; Sanborn, Jeremy; Oliver, Ann E.; Hanson, Joshua M.; Parikh, Atul N.

In: Journal of the American Chemical Society, Vol. 136, No. 1, 08.01.2014, p. 60-63.

Research output: Contribution to journalArticle

Wilkop, Thomas E. ; Sanborn, Jeremy ; Oliver, Ann E. ; Hanson, Joshua M. ; Parikh, Atul N. / On-demand self-assembly of supported membranes using sacrificial, anhydrobiotic sugar coats. In: Journal of the American Chemical Society. 2014 ; Vol. 136, No. 1. pp. 60-63.
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