On-bead combinatorial techniques for the identification of selective aldose reductase inhibitors

Lori I. Robins, Seth M. Dixon, David K. Wilson, Mark J. Kurth

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Aldose reductase (AKR1B1; ALR2; E.C. 1.1.1.21) is an NADPH-dependent carbonyl reductase which has long been associated with complications resulting from the elevated blood glucose often found in diabetics. The development of effective inhibitors has been plagued by lack of specificity which has led to side effects in clinical trials. To address this problem, a library of bead-immobilized compounds was screened against fluorescently labeled aldose reductase in the presence of fluorescently labeled aldehyde reductase, a non-target enzyme, to identify compounds which were aldose reductase specific. Picked beads were decoded via novel bifunctional bead mass spec-based techniques and kinetic analysis of the ten inhibitors which were identified using this protocol yielded IC50 values in the micromolar range. Most importantly, all of these compounds showed a preference for aldose reductase with selectivities as high as ∼7500-fold. The most potent of these exhibited uncompetitive inhibition versus the carbonyl-containing substrate d/l-glyceraldehyde with a Ki of 1.16 μM.

Original languageEnglish (US)
Pages (from-to)7728-7735
Number of pages8
JournalBioorganic and Medicinal Chemistry
Volume14
Issue number23
DOIs
StatePublished - Dec 1 2006

Keywords

  • Aldose reductase
  • Diabetes
  • Inhibitors
  • Selective on-bead assay

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science

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