Oligonucleotide-poly-L-ornithine conjugates: Binding to complementary DNA and RNA

T. Zhu, Z. Wei, C. H. Tung, W. A. Dickerhof, K. J. Breslauer, D. E. Georgopoulos, Michael J Leibowitz, S. Stein

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

On the basis of the reported enhanced antisense activity of polylysine-oligonucleotide conjugates, a synthetic 12-mer oligodeoxyribonucleotide has been coupled at its 5' terminus to a series of positively charged (δ-ornithine)(n) cysteine peptides. Binding between the nucleic acid-peptide conjugate and its complementary DNA target sequence was detected by the impact of complexation on the melting temperature (T(m)). It was found that the T(m) for the nucleic acid-peptide gradually increased with increasing net charge on the conjugated peptide. Site-directed cleavage with RNase H demonstrates that the peptide-modified oligomer also hybridizes with its RNA target sequence. Increased affinity for target mRNA with net charge was shown by a cell-free translation arrest assay.

Original languageEnglish (US)
Pages (from-to)265-275
Number of pages11
JournalAntisense Research and Development
Volume3
Issue number3
StatePublished - 1993
Externally publishedYes

Fingerprint

Complementary RNA
Oligonucleotides
Peptide Nucleic Acids
Complementary DNA
Peptides
Ribonuclease H
Temperature
Polylysine
Ornithine
Oligodeoxyribonucleotides
Complexation
Oligomers
Freezing
Cysteine
Melting point
Assays
RNA
Messenger RNA
polyornithine

ASJC Scopus subject areas

  • Genetics

Cite this

Zhu, T., Wei, Z., Tung, C. H., Dickerhof, W. A., Breslauer, K. J., Georgopoulos, D. E., ... Stein, S. (1993). Oligonucleotide-poly-L-ornithine conjugates: Binding to complementary DNA and RNA. Antisense Research and Development, 3(3), 265-275.

Oligonucleotide-poly-L-ornithine conjugates : Binding to complementary DNA and RNA. / Zhu, T.; Wei, Z.; Tung, C. H.; Dickerhof, W. A.; Breslauer, K. J.; Georgopoulos, D. E.; Leibowitz, Michael J; Stein, S.

In: Antisense Research and Development, Vol. 3, No. 3, 1993, p. 265-275.

Research output: Contribution to journalArticle

Zhu, T, Wei, Z, Tung, CH, Dickerhof, WA, Breslauer, KJ, Georgopoulos, DE, Leibowitz, MJ & Stein, S 1993, 'Oligonucleotide-poly-L-ornithine conjugates: Binding to complementary DNA and RNA', Antisense Research and Development, vol. 3, no. 3, pp. 265-275.
Zhu T, Wei Z, Tung CH, Dickerhof WA, Breslauer KJ, Georgopoulos DE et al. Oligonucleotide-poly-L-ornithine conjugates: Binding to complementary DNA and RNA. Antisense Research and Development. 1993;3(3):265-275.
Zhu, T. ; Wei, Z. ; Tung, C. H. ; Dickerhof, W. A. ; Breslauer, K. J. ; Georgopoulos, D. E. ; Leibowitz, Michael J ; Stein, S. / Oligonucleotide-poly-L-ornithine conjugates : Binding to complementary DNA and RNA. In: Antisense Research and Development. 1993 ; Vol. 3, No. 3. pp. 265-275.
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