Oligonucleotide-poly-L-ornithine conjugates: Binding to complementary DNA and RNA

T. Zhu, Z. Wei, C. H. Tung, W. A. Dickerhof, K. J. Breslauer, D. E. Georgopoulos, Michael J Leibowitz, S. Stein

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

On the basis of the reported enhanced antisense activity of polylysine-oligonucleotide conjugates, a synthetic 12-mer oligodeoxyribonucleotide has been coupled at its 5' terminus to a series of positively charged (δ-ornithine)(n) cysteine peptides. Binding between the nucleic acid-peptide conjugate and its complementary DNA target sequence was detected by the impact of complexation on the melting temperature (T(m)). It was found that the T(m) for the nucleic acid-peptide gradually increased with increasing net charge on the conjugated peptide. Site-directed cleavage with RNase H demonstrates that the peptide-modified oligomer also hybridizes with its RNA target sequence. Increased affinity for target mRNA with net charge was shown by a cell-free translation arrest assay.

Original languageEnglish (US)
Pages (from-to)265-275
Number of pages11
JournalAntisense Research and Development
Volume3
Issue number3
StatePublished - 1993
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

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    Zhu, T., Wei, Z., Tung, C. H., Dickerhof, W. A., Breslauer, K. J., Georgopoulos, D. E., Leibowitz, M. J., & Stein, S. (1993). Oligonucleotide-poly-L-ornithine conjugates: Binding to complementary DNA and RNA. Antisense Research and Development, 3(3), 265-275.