Oestrogen metabolites in relation to isoprostanes as a measure of oxidative stress

MaryFran Sowers, Daniel McConnell, Mary L. Jannausch, John F. Randolph, Robert Brook, Ellen B Gold, Sybil Crawford, Bill Lasley

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objective: Oestradiol (E2) and its metabolites 2-hydroxyoestrone (2-OHE1) and 16α-hydroxyoestrone (16α-OHE1) are thought to curtail the greater oxidative stress found in the development and progression of disease conditions including atherosclerosis. We related oestrogen levels to F 2a-isoprostane levels, a biomarker of oxidative stress. Design and participants: Data were obtained from 1647 women, aged 47-57 years, participating in the fifth annual follow-up of the Study of Women's Health Across the Nation (SWAN), a study of the menopausal transition. Measurements: Serum E2 and urinary 2-OHE1 and 16α-OHE1 concentrations were determined by enzyme-linked immunosorbent assay (ELISA) and urinary F2a- isoprostanes were measured by enzyme immunoassay (EIA). Results: F 2a-isoprostane concentrations were elevated in women who smoked, a behaviour associated with increased oxidative stress, but not in stages of the natural menopause. Mean F2a-isoprostane concentrations among pre- and postmenopausal women who smoked were 1082 and 1064 pg/ml, respectively, values double those in pre- (343 pg/ml) and postmenopausal (379 pg/ml) nonsmoking women. 2-OHE1 and F2a-isoprostane concentrations were positively and highly correlated (partial correlations ρY|X = 0.44 and ρY|X = 0.43 in pre- and postmenopausal women, respectively). Similarly, 16α-OHE1 concentrations were positively and highly correlated with F2a-isoprostane concentrations (ρY|X = 0.52 and ρY|X = 0.59 in pre- and postmenopausal women, respectively). E2 was significantly correlated with F2a-isoprostanes only in postmenopausal women (ρY|X = 0.20). Associations were adjusted for age, body mass index (BMI), race/ethnicity, lipids, physical activity level and alcohol consumption. Conclusions: This study does not support the commonly held hypothesis that levels of endogenous E2 or its oestrone metabolites favourably modify oxidative stress by decreasing F2a-isoprostane levels.

Original languageEnglish (US)
Pages (from-to)806-813
Number of pages8
JournalClinical Endocrinology
Volume68
Issue number5
DOIs
StatePublished - 2008

Fingerprint

Isoprostanes
Estrogens
Oxidative Stress
Estrone
Women's Health
Menopause
Immunoenzyme Techniques
Alcohol Drinking
Disease Progression
Estradiol
Atherosclerosis
Body Mass Index
Biomarkers
Enzyme-Linked Immunosorbent Assay
Exercise
Lipids

ASJC Scopus subject areas

  • Endocrinology

Cite this

Sowers, M., McConnell, D., Jannausch, M. L., Randolph, J. F., Brook, R., Gold, E. B., ... Lasley, B. (2008). Oestrogen metabolites in relation to isoprostanes as a measure of oxidative stress. Clinical Endocrinology, 68(5), 806-813. https://doi.org/10.1111/j.1365-2265.2007.03108.x

Oestrogen metabolites in relation to isoprostanes as a measure of oxidative stress. / Sowers, MaryFran; McConnell, Daniel; Jannausch, Mary L.; Randolph, John F.; Brook, Robert; Gold, Ellen B; Crawford, Sybil; Lasley, Bill.

In: Clinical Endocrinology, Vol. 68, No. 5, 2008, p. 806-813.

Research output: Contribution to journalArticle

Sowers, M, McConnell, D, Jannausch, ML, Randolph, JF, Brook, R, Gold, EB, Crawford, S & Lasley, B 2008, 'Oestrogen metabolites in relation to isoprostanes as a measure of oxidative stress', Clinical Endocrinology, vol. 68, no. 5, pp. 806-813. https://doi.org/10.1111/j.1365-2265.2007.03108.x
Sowers, MaryFran ; McConnell, Daniel ; Jannausch, Mary L. ; Randolph, John F. ; Brook, Robert ; Gold, Ellen B ; Crawford, Sybil ; Lasley, Bill. / Oestrogen metabolites in relation to isoprostanes as a measure of oxidative stress. In: Clinical Endocrinology. 2008 ; Vol. 68, No. 5. pp. 806-813.
@article{f777216f50a84f30b1c8155619bb8774,
title = "Oestrogen metabolites in relation to isoprostanes as a measure of oxidative stress",
abstract = "Objective: Oestradiol (E2) and its metabolites 2-hydroxyoestrone (2-OHE1) and 16α-hydroxyoestrone (16α-OHE1) are thought to curtail the greater oxidative stress found in the development and progression of disease conditions including atherosclerosis. We related oestrogen levels to F 2a-isoprostane levels, a biomarker of oxidative stress. Design and participants: Data were obtained from 1647 women, aged 47-57 years, participating in the fifth annual follow-up of the Study of Women's Health Across the Nation (SWAN), a study of the menopausal transition. Measurements: Serum E2 and urinary 2-OHE1 and 16α-OHE1 concentrations were determined by enzyme-linked immunosorbent assay (ELISA) and urinary F2a- isoprostanes were measured by enzyme immunoassay (EIA). Results: F 2a-isoprostane concentrations were elevated in women who smoked, a behaviour associated with increased oxidative stress, but not in stages of the natural menopause. Mean F2a-isoprostane concentrations among pre- and postmenopausal women who smoked were 1082 and 1064 pg/ml, respectively, values double those in pre- (343 pg/ml) and postmenopausal (379 pg/ml) nonsmoking women. 2-OHE1 and F2a-isoprostane concentrations were positively and highly correlated (partial correlations ρY|X = 0.44 and ρY|X = 0.43 in pre- and postmenopausal women, respectively). Similarly, 16α-OHE1 concentrations were positively and highly correlated with F2a-isoprostane concentrations (ρY|X = 0.52 and ρY|X = 0.59 in pre- and postmenopausal women, respectively). E2 was significantly correlated with F2a-isoprostanes only in postmenopausal women (ρY|X = 0.20). Associations were adjusted for age, body mass index (BMI), race/ethnicity, lipids, physical activity level and alcohol consumption. Conclusions: This study does not support the commonly held hypothesis that levels of endogenous E2 or its oestrone metabolites favourably modify oxidative stress by decreasing F2a-isoprostane levels.",
author = "MaryFran Sowers and Daniel McConnell and Jannausch, {Mary L.} and Randolph, {John F.} and Robert Brook and Gold, {Ellen B} and Sybil Crawford and Bill Lasley",
year = "2008",
doi = "10.1111/j.1365-2265.2007.03108.x",
language = "English (US)",
volume = "68",
pages = "806--813",
journal = "Clinical Endocrinology",
issn = "0300-0664",
publisher = "Wiley-Blackwell",
number = "5",

}

TY - JOUR

T1 - Oestrogen metabolites in relation to isoprostanes as a measure of oxidative stress

AU - Sowers, MaryFran

AU - McConnell, Daniel

AU - Jannausch, Mary L.

AU - Randolph, John F.

AU - Brook, Robert

AU - Gold, Ellen B

AU - Crawford, Sybil

AU - Lasley, Bill

PY - 2008

Y1 - 2008

N2 - Objective: Oestradiol (E2) and its metabolites 2-hydroxyoestrone (2-OHE1) and 16α-hydroxyoestrone (16α-OHE1) are thought to curtail the greater oxidative stress found in the development and progression of disease conditions including atherosclerosis. We related oestrogen levels to F 2a-isoprostane levels, a biomarker of oxidative stress. Design and participants: Data were obtained from 1647 women, aged 47-57 years, participating in the fifth annual follow-up of the Study of Women's Health Across the Nation (SWAN), a study of the menopausal transition. Measurements: Serum E2 and urinary 2-OHE1 and 16α-OHE1 concentrations were determined by enzyme-linked immunosorbent assay (ELISA) and urinary F2a- isoprostanes were measured by enzyme immunoassay (EIA). Results: F 2a-isoprostane concentrations were elevated in women who smoked, a behaviour associated with increased oxidative stress, but not in stages of the natural menopause. Mean F2a-isoprostane concentrations among pre- and postmenopausal women who smoked were 1082 and 1064 pg/ml, respectively, values double those in pre- (343 pg/ml) and postmenopausal (379 pg/ml) nonsmoking women. 2-OHE1 and F2a-isoprostane concentrations were positively and highly correlated (partial correlations ρY|X = 0.44 and ρY|X = 0.43 in pre- and postmenopausal women, respectively). Similarly, 16α-OHE1 concentrations were positively and highly correlated with F2a-isoprostane concentrations (ρY|X = 0.52 and ρY|X = 0.59 in pre- and postmenopausal women, respectively). E2 was significantly correlated with F2a-isoprostanes only in postmenopausal women (ρY|X = 0.20). Associations were adjusted for age, body mass index (BMI), race/ethnicity, lipids, physical activity level and alcohol consumption. Conclusions: This study does not support the commonly held hypothesis that levels of endogenous E2 or its oestrone metabolites favourably modify oxidative stress by decreasing F2a-isoprostane levels.

AB - Objective: Oestradiol (E2) and its metabolites 2-hydroxyoestrone (2-OHE1) and 16α-hydroxyoestrone (16α-OHE1) are thought to curtail the greater oxidative stress found in the development and progression of disease conditions including atherosclerosis. We related oestrogen levels to F 2a-isoprostane levels, a biomarker of oxidative stress. Design and participants: Data were obtained from 1647 women, aged 47-57 years, participating in the fifth annual follow-up of the Study of Women's Health Across the Nation (SWAN), a study of the menopausal transition. Measurements: Serum E2 and urinary 2-OHE1 and 16α-OHE1 concentrations were determined by enzyme-linked immunosorbent assay (ELISA) and urinary F2a- isoprostanes were measured by enzyme immunoassay (EIA). Results: F 2a-isoprostane concentrations were elevated in women who smoked, a behaviour associated with increased oxidative stress, but not in stages of the natural menopause. Mean F2a-isoprostane concentrations among pre- and postmenopausal women who smoked were 1082 and 1064 pg/ml, respectively, values double those in pre- (343 pg/ml) and postmenopausal (379 pg/ml) nonsmoking women. 2-OHE1 and F2a-isoprostane concentrations were positively and highly correlated (partial correlations ρY|X = 0.44 and ρY|X = 0.43 in pre- and postmenopausal women, respectively). Similarly, 16α-OHE1 concentrations were positively and highly correlated with F2a-isoprostane concentrations (ρY|X = 0.52 and ρY|X = 0.59 in pre- and postmenopausal women, respectively). E2 was significantly correlated with F2a-isoprostanes only in postmenopausal women (ρY|X = 0.20). Associations were adjusted for age, body mass index (BMI), race/ethnicity, lipids, physical activity level and alcohol consumption. Conclusions: This study does not support the commonly held hypothesis that levels of endogenous E2 or its oestrone metabolites favourably modify oxidative stress by decreasing F2a-isoprostane levels.

UR - http://www.scopus.com/inward/record.url?scp=42149117979&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42149117979&partnerID=8YFLogxK

U2 - 10.1111/j.1365-2265.2007.03108.x

DO - 10.1111/j.1365-2265.2007.03108.x

M3 - Article

C2 - 17980014

AN - SCOPUS:42149117979

VL - 68

SP - 806

EP - 813

JO - Clinical Endocrinology

JF - Clinical Endocrinology

SN - 0300-0664

IS - 5

ER -