Ocular coloboma and dorsoventral neuroretinal patterning defects in Lrp6 mutant eyes

Chengji Zhou, Andrei Molotkov, Lanying Song, Yunhong Li, David E Pleasure, Samuel J. Pleasure, Ya Zhou Wang

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Coloboma, an ocular birth defect seen in humans and other species, is caused by incomplete closure of the optic fissure. Here, we demonstrate that genetic deletion of Lrp6, a bottleneck coreceptor in the canonical Wnt signaling pathway, results in ocular coloboma and neuroretinal patterning defects in mice. The expression of ventral neuroretinal patterning gene Vax2 was conserved but with dorsally shifted expression domains; however, the dorsal neuroretinal patterning gene Tbx5 was lost in the Lrp6-mutant eyes at embryonic day 10.5. Both Bmp4 and phosphorylated Smad 1/5/8 were also significantly attenuated in the dorsal neuroretina. In addition, the retinoic acid synthesizing enzymes Raldh1 and Raldh3 were significantly changed in the mutant eyes. Our findings suggest that defective retinal patterning causes coloboma in the Lrp6-deficient mice, and that canonical Wnt signaling plays a primary role in dorsal neuroretinal patterning and related morphogenetic movements by regulation of both Bmp and retinoic acid signaling pathways.

Original languageEnglish (US)
Pages (from-to)3681-3689
Number of pages9
JournalDevelopmental Dynamics
Volume237
Issue number12
DOIs
StatePublished - Dec 2008

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Coloboma
Wnt Signaling Pathway
Tretinoin
Genes
Enzymes

Keywords

  • Bmp
  • Coloboma
  • Dorsoventral neuroretinal patterning
  • Lrp6
  • Raldh1
  • Raldh3
  • Retinoic acid
  • Tbx5
  • Vax2
  • Wnt

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Ocular coloboma and dorsoventral neuroretinal patterning defects in Lrp6 mutant eyes. / Zhou, Chengji; Molotkov, Andrei; Song, Lanying; Li, Yunhong; Pleasure, David E; Pleasure, Samuel J.; Wang, Ya Zhou.

In: Developmental Dynamics, Vol. 237, No. 12, 12.2008, p. 3681-3689.

Research output: Contribution to journalArticle

Zhou, Chengji ; Molotkov, Andrei ; Song, Lanying ; Li, Yunhong ; Pleasure, David E ; Pleasure, Samuel J. ; Wang, Ya Zhou. / Ocular coloboma and dorsoventral neuroretinal patterning defects in Lrp6 mutant eyes. In: Developmental Dynamics. 2008 ; Vol. 237, No. 12. pp. 3681-3689.
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