OCT methods for capillary velocimetry

Vivek Srinivasan, Harsha Radhakrishnan, Eng H. Lo, Emiri T. Mandeville, Alex E. Cable, Scott Barry

Research output: Contribution to journalArticle

82 Scopus citations

Abstract

To date, two main categories of OCT techniques have been described for imaging hemodynamics: Doppler OCT and OCT angiography. Doppler OCT can measure axial velocity profiles and flow in arteries and veins, while OCT angiography can determine vascular morphology, tone, and presence or absence of red blood cell (RBC) perfusion. However, neither method can quantify RBC velocity in capillaries, where RBC flow is typically transverse to the probe beam and single-file. Here, we describe new methods that potentially address these limitations. Firstly, we describe a complex-valued OCT signal in terms of a static scattering component, dynamic scattering component, and noise. Secondly, we propose that the time scale of random fluctuations in the dynamic scattering component are related to red blood cell velocity. Analysis was performed along the slow axis of repeated B-scans to parallelize measurements. We correlate our purported velocity measurements against two-photon microscopy measurements of RBC velocity, and investigate changes during hypercapnia. Finally, we image the ischemic stroke penumbra during distal middle cerebral artery occlusion (dMCAO), where OCT velocimetry methods provide additional insight that is not afforded by either Doppler OCT or OCT angiography.

Original languageEnglish (US)
Pages (from-to)612-629
Number of pages18
JournalBiomedical Optics Express
Volume3
Issue number3
DOIs
StatePublished - Mar 1 2012
Externally publishedYes

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ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Srinivasan, V., Radhakrishnan, H., Lo, E. H., Mandeville, E. T., Cable, A. E., & Barry, S. (2012). OCT methods for capillary velocimetry. Biomedical Optics Express, 3(3), 612-629. https://doi.org/10.1364/BOE.3.000612