Observing the deoxy myoglobin and hemogobin signals from rat myocardium in situ

Tuan Khanh Tran, Ulrike Kreutzer, Thomas Jue

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

1H NMR proximal histidyl N(δ)H signals of deoxy hemoglobin (Hb) and myoglobin (Mb) are distinguishable in the rat myocardium in situ. In the normoxic resting state, the blood and tissue pO2 is sufficient to saturate both Mb and Hb. No deoxy Mb or Hb signals are detected. Under 12% inspired O2, the erythrocyte Hb is partially desaturated and yields the α and β proximal histidyl N(δ)H signals of deoxy Hb. The detection of the Hb signals clarifies the debate about the NMR visibility of erythrocyte Hb in vivo and augments the strategy to observe tissue pO2. Copyright (C) 1998 Federation of European Biochemical Societies.

Original languageEnglish (US)
Pages (from-to)309-312
Number of pages4
JournalFEBS Letters
Volume434
Issue number3
DOIs
StatePublished - Sep 4 1998

Keywords

  • Hemoglobin
  • Myocardium
  • Myoglobin
  • Nuclear magnetic resonance

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Fingerprint Dive into the research topics of 'Observing the deoxy myoglobin and hemogobin signals from rat myocardium in situ'. Together they form a unique fingerprint.

  • Cite this