Observation of stripe superstructure in the β-two-phase coexistence region of cholesterol-phospholipid mixtures in supported membranes

Seyed R. Tabaei, Joshua A. Jackman, Bo Liedberg, Atul N. Parikh, Nam Joon Cho

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Visualization of phase coexistence in the βregion of cholesterol-phospholipid mixtures consisting of high cholesterol concentrations has proved elusive in lipid bilayers. Here, using the solvent-assisted lipid bilayer approach to prepare supported membranes with high cholesterol fractions close to the cholesterol solubility limit, we report the observation of coexisting liquid phases using fluorescence microscopy. At ∼63 mol % cholesterol, supported membranes consisting of mixtures of DOPC and cholesterol exhibit large-area striping reminiscent of the stripe superstructures that characterize the proximity of the second critical point in the miscibility phase diagram. The properties of the two phases are consistent with condensed complex-rich and cholesterol-rich liquids. Both phases exhibit long-range lateral mobility, and diffusion through a given phase is favored over hopping across the phase boundary, producing an "archipelago effect" and a complex percolation path.

Original languageEnglish (US)
Pages (from-to)16962-16965
Number of pages4
JournalJournal of the American Chemical Society
Volume136
Issue number49
DOIs
StatePublished - Dec 10 2014

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Cholesterol
Phospholipids
Observation
Membranes
Lipid bilayers
Lipid Bilayers
Solubility
Fluorescence microscopy
Liquids
Phase boundaries
Fluorescence Microscopy
Phase diagrams
Visualization

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Observation of stripe superstructure in the β-two-phase coexistence region of cholesterol-phospholipid mixtures in supported membranes. / Tabaei, Seyed R.; Jackman, Joshua A.; Liedberg, Bo; Parikh, Atul N.; Cho, Nam Joon.

In: Journal of the American Chemical Society, Vol. 136, No. 49, 10.12.2014, p. 16962-16965.

Research output: Contribution to journalArticle

Tabaei, Seyed R. ; Jackman, Joshua A. ; Liedberg, Bo ; Parikh, Atul N. ; Cho, Nam Joon. / Observation of stripe superstructure in the β-two-phase coexistence region of cholesterol-phospholipid mixtures in supported membranes. In: Journal of the American Chemical Society. 2014 ; Vol. 136, No. 49. pp. 16962-16965.
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