Oblique-Incidence Reflectivity Difference and Fluorescence Imaging of Oligonucleotide and IgG Protein Microarrays

James P. Landry, X. D. Zhu, X. W. Guo, Jeffrey Gregg

Research output: Chapter in Book/Report/Conference proceedingConference contribution

2 Scopus citations


We have constructed an oblique-incidence reflectivity difference (OI-RD) microscope for fluorescent label-free imaging of DNA and protein microarrays on standard glass substrates. Using both OI-RD and fluorescence images, we demonstrate a difference in wetting behavior of labeled and unlabeled IgG protein molecules deposited on an aldehyde-derivatized glass surface. The potential of fluorescent labeling agents to influence the properties of proteins highlights the need for label-free microarray detection techniques to supplement existing fluorescence methods. We also present OI-RD images of an oligonucleotide microarray after printing and washing procedures to demonstrate the use of OI-RD for non-destructive monitoring of changes in the optical properties of microarrays during processing.

Original languageEnglish (US)
Title of host publicationMaterials Research Society Symposium - Proceedings
EditorsC.S. Ozkan, J.T. Santini, H. Gao, G. Bao
Number of pages6
StatePublished - 2003
EventBiomicroelectromechanical Systems (BioMEMS) - San Francisco, CA, United States
Duration: Apr 22 2003Apr 25 2003


OtherBiomicroelectromechanical Systems (BioMEMS)
Country/TerritoryUnited States
CitySan Francisco, CA

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials


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