NudE and NudEL are required for mitotic progression and are involved in dynein recruitment to kinetochores

Stephanie A. Stehman, Yu Chen, Richard Mckenney, Richard B. Vallee

Research output: Contribution to journalArticlepeer-review

110 Scopus citations

Abstract

NudE and NudEL are related proteins that interact with cytoplasmic dynein and LIS1. Their functional relationship and involvement in LIS1 and dynein regulation are not completely understood. We find that NudE and NudEL each localize to mitotic kinetochores before dynein, dynactin, ZW10, and LIS1 and exhibit additional temporal and spatial differences in distribution from the motor protein. Inhibition of NudE and NudEL caused metaphase arrest with misoriented chromosomes and defective microtubule attachment. Dynein and dynactin were both displaced from kinetochores by the injection of an anti-NudE/NudEL antibody. Dynein but not dynactin interacted with NudE surprisingly through the dynein intermediate and light chains but not the motor domain. Together, these results identify a common function for NudE and NudEL in mitotic progression and identify an alternative mechanism for dynein recruitment to and regulation at kinetochores.

Original languageEnglish (US)
Pages (from-to)583-594
Number of pages12
JournalJournal of Cell Biology
Volume178
Issue number4
DOIs
StatePublished - Aug 13 2007
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology

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