Nucleotide sequence of the promoter and ammo-terminal coding region of the glutamate dehydrogenase structural gene of Escherichia coli

Fernando Valle, Elvira Sanvicente, Peter Seeburg, Alejandra Covarrubias, Raymond L. Rodriguez, Francisco Bolivar

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

A 610-bp DNA fragment carrying the promoter and amino-terminal coding regions of the glutamate dehydrogenase (GDH) structural gene from Escherichia coli has been sequenced. The amino-terminal sequence of the enzyme was also determined to help localize the transcriptional and translational signals for this gene. Three possible promoters and a CRP binding site were identified by concensus criteria. The sequence of 102 amino acids at the amino terminus of the enzyme is compared with the amino acid sequence from other GDH enzymes.

Original languageEnglish (US)
Pages (from-to)199-209
Number of pages11
JournalGene
Volume23
Issue number2
DOIs
StatePublished - 1983

Keywords

  • ammonia fixation
  • bacterial promoters
  • CRP binding site
  • DNA sequencing
  • protein homology
  • protein sequencing

ASJC Scopus subject areas

  • Genetics

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