Abstract
Using reverse transcription-polymerase chain reaction (RT-PCR), we have amplified vimentin (Vim)-encoding sequences from both total and polyadenylated bovine lens RNA. The amplified products were cloned and the nucleotide sequences determined. The amino-acid sequence of bovine vimentin shows 97.2, 96.8, 96.3 and 84.9% homology with reported aa sequences of human, mouse, hamster and chicken Vim, respectively.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 257-259 |
| Number of pages | 3 |
| Journal | Gene |
| Volume | 140 |
| Issue number | 2 |
| DOIs | |
| State | Published - Mar 25 1994 |
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Keywords
- Amino acid sequence homology
- bovine lens
- cytoskeleton
- epidermis
- intermediate filaments
ASJC Scopus subject areas
- Genetics
Cite this
Nucleotide sequence of the bovine vimentin-encoding cDNA. / Hess, John F.; Casselman, Jodi T.; FitzGerald, Paul G.
In: Gene, Vol. 140, No. 2, 25.03.1994, p. 257-259.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nucleotide sequence of the bovine vimentin-encoding cDNA
AU - Hess, John F.
AU - Casselman, Jodi T.
AU - FitzGerald, Paul G
PY - 1994/3/25
Y1 - 1994/3/25
N2 - Using reverse transcription-polymerase chain reaction (RT-PCR), we have amplified vimentin (Vim)-encoding sequences from both total and polyadenylated bovine lens RNA. The amplified products were cloned and the nucleotide sequences determined. The amino-acid sequence of bovine vimentin shows 97.2, 96.8, 96.3 and 84.9% homology with reported aa sequences of human, mouse, hamster and chicken Vim, respectively.
AB - Using reverse transcription-polymerase chain reaction (RT-PCR), we have amplified vimentin (Vim)-encoding sequences from both total and polyadenylated bovine lens RNA. The amplified products were cloned and the nucleotide sequences determined. The amino-acid sequence of bovine vimentin shows 97.2, 96.8, 96.3 and 84.9% homology with reported aa sequences of human, mouse, hamster and chicken Vim, respectively.
KW - Amino acid sequence homology
KW - bovine lens
KW - cytoskeleton
KW - epidermis
KW - intermediate filaments
UR - http://www.scopus.com/inward/record.url?scp=0028204279&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028204279&partnerID=8YFLogxK
U2 - 10.1016/0378-1119(94)90554-1
DO - 10.1016/0378-1119(94)90554-1
M3 - Article
VL - 140
SP - 257
EP - 259
JO - Gene
JF - Gene
SN - 0378-1119
IS - 2
ER -