Nucleotide sequence of the 3' terminal region of the LEU2 gene from Saccharomyces cerevisiae

Byron E. Froman, Robert C. Tait, Raymond L. Rodriguez

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The 3' terminal region from the LEU2 gene of yeast has been sequenced. Two open reading frames (ORF) have been identified, one of which constitutes the 3 ' terminus of β-isopropylmalate dehydrogenase, the product of the LEU2 gene. A noncoding spacer region located between the ORFs contains two consensus-type transcriptional terminators. The terminator-like sequences are oriented in opposing directions on opposite DNA strands. The noncoding spacer region may represent a single terminator for the LEU2 gene or two separate terminators involved in blocking convergent transcription from an as yet unidentified yeast gene.

Original languageEnglish (US)
Pages (from-to)257-261
Number of pages5
JournalGene
Volume31
Issue number1-3
DOIs
StatePublished - 1984

Keywords

  • consensus yeast terminator
  • dideoxysequencing
  • M13 cloning
  • open reading frames

ASJC Scopus subject areas

  • Genetics

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