Nucleotide sequence of cDNA containing the complete coding sequence for human lysosomal glucocerebrosidase

S. Tsuji, Prabhakara V Choudary, B. M. Martin, S. Winfield, J. A. Barranger, E. I. Ginns

Research output: Contribution to journalArticle

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Abstract

Complementary DNA clones for human glucocerebrosidase were isolated from a human hepatoma library in λgt11. The complete nucleotide sequence of the 1805-base pair cDNA insert has been determined. In addition to 5' and 3' untranslated regions (51 and 206 base pairs, respectively), the cDNA insert contains 1548 base pairs that completely encode human glucocerebrosidase. All possible N-linked glycosylation sites are identified. Examination of the 19 amino acids of the leader polypeptide beginning with the ATG at position 52 revealed a hydrophobic core and a carboxylterminal glycerine at the peptidase cleavage site, features consistent with the leader sequences described for other human translocated proteins. The M(r) of 57,000 calculated from the 516 amino acids deduced from cDNA sequence is in good agreement with that identified by immunoprecipitation following in vitro translation of human placental mRNA.

Original languageEnglish (US)
Pages (from-to)50-53
Number of pages4
JournalJournal of Biological Chemistry
Volume261
Issue number1
StatePublished - 1986
Externally publishedYes

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Glucosylceramidase
Nucleotides
Complementary DNA
Base Pairing
Glycosylation
Amino Acids
5' Untranslated Regions
3' Untranslated Regions
Glycerol
Peptide Hydrolases
Immunoprecipitation
Libraries
Hepatocellular Carcinoma
Messenger RNA
Clone Cells
Peptides
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Tsuji, S., Choudary, P. V., Martin, B. M., Winfield, S., Barranger, J. A., & Ginns, E. I. (1986). Nucleotide sequence of cDNA containing the complete coding sequence for human lysosomal glucocerebrosidase. Journal of Biological Chemistry, 261(1), 50-53.

Nucleotide sequence of cDNA containing the complete coding sequence for human lysosomal glucocerebrosidase. / Tsuji, S.; Choudary, Prabhakara V; Martin, B. M.; Winfield, S.; Barranger, J. A.; Ginns, E. I.

In: Journal of Biological Chemistry, Vol. 261, No. 1, 1986, p. 50-53.

Research output: Contribution to journalArticle

Tsuji, S, Choudary, PV, Martin, BM, Winfield, S, Barranger, JA & Ginns, EI 1986, 'Nucleotide sequence of cDNA containing the complete coding sequence for human lysosomal glucocerebrosidase', Journal of Biological Chemistry, vol. 261, no. 1, pp. 50-53.
Tsuji, S. ; Choudary, Prabhakara V ; Martin, B. M. ; Winfield, S. ; Barranger, J. A. ; Ginns, E. I. / Nucleotide sequence of cDNA containing the complete coding sequence for human lysosomal glucocerebrosidase. In: Journal of Biological Chemistry. 1986 ; Vol. 261, No. 1. pp. 50-53.
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