Abstract
Complementary DNA clones for human glucocerebrosidase were isolated from a human hepatoma library in λgt11. The complete nucleotide sequence of the 1805-base pair cDNA insert has been determined. In addition to 5' and 3' untranslated regions (51 and 206 base pairs, respectively), the cDNA insert contains 1548 base pairs that completely encode human glucocerebrosidase. All possible N-linked glycosylation sites are identified. Examination of the 19 amino acids of the leader polypeptide beginning with the ATG at position 52 revealed a hydrophobic core and a carboxylterminal glycerine at the peptidase cleavage site, features consistent with the leader sequences described for other human translocated proteins. The M(r) of 57,000 calculated from the 516 amino acids deduced from cDNA sequence is in good agreement with that identified by immunoprecipitation following in vitro translation of human placental mRNA.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 50-53 |
| Number of pages | 4 |
| Journal | Journal of Biological Chemistry |
| Volume | 261 |
| Issue number | 1 |
| State | Published - 1986 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- Biochemistry
Cite this
Nucleotide sequence of cDNA containing the complete coding sequence for human lysosomal glucocerebrosidase. / Tsuji, S.; Choudary, Prabhakara V; Martin, B. M.; Winfield, S.; Barranger, J. A.; Ginns, E. I.
In: Journal of Biological Chemistry, Vol. 261, No. 1, 1986, p. 50-53.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nucleotide sequence of cDNA containing the complete coding sequence for human lysosomal glucocerebrosidase
AU - Tsuji, S.
AU - Choudary, Prabhakara V
AU - Martin, B. M.
AU - Winfield, S.
AU - Barranger, J. A.
AU - Ginns, E. I.
PY - 1986
Y1 - 1986
N2 - Complementary DNA clones for human glucocerebrosidase were isolated from a human hepatoma library in λgt11. The complete nucleotide sequence of the 1805-base pair cDNA insert has been determined. In addition to 5' and 3' untranslated regions (51 and 206 base pairs, respectively), the cDNA insert contains 1548 base pairs that completely encode human glucocerebrosidase. All possible N-linked glycosylation sites are identified. Examination of the 19 amino acids of the leader polypeptide beginning with the ATG at position 52 revealed a hydrophobic core and a carboxylterminal glycerine at the peptidase cleavage site, features consistent with the leader sequences described for other human translocated proteins. The M(r) of 57,000 calculated from the 516 amino acids deduced from cDNA sequence is in good agreement with that identified by immunoprecipitation following in vitro translation of human placental mRNA.
AB - Complementary DNA clones for human glucocerebrosidase were isolated from a human hepatoma library in λgt11. The complete nucleotide sequence of the 1805-base pair cDNA insert has been determined. In addition to 5' and 3' untranslated regions (51 and 206 base pairs, respectively), the cDNA insert contains 1548 base pairs that completely encode human glucocerebrosidase. All possible N-linked glycosylation sites are identified. Examination of the 19 amino acids of the leader polypeptide beginning with the ATG at position 52 revealed a hydrophobic core and a carboxylterminal glycerine at the peptidase cleavage site, features consistent with the leader sequences described for other human translocated proteins. The M(r) of 57,000 calculated from the 516 amino acids deduced from cDNA sequence is in good agreement with that identified by immunoprecipitation following in vitro translation of human placental mRNA.
UR - http://www.scopus.com/inward/record.url?scp=0022650774&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0022650774&partnerID=8YFLogxK
M3 - Article
C2 - 3001061
AN - SCOPUS:0022650774
VL - 261
SP - 50
EP - 53
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 1
ER -