Nucleotide sequence and expression of an AIDS-Associated Retrovirus (ARV-2)

Ray Sanchez-Pescador, Michael D. Power, Philip J. Barr, Kathelyn S. Steimer, Michelle M. Stempien, Sheryl L. Brown-Shimer, Wendy W. Gee, Andre Renard, Anne Randolph, Jay A. Levy, Dino Dina, Paul A Luciw

Research output: Contribution to journalArticle

453 Scopus citations

Abstract

The nucleotide sequence of molecular clones of DNA from a retrovirus, ARV-2, associated with the acquired immune deficiency syndrome (AIDS) was determined. Proviral DNA of ARV-2 (9737 base pairs) has long terminal repeat structures (636 base pairs) and long open reading frames encoding gag (505 codons), pol (1003 codons), and env (863 codons) genes. Two additional open reading frames were identified. Significant amino acid homology with several other retroviruses was noted in the predicted product of gag and pol, but ARV-2 was as closely related to murine and avian retroviruses as it was to human T-cell leukemia viruses (HTLV-I and HTLV-II). By means of an SV-40 vector in transfected simian cells, the cloned gag and env genes of ARV-2 were shown to express viral proteins.

Original languageEnglish (US)
Pages (from-to)484-492
Number of pages9
JournalScience
Volume227
Issue number4686
StatePublished - 1985

ASJC Scopus subject areas

  • Medicine(all)
  • General

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