Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12)

Daniela Fehr; Gregg A. Dean; Jon Huder; Zhanyun Fan; Silke Huettner; Joanne W. Higgins; Niels C Pedersen; Hans Lutz

doi:10.3109/10425179709020889

Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12)

Daniela Fehr, Gregg A. Dean, Jon Huder, Zhanyun Fan, Silke Huettner, Joanne W. Higgins, Niels C Pedersen, Hans Lutz

Medicine and Epidemiology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Feline Interleukin-12 (IL-12) is a heterodimeric glycoprotein consisting of two disulfide linked subunits of about 40kD (p40) and 35kD (p35). It is a pleiotropic cytokine mediating biological activities on T- and NK-cells. One important function is the induction of a Thl immune response. Here we report the cloning and sequencing of feline IL-12, the expression of the p40-protein in E. coli and production of monoclonal antibodies. At the nucleotide level, feline IL-12 shows between 87-90%, on the amino acid level between 82-87% identity to the bovine and human IL-12, respectively.

Original language	English (US)
Pages (from-to)	77-82
Number of pages	6
Journal	Mitochondrial DNA
Volume	8
Issue number	1-2
DOIs	https://doi.org/10.3109/10425179709020889
State	Published - 1997

Keywords

Cat
cytokine
feline
interleukin-12
sequence

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.3109/10425179709020889

Cite this

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title = "Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12)",

abstract = "Feline Interleukin-12 (IL-12) is a heterodimeric glycoprotein consisting of two disulfide linked subunits of about 40kD (p40) and 35kD (p35). It is a pleiotropic cytokine mediating biological activities on T- and NK-cells. One important function is the induction of a Thl immune response. Here we report the cloning and sequencing of feline IL-12, the expression of the p40-protein in E. coli and production of monoclonal antibodies. At the nucleotide level, feline IL-12 shows between 87-90%, on the amino acid level between 82-87% identity to the bovine and human IL-12, respectively.",

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T1 - Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12)

AU - Fehr, Daniela

AU - Dean, Gregg A.

AU - Huder, Jon

AU - Fan, Zhanyun

AU - Huettner, Silke

AU - Higgins, Joanne W.

AU - Pedersen, Niels C

AU - Lutz, Hans

PY - 1997

Y1 - 1997

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AB - Feline Interleukin-12 (IL-12) is a heterodimeric glycoprotein consisting of two disulfide linked subunits of about 40kD (p40) and 35kD (p35). It is a pleiotropic cytokine mediating biological activities on T- and NK-cells. One important function is the induction of a Thl immune response. Here we report the cloning and sequencing of feline IL-12, the expression of the p40-protein in E. coli and production of monoclonal antibodies. At the nucleotide level, feline IL-12 shows between 87-90%, on the amino acid level between 82-87% identity to the bovine and human IL-12, respectively.

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KW - interleukin-12

KW - sequence

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Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12)

Abstract

Keywords

ASJC Scopus subject areas

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