Abstract
Feline Interleukin-12 (IL-12) is a heterodimeric glycoprotein consisting of two disulfide linked subunits of about 40kD (p40) and 35kD (p35). It is a pleiotropic cytokine mediating biological activities on T- and NK-cells. One important function is the induction of a Thl immune response. Here we report the cloning and sequencing of feline IL-12, the expression of the p40-protein in E. coli and production of monoclonal antibodies. At the nucleotide level, feline IL-12 shows between 87-90%, on the amino acid level between 82-87% identity to the bovine and human IL-12, respectively.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 77-82 |
| Number of pages | 6 |
| Journal | Mitochondrial DNA |
| Volume | 8 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - 1997 |
Keywords
- Cat
- cytokine
- feline
- interleukin-12
- sequence
ASJC Scopus subject areas
- Molecular Biology
- Genetics
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Fehr, D., Dean, G. A., Huder, J., Fan, Z., Huettner, S., Higgins, J. W., Pedersen, N. C., & Lutz, H. (1997). Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12). Mitochondrial DNA, 8(1-2), 77-82. https://doi.org/10.3109/10425179709020889