Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12)

Daniela Fehr, Gregg A. Dean, Jon Huder, Zhanyun Fan, Silke Huettner, Joanne W. Higgins, Niels C Pedersen, Hans Lutz

Research output: Contribution to journal › Article

6 Citations (Scopus)

Abstract

Feline Interleukin-12 (IL-12) is a heterodimeric glycoprotein consisting of two disulfide linked subunits of about 40kD (p40) and 35kD (p35). It is a pleiotropic cytokine mediating biological activities on T- and NK-cells. One important function is the induction of a Thl immune response. Here we report the cloning and sequencing of feline IL-12, the expression of the p40-protein in E. coli and production of monoclonal antibodies. At the nucleotide level, feline IL-12 shows between 87-90%, on the amino acid level between 82-87% identity to the bovine and human IL-12, respectively.

Original languageEnglish (US)
Pages (from-to)77-82
Number of pages6
JournalMitochondrial DNA
Volume8
Issue number1-2
DOIs
StatePublished - 1997

Fingerprint

Felidae
Interleukin-12
Nucleotides
Peptides
Escherichia coli Proteins
Natural Killer Cells
Disulfides
Organism Cloning
Glycoproteins
Monoclonal Antibodies
Cytokines
Amino Acids
adjuvant P40

Keywords

  • Cat
  • cytokine
  • feline
  • interleukin-12
  • sequence

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Fehr, D., Dean, G. A., Huder, J., Fan, Z., Huettner, S., Higgins, J. W., ... Lutz, H. (1997). Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12). Mitochondrial DNA, 8(1-2), 77-82. https://doi.org/10.3109/10425179709020889

Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12). / Fehr, Daniela; Dean, Gregg A.; Huder, Jon; Fan, Zhanyun; Huettner, Silke; Higgins, Joanne W.; Pedersen, Niels C; Lutz, Hans.

In: Mitochondrial DNA, Vol. 8, No. 1-2, 1997, p. 77-82.

Research output: Contribution to journal › Article

Fehr, D, Dean, GA, Huder, J, Fan, Z, Huettner, S, Higgins, JW, Pedersen, NC & Lutz, H 1997, 'Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12)', Mitochondrial DNA, vol. 8, no. 1-2, pp. 77-82. https://doi.org/10.3109/10425179709020889
Fehr D, Dean GA, Huder J, Fan Z, Huettner S, Higgins JW et al. Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12). Mitochondrial DNA. 1997;8(1-2):77-82. https://doi.org/10.3109/10425179709020889
Fehr, Daniela ; Dean, Gregg A. ; Huder, Jon ; Fan, Zhanyun ; Huettner, Silke ; Higgins, Joanne W. ; Pedersen, Niels C ; Lutz, Hans. / Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12). In: Mitochondrial DNA. 1997 ; Vol. 8, No. 1-2. pp. 77-82.
@article{3037f5f72373483a81490234161443b1,
title = "Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12)",
abstract = "Feline Interleukin-12 (IL-12) is a heterodimeric glycoprotein consisting of two disulfide linked subunits of about 40kD (p40) and 35kD (p35). It is a pleiotropic cytokine mediating biological activities on T- and NK-cells. One important function is the induction of a Thl immune response. Here we report the cloning and sequencing of feline IL-12, the expression of the p40-protein in E. coli and production of monoclonal antibodies. At the nucleotide level, feline IL-12 shows between 87-90{\%}, on the amino acid level between 82-87{\%} identity to the bovine and human IL-12, respectively.",
keywords = "Cat, cytokine, feline, interleukin-12, sequence",
author = "Daniela Fehr and Dean, {Gregg A.} and Jon Huder and Zhanyun Fan and Silke Huettner and Higgins, {Joanne W.} and Pedersen, {Niels C} and Hans Lutz",
year = "1997",
doi = "10.3109/10425179709020889",
language = "English (US)",
volume = "8",
pages = "77--82",
journal = "DNA Sequence - Journal of DNA Sequencing and Mapping",
issn = "1940-1736",
publisher = "Informa Healthcare",
number = "1-2",

}

TY - JOUR

T1 - Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12)

AU - Fehr, Daniela

AU - Dean, Gregg A.

AU - Huder, Jon

AU - Fan, Zhanyun

AU - Huettner, Silke

AU - Higgins, Joanne W.

AU - Pedersen, Niels C

AU - Lutz, Hans

PY - 1997

Y1 - 1997

N2 - Feline Interleukin-12 (IL-12) is a heterodimeric glycoprotein consisting of two disulfide linked subunits of about 40kD (p40) and 35kD (p35). It is a pleiotropic cytokine mediating biological activities on T- and NK-cells. One important function is the induction of a Thl immune response. Here we report the cloning and sequencing of feline IL-12, the expression of the p40-protein in E. coli and production of monoclonal antibodies. At the nucleotide level, feline IL-12 shows between 87-90%, on the amino acid level between 82-87% identity to the bovine and human IL-12, respectively.

AB - Feline Interleukin-12 (IL-12) is a heterodimeric glycoprotein consisting of two disulfide linked subunits of about 40kD (p40) and 35kD (p35). It is a pleiotropic cytokine mediating biological activities on T- and NK-cells. One important function is the induction of a Thl immune response. Here we report the cloning and sequencing of feline IL-12, the expression of the p40-protein in E. coli and production of monoclonal antibodies. At the nucleotide level, feline IL-12 shows between 87-90%, on the amino acid level between 82-87% identity to the bovine and human IL-12, respectively.

KW - Cat

KW - cytokine

KW - feline

KW - interleukin-12

KW - sequence

UR - http://www.scopus.com/inward/record.url?scp=0031447790&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031447790&partnerID=8YFLogxK

U2 - 10.3109/10425179709020889

DO - 10.3109/10425179709020889

M3 - Article

C2 - 9522125

AN - SCOPUS:0031447790

VL - 8

SP - 77

EP - 82

JO - DNA Sequence - Journal of DNA Sequencing and Mapping

JF - DNA Sequence - Journal of DNA Sequencing and Mapping

SN - 1940-1736

IS - 1-2

ER -

Access to Document