Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12)

Daniela Fehr, Gregg A. Dean, Jon Huder, Zhanyun Fan, Silke Huettner, Joanne W. Higgins, Niels C Pedersen, Hans Lutz

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Feline Interleukin-12 (IL-12) is a heterodimeric glycoprotein consisting of two disulfide linked subunits of about 40kD (p40) and 35kD (p35). It is a pleiotropic cytokine mediating biological activities on T- and NK-cells. One important function is the induction of a Thl immune response. Here we report the cloning and sequencing of feline IL-12, the expression of the p40-protein in E. coli and production of monoclonal antibodies. At the nucleotide level, feline IL-12 shows between 87-90%, on the amino acid level between 82-87% identity to the bovine and human IL-12, respectively.

Original languageEnglish (US)
Pages (from-to)77-82
Number of pages6
JournalMitochondrial DNA
Volume8
Issue number1-2
DOIs
StatePublished - 1997

Keywords

  • Cat
  • cytokine
  • feline
  • interleukin-12
  • sequence

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Fehr, D., Dean, G. A., Huder, J., Fan, Z., Huettner, S., Higgins, J. W., Pedersen, N. C., & Lutz, H. (1997). Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12). Mitochondrial DNA, 8(1-2), 77-82. https://doi.org/10.3109/10425179709020889