Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12)

Daniela Fehr, Gregg A. Dean, Jon Huder, Zhanyun Fan, Silke Huettner, Joanne W. Higgins, Niels C Pedersen, Hans Lutz

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Feline Interleukin-12 (IL-12) is a heterodimeric glycoprotein consisting of two disulfide linked subunits of about 40kD (p40) and 35kD (p35). It is a pleiotropic cytokine mediating biological activities on T- and NK-cells. One important function is the induction of a Thl immune response. Here we report the cloning and sequencing of feline IL-12, the expression of the p40-protein in E. coli and production of monoclonal antibodies. At the nucleotide level, feline IL-12 shows between 87-90%, on the amino acid level between 82-87% identity to the bovine and human IL-12, respectively.

Original languageEnglish (US)
Pages (from-to)77-82
Number of pages6
JournalMitochondrial DNA
Volume8
Issue number1-2
DOIs
StatePublished - 1997

Fingerprint

Felidae
Interleukin-12
Nucleotides
Peptides
Escherichia coli Proteins
Natural Killer Cells
Disulfides
Organism Cloning
Glycoproteins
Monoclonal Antibodies
Cytokines
Amino Acids
adjuvant P40

Keywords

  • Cat
  • cytokine
  • feline
  • interleukin-12
  • sequence

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Fehr, D., Dean, G. A., Huder, J., Fan, Z., Huettner, S., Higgins, J. W., ... Lutz, H. (1997). Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12). Mitochondrial DNA, 8(1-2), 77-82. https://doi.org/10.3109/10425179709020889

Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12). / Fehr, Daniela; Dean, Gregg A.; Huder, Jon; Fan, Zhanyun; Huettner, Silke; Higgins, Joanne W.; Pedersen, Niels C; Lutz, Hans.

In: Mitochondrial DNA, Vol. 8, No. 1-2, 1997, p. 77-82.

Research output: Contribution to journalArticle

Fehr, D, Dean, GA, Huder, J, Fan, Z, Huettner, S, Higgins, JW, Pedersen, NC & Lutz, H 1997, 'Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12)', Mitochondrial DNA, vol. 8, no. 1-2, pp. 77-82. https://doi.org/10.3109/10425179709020889
Fehr, Daniela ; Dean, Gregg A. ; Huder, Jon ; Fan, Zhanyun ; Huettner, Silke ; Higgins, Joanne W. ; Pedersen, Niels C ; Lutz, Hans. / Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12). In: Mitochondrial DNA. 1997 ; Vol. 8, No. 1-2. pp. 77-82.
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