Nucleocapsid and glycoprotein organization in an enveloped virus

R. Holland Cheng, Richard J. Kuhn, Norman H. Olson, Michael G. Rossmann^Hok-Kin Choi, Thomas J. Smith, Timothy S. Baker

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253 Scopus citations

Abstract

Alphaviruses are a group of icosahedral, positive-strand RNA, enveloped viruses. The membrane bilayer, which surrounds the ∼ 400 Å diameter nucleocapsid, is penetrated by 80 spikes arranged in a T = 4 lattice. Each spike is a trimer of heterodimers consisting of glycoproteins Ell and E2. Cryoelectron microscopy and image reconstruction of Ross River virus showed that the T=4 quaternary structure of the nucleocapsid consists of pentamerand hexamer clusters of the capsid protein, but not dimers, as have been observed in several crystallographic studies. The E1-E2 heterodimers form one-to-one associations with the nucleocapsid monomers across the lipid bilayer. Knowledge of the atomic structure of the capsid protein and our reconstruction allows us to identify capsid-protein residues that interact with the RNA, the glycoproteins, and adjacent capsid-proteins.

Original languageEnglish (US)
Pages (from-to)621-630
Number of pages10
JournalCell
Volume80
Issue number4
DOIs
StatePublished - Feb 24 1995
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Cell Biology
  • Molecular Biology

Cite this

Holland Cheng, R., Kuhn, R. J., Olson, N. H., Rossmann^Hok-Kin Choi, M. G., Smith, T. J., & Baker, T. S. (1995). Nucleocapsid and glycoprotein organization in an enveloped virus. Cell, 80(4), 621-630. https://doi.org/10.1016/0092-8674(95)90516-2