TY - JOUR
T1 - Nucleocapsid and glycoprotein organization in an enveloped virus
AU - Holland Cheng, R.
AU - Kuhn, Richard J.
AU - Olson, Norman H.
AU - Rossmann^Hok-Kin Choi, Michael G.
AU - Smith, Thomas J.
AU - Baker, Timothy S.
PY - 1995/2/24
Y1 - 1995/2/24
N2 - Alphaviruses are a group of icosahedral, positive-strand RNA, enveloped viruses. The membrane bilayer, which surrounds the ∼ 400 Å diameter nucleocapsid, is penetrated by 80 spikes arranged in a T = 4 lattice. Each spike is a trimer of heterodimers consisting of glycoproteins Ell and E2. Cryoelectron microscopy and image reconstruction of Ross River virus showed that the T=4 quaternary structure of the nucleocapsid consists of pentamerand hexamer clusters of the capsid protein, but not dimers, as have been observed in several crystallographic studies. The E1-E2 heterodimers form one-to-one associations with the nucleocapsid monomers across the lipid bilayer. Knowledge of the atomic structure of the capsid protein and our reconstruction allows us to identify capsid-protein residues that interact with the RNA, the glycoproteins, and adjacent capsid-proteins.
AB - Alphaviruses are a group of icosahedral, positive-strand RNA, enveloped viruses. The membrane bilayer, which surrounds the ∼ 400 Å diameter nucleocapsid, is penetrated by 80 spikes arranged in a T = 4 lattice. Each spike is a trimer of heterodimers consisting of glycoproteins Ell and E2. Cryoelectron microscopy and image reconstruction of Ross River virus showed that the T=4 quaternary structure of the nucleocapsid consists of pentamerand hexamer clusters of the capsid protein, but not dimers, as have been observed in several crystallographic studies. The E1-E2 heterodimers form one-to-one associations with the nucleocapsid monomers across the lipid bilayer. Knowledge of the atomic structure of the capsid protein and our reconstruction allows us to identify capsid-protein residues that interact with the RNA, the glycoproteins, and adjacent capsid-proteins.
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U2 - 10.1016/0092-8674(95)90516-2
DO - 10.1016/0092-8674(95)90516-2
M3 - Article
C2 - 7867069
AN - SCOPUS:0028919758
VL - 80
SP - 621
EP - 630
JO - Cell
JF - Cell
SN - 0092-8674
IS - 4
ER -