Nucleic acid, antibody, and virus culture methods to detect xenotropic mlv-related virus in human blood samples

M. F. Kearney, K. Lee, R. K. Bagni, A. Wiegand, J. Spindler, F. Maldarelli, P. A. Pinto, W. M. Linehan, C. D. Vocke, K. A. Delviks-Frankenberry, Ralph W deVere White, G. Q. Del Prete, J. W. Mellors, J. D. Lifson, V. N. Kewalramani, V. K. Pathak, J. M. Coffin, S. F J Le Grice

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

The MLV-related retrovirus, XMRV, was recently identified and reported to be associated with both prostate cancer and chronic fatigue syndrome. At the National Cancer Institute-Frederick, MD (NCI-Frederick), we developed highly sensitive methods to detect XMRV nucleic acids, antibodies, and replication competent virus. Analysis of XMRV-spiked samples and/or specimens from two pigtail macaques experimentally inoculated with 22Rv1 cell-derived XMRV confirmed the ability of the assays used to detect XMRV RNA and DNA, and culture isolatable virus when present, along with XMRV reactive antibody responses. Using these assays, we did not detect evidence of XMRV in blood samples (N=134) or prostate specimens (N=19) from two independent cohorts of patients with prostate cancer. Previous studies detected XMRV in prostate tissues. In the present study, we primarily investigated the levels of XMRV in blood plasma samples collected from patients with prostate cancer. These results demonstrate that while XMRV-related assays developed at the NCI-Frederick can readily measure XMRV nucleic acids, antibodies, and replication competent virus, no evidence of XMRV was found in the blood of patients with prostate cancer.

Original languageEnglish (US)
Article number272193
JournalAdvances in Virology
Volume2011
DOIs
StatePublished - 2011

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

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    Kearney, M. F., Lee, K., Bagni, R. K., Wiegand, A., Spindler, J., Maldarelli, F., Pinto, P. A., Linehan, W. M., Vocke, C. D., Delviks-Frankenberry, K. A., deVere White, R. W., Del Prete, G. Q., Mellors, J. W., Lifson, J. D., Kewalramani, V. N., Pathak, V. K., Coffin, J. M., & Le Grice, S. F. J. (2011). Nucleic acid, antibody, and virus culture methods to detect xenotropic mlv-related virus in human blood samples. Advances in Virology, 2011, [272193]. https://doi.org/10.1155/2011/272193