Abstract
Objective: To compare cumulus cell structure and timing of oocyte maturation of in vitro-matured (IVM) and in vivo-matured (VVM) nonhuman primate oocytes. Design: In vivo maturation and in vitro maturation of oocytes. Setting: Animal cell culture laboratory. Animal(s): Forty-eight female rhesus macaques. Intervention(s): Fifteen animals were administered FSH, and aspirated oocytes were cultured in vitro for 0, 3, 6, 12, or 24 hours (IVM). Thirty-three animals were administered FSH and hCG, and oocytes were collected 3, 6, 12, or 28-30 hours after hCG (VVM). Main Outcome Measure(s): Nuclear maturation and microtubule scores of oocytes and actin and tubulin transzonal processes of cumulus cells. Embryo development was observed for VVM oocytes. Result(s): The rate of nuclear maturation was faster for IVM oocytes compared with VVM oocytes. Actin transzonal processes decreased 0-12 hours after hCG administration for VVM oocytes. Tubulin transzonal processes of IVM and VVM oocytes decreased from 0 to 24 hours and from 0 to 3 hours, respectively. Embryo development improved as VVM time increased. Conclusion(s): Nuclear maturation and remodeling of cumulus-oocyte complex structural components associated with in vitro maturation do not parallel those of oocyte maturation in vivo, indicating that in vitro culture conditions continue to be suboptimal.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2043-2050 |
| Number of pages | 8 |
| Journal | Fertility and Sterility |
| Volume | 91 |
| Issue number | 5 SUPPL. |
| DOIs | |
| State | Published - May 2009 |
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Keywords
- actin
- ovary
- Rhesus monkey
- spindle
- tubulin
- zona pellucida
ASJC Scopus subject areas
- Obstetrics and Gynecology
- Reproductive Medicine
Cite this
Nuclear maturation and structural components of nonhuman primate cumulus-oocyte complexes during in vivo and in vitro maturation. / Nyholt de Prada, Jenna K.; Hill, Dana L.; Chaffin, Charles L.; VandeVoort, Catherine A.
In: Fertility and Sterility, Vol. 91, No. 5 SUPPL., 05.2009, p. 2043-2050.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nuclear maturation and structural components of nonhuman primate cumulus-oocyte complexes during in vivo and in vitro maturation
AU - Nyholt de Prada, Jenna K.
AU - Hill, Dana L.
AU - Chaffin, Charles L.
AU - VandeVoort, Catherine A.
PY - 2009/5
Y1 - 2009/5
N2 - Objective: To compare cumulus cell structure and timing of oocyte maturation of in vitro-matured (IVM) and in vivo-matured (VVM) nonhuman primate oocytes. Design: In vivo maturation and in vitro maturation of oocytes. Setting: Animal cell culture laboratory. Animal(s): Forty-eight female rhesus macaques. Intervention(s): Fifteen animals were administered FSH, and aspirated oocytes were cultured in vitro for 0, 3, 6, 12, or 24 hours (IVM). Thirty-three animals were administered FSH and hCG, and oocytes were collected 3, 6, 12, or 28-30 hours after hCG (VVM). Main Outcome Measure(s): Nuclear maturation and microtubule scores of oocytes and actin and tubulin transzonal processes of cumulus cells. Embryo development was observed for VVM oocytes. Result(s): The rate of nuclear maturation was faster for IVM oocytes compared with VVM oocytes. Actin transzonal processes decreased 0-12 hours after hCG administration for VVM oocytes. Tubulin transzonal processes of IVM and VVM oocytes decreased from 0 to 24 hours and from 0 to 3 hours, respectively. Embryo development improved as VVM time increased. Conclusion(s): Nuclear maturation and remodeling of cumulus-oocyte complex structural components associated with in vitro maturation do not parallel those of oocyte maturation in vivo, indicating that in vitro culture conditions continue to be suboptimal.
AB - Objective: To compare cumulus cell structure and timing of oocyte maturation of in vitro-matured (IVM) and in vivo-matured (VVM) nonhuman primate oocytes. Design: In vivo maturation and in vitro maturation of oocytes. Setting: Animal cell culture laboratory. Animal(s): Forty-eight female rhesus macaques. Intervention(s): Fifteen animals were administered FSH, and aspirated oocytes were cultured in vitro for 0, 3, 6, 12, or 24 hours (IVM). Thirty-three animals were administered FSH and hCG, and oocytes were collected 3, 6, 12, or 28-30 hours after hCG (VVM). Main Outcome Measure(s): Nuclear maturation and microtubule scores of oocytes and actin and tubulin transzonal processes of cumulus cells. Embryo development was observed for VVM oocytes. Result(s): The rate of nuclear maturation was faster for IVM oocytes compared with VVM oocytes. Actin transzonal processes decreased 0-12 hours after hCG administration for VVM oocytes. Tubulin transzonal processes of IVM and VVM oocytes decreased from 0 to 24 hours and from 0 to 3 hours, respectively. Embryo development improved as VVM time increased. Conclusion(s): Nuclear maturation and remodeling of cumulus-oocyte complex structural components associated with in vitro maturation do not parallel those of oocyte maturation in vivo, indicating that in vitro culture conditions continue to be suboptimal.
KW - actin
KW - ovary
KW - Rhesus monkey
KW - spindle
KW - tubulin
KW - zona pellucida
UR - http://www.scopus.com/inward/record.url?scp=65049086946&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=65049086946&partnerID=8YFLogxK
U2 - 10.1016/j.fertnstert.2008.05.013
DO - 10.1016/j.fertnstert.2008.05.013
M3 - Article
C2 - 19108829
AN - SCOPUS:65049086946
VL - 91
SP - 2043
EP - 2050
JO - Fertility and Sterility
JF - Fertility and Sterility
SN - 0015-0282
IS - 5 SUPPL.
ER -