nSMase2 activation and trafficking are modulated by oxidative stress to induce apoptosis

Michal Levy, S. Sianna Castillo, Tzipora Goldkorn

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

We have previously shown that accumulation of ceramide, triggered by hydrogen peroxide (H2O2), induces apoptosis of human airway epithelial (HAE) cells. Under oxidant exposure, a lung sphingomyelinase (SMase) is activated and displays continued ceramide generation and pro-apoptotic signaling, thus leading to the pathological apoptosis that causes lung injury. In a search for a specific SMase that is modulated by oxidative stress, we recently cloned nSMase2 from monkey lung tissue and HAE cells. Here, we show that this nSMase2 is up-regulated by an oxidant (H2O2) and is inhibited by an antioxidant (glutathione (GSH)). Moreover, nSMase2 subcellular localization is governed by oxidant exposure, which leads to its preferential trafficking to the plasma membrane, where it generates ceramide and induces apoptosis. On the other hand, exposure to GSH results in nSMase2 trafficking to the nucleus, where it neither generates ceramide nor induces apoptosis.

Original languageEnglish (US)
Pages (from-to)900-905
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume344
Issue number3
DOIs
StatePublished - Jun 9 2006

Fingerprint

Oxidative stress
Ceramides
Oxidative Stress
Chemical activation
Oxidants
Apoptosis
Sphingomyelin Phosphodiesterase
Epithelial Cells
Lung
Lung Injury
Cell membranes
Hydrogen Peroxide
Haplorhini
Glutathione
Antioxidants
Display devices
Cell Membrane
Tissue

Keywords

  • Apoptosis
  • Ceramide
  • Glutathione
  • Hydrogen peroxide
  • Lung
  • Neutral sphingomyelinase 2

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

nSMase2 activation and trafficking are modulated by oxidative stress to induce apoptosis. / Levy, Michal; Castillo, S. Sianna; Goldkorn, Tzipora.

In: Biochemical and Biophysical Research Communications, Vol. 344, No. 3, 09.06.2006, p. 900-905.

Research output: Contribution to journalArticle

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N2 - We have previously shown that accumulation of ceramide, triggered by hydrogen peroxide (H2O2), induces apoptosis of human airway epithelial (HAE) cells. Under oxidant exposure, a lung sphingomyelinase (SMase) is activated and displays continued ceramide generation and pro-apoptotic signaling, thus leading to the pathological apoptosis that causes lung injury. In a search for a specific SMase that is modulated by oxidative stress, we recently cloned nSMase2 from monkey lung tissue and HAE cells. Here, we show that this nSMase2 is up-regulated by an oxidant (H2O2) and is inhibited by an antioxidant (glutathione (GSH)). Moreover, nSMase2 subcellular localization is governed by oxidant exposure, which leads to its preferential trafficking to the plasma membrane, where it generates ceramide and induces apoptosis. On the other hand, exposure to GSH results in nSMase2 trafficking to the nucleus, where it neither generates ceramide nor induces apoptosis.

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