NS21

Re-defined and modified supplement B27 for neuronal cultures

Yucui Chen, Beth Stevens, Jufang Chang, Jeffrey Milbrandt, Ben A. Barres, Johannes W Hell

Research output: Contribution to journalArticle

182 Citations (Scopus)

Abstract

In vitro culturing of primary neurons is a mainstay of neurobiological research. Many of these culture paradigms have taken advantage of defined culture media rather than serum additives that contain undefined survival factors to facilitate experimental manipulations and interpretation of the results. To culture neurons in the absence of serum, defined supplements such as B27 are now widely used. However, commercially available supplements exhibit large variability in their capabilities to support neurons in culture. We re-optimized and modified earlier published formulations of B27 using 21 different ingredients (NS21). NS21 supports neuronal cultures of high quality as manifested by their morphological characteristics, formation of synapses, and postsynaptic responses. Much of the variability in the quality of B27/NS21 was due to variability in the quality of different sources of bovine serum albumin. Furthermore, we found that holo-transferrin used in NS21 is preferable over apo-transferrin used in B27 for the quality of neuronal cultures.

Original languageEnglish (US)
Pages (from-to)239-247
Number of pages9
JournalJournal of Neuroscience Methods
Volume171
Issue number2
DOIs
StatePublished - Jun 30 2008
Externally publishedYes

Fingerprint

Transferrin
Neurons
Bovine Serum Albumin
Serum
Synapses
Culture Media
Research
In Vitro Techniques

Keywords

  • B27 supplement
  • Dorsal root ganglion cells
  • DRG
  • Hippocampal neurons
  • Neuronal cultures
  • NS21 supplement
  • Primary hippocampal cultures
  • Retinal ganglion cells
  • RGC

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

NS21 : Re-defined and modified supplement B27 for neuronal cultures. / Chen, Yucui; Stevens, Beth; Chang, Jufang; Milbrandt, Jeffrey; Barres, Ben A.; Hell, Johannes W.

In: Journal of Neuroscience Methods, Vol. 171, No. 2, 30.06.2008, p. 239-247.

Research output: Contribution to journalArticle

Chen, Yucui ; Stevens, Beth ; Chang, Jufang ; Milbrandt, Jeffrey ; Barres, Ben A. ; Hell, Johannes W. / NS21 : Re-defined and modified supplement B27 for neuronal cultures. In: Journal of Neuroscience Methods. 2008 ; Vol. 171, No. 2. pp. 239-247.
@article{76c694277e2a4816b2644fc3b5a185eb,
title = "NS21: Re-defined and modified supplement B27 for neuronal cultures",
abstract = "In vitro culturing of primary neurons is a mainstay of neurobiological research. Many of these culture paradigms have taken advantage of defined culture media rather than serum additives that contain undefined survival factors to facilitate experimental manipulations and interpretation of the results. To culture neurons in the absence of serum, defined supplements such as B27 are now widely used. However, commercially available supplements exhibit large variability in their capabilities to support neurons in culture. We re-optimized and modified earlier published formulations of B27 using 21 different ingredients (NS21). NS21 supports neuronal cultures of high quality as manifested by their morphological characteristics, formation of synapses, and postsynaptic responses. Much of the variability in the quality of B27/NS21 was due to variability in the quality of different sources of bovine serum albumin. Furthermore, we found that holo-transferrin used in NS21 is preferable over apo-transferrin used in B27 for the quality of neuronal cultures.",
keywords = "B27 supplement, Dorsal root ganglion cells, DRG, Hippocampal neurons, Neuronal cultures, NS21 supplement, Primary hippocampal cultures, Retinal ganglion cells, RGC",
author = "Yucui Chen and Beth Stevens and Jufang Chang and Jeffrey Milbrandt and Barres, {Ben A.} and Hell, {Johannes W}",
year = "2008",
month = "6",
day = "30",
doi = "10.1016/j.jneumeth.2008.03.013",
language = "English (US)",
volume = "171",
pages = "239--247",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - NS21

T2 - Re-defined and modified supplement B27 for neuronal cultures

AU - Chen, Yucui

AU - Stevens, Beth

AU - Chang, Jufang

AU - Milbrandt, Jeffrey

AU - Barres, Ben A.

AU - Hell, Johannes W

PY - 2008/6/30

Y1 - 2008/6/30

N2 - In vitro culturing of primary neurons is a mainstay of neurobiological research. Many of these culture paradigms have taken advantage of defined culture media rather than serum additives that contain undefined survival factors to facilitate experimental manipulations and interpretation of the results. To culture neurons in the absence of serum, defined supplements such as B27 are now widely used. However, commercially available supplements exhibit large variability in their capabilities to support neurons in culture. We re-optimized and modified earlier published formulations of B27 using 21 different ingredients (NS21). NS21 supports neuronal cultures of high quality as manifested by their morphological characteristics, formation of synapses, and postsynaptic responses. Much of the variability in the quality of B27/NS21 was due to variability in the quality of different sources of bovine serum albumin. Furthermore, we found that holo-transferrin used in NS21 is preferable over apo-transferrin used in B27 for the quality of neuronal cultures.

AB - In vitro culturing of primary neurons is a mainstay of neurobiological research. Many of these culture paradigms have taken advantage of defined culture media rather than serum additives that contain undefined survival factors to facilitate experimental manipulations and interpretation of the results. To culture neurons in the absence of serum, defined supplements such as B27 are now widely used. However, commercially available supplements exhibit large variability in their capabilities to support neurons in culture. We re-optimized and modified earlier published formulations of B27 using 21 different ingredients (NS21). NS21 supports neuronal cultures of high quality as manifested by their morphological characteristics, formation of synapses, and postsynaptic responses. Much of the variability in the quality of B27/NS21 was due to variability in the quality of different sources of bovine serum albumin. Furthermore, we found that holo-transferrin used in NS21 is preferable over apo-transferrin used in B27 for the quality of neuronal cultures.

KW - B27 supplement

KW - Dorsal root ganglion cells

KW - DRG

KW - Hippocampal neurons

KW - Neuronal cultures

KW - NS21 supplement

KW - Primary hippocampal cultures

KW - Retinal ganglion cells

KW - RGC

UR - http://www.scopus.com/inward/record.url?scp=43449130057&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=43449130057&partnerID=8YFLogxK

U2 - 10.1016/j.jneumeth.2008.03.013

DO - 10.1016/j.jneumeth.2008.03.013

M3 - Article

VL - 171

SP - 239

EP - 247

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

IS - 2

ER -