NS21: Re-defined and modified supplement B27 for neuronal cultures

Yucui Chen, Beth Stevens, Jufang Chang, Jeffrey Milbrandt, Ben A. Barres, Johannes W Hell

Research output: Contribution to journalArticle

197 Scopus citations

Abstract

In vitro culturing of primary neurons is a mainstay of neurobiological research. Many of these culture paradigms have taken advantage of defined culture media rather than serum additives that contain undefined survival factors to facilitate experimental manipulations and interpretation of the results. To culture neurons in the absence of serum, defined supplements such as B27 are now widely used. However, commercially available supplements exhibit large variability in their capabilities to support neurons in culture. We re-optimized and modified earlier published formulations of B27 using 21 different ingredients (NS21). NS21 supports neuronal cultures of high quality as manifested by their morphological characteristics, formation of synapses, and postsynaptic responses. Much of the variability in the quality of B27/NS21 was due to variability in the quality of different sources of bovine serum albumin. Furthermore, we found that holo-transferrin used in NS21 is preferable over apo-transferrin used in B27 for the quality of neuronal cultures.

Original languageEnglish (US)
Pages (from-to)239-247
Number of pages9
JournalJournal of Neuroscience Methods
Volume171
Issue number2
DOIs
StatePublished - Jun 30 2008
Externally publishedYes

Keywords

  • B27 supplement
  • Dorsal root ganglion cells
  • DRG
  • Hippocampal neurons
  • Neuronal cultures
  • NS21 supplement
  • Primary hippocampal cultures
  • Retinal ganglion cells
  • RGC

ASJC Scopus subject areas

  • Neuroscience(all)

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