Novel ultra-fast deconvolution method for fluorescence lifetime imaging microscopy based on the laguerre expansion technique

J. A. Jo, Q. Fang, T. Papaioannou, Laura Marcu

Research output: Chapter in Book/Report/Conference proceedingConference contribution

10 Scopus citations

Abstract

A new deconvolution method for fluorescence lifetime imaging microspopy (FLIM) based on the Laguerre expansion technique is presented. The performance of this method was tested on synthetic FLIM images derived from a multiexponential model and from fluorescence lifetime standards, and then compared to standard algorithms of FLIM analysis. Our results demonstrated significant advantages of the Laguerre method over standard algorithms. First, the fluorescence intensity decays of arbitrary form can be estimated at every pixel, without a priori assumption of its functional form. Second, the number of delayed images required to perform deconvolution is relatively low (as low as 5), reducing the acquisition time. Third, ultra-fast light sources are not longer required, making less expensive to perform lifetime imaging. Finally and most important, deconvolution at every pixel is performed in parallel using a common Laguerre basis, thus allowing reducing significantly the computation time (i.e. synthetic 600×600 pixel images can be deconvolved with high accuracy in less than 20 s). Based on these findings, we believe that the Laguerre deconvolution technique represents a more robust and extremely fast analytical method that will allow exploring FLIM in practical real-time applications, such as clinical diagnosis.

Original languageEnglish (US)
Title of host publicationAnnual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings
Pages1271-1274
Number of pages4
Volume26 II
StatePublished - 2004
EventConference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004 - San Francisco, CA, United States
Duration: Sep 1 2004Sep 5 2004

Other

OtherConference Proceedings - 26th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2004
CountryUnited States
CitySan Francisco, CA
Period9/1/049/5/04

Keywords

  • Fluorescence lifetime imaging microscopy (FLIM)
  • Image analysis
  • Laguerre expansion technique

ASJC Scopus subject areas

  • Bioengineering

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    Jo, J. A., Fang, Q., Papaioannou, T., & Marcu, L. (2004). Novel ultra-fast deconvolution method for fluorescence lifetime imaging microscopy based on the laguerre expansion technique. In Annual International Conference of the IEEE Engineering in Medicine and Biology - Proceedings (Vol. 26 II, pp. 1271-1274)