Novel thermo-sensitive core-shell nanoparticles for targeted paclitaxel delivery

Yuanpei Li, Shirong Pan, Wei Zhang, Zhuo Du

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Novel thermo-sensitive nanoparticles self-assembled from poly(N,N-diethylacrylamide- co-acrylamide)-block-poly(γ-benzyl L-glutamate) were designed for targeted drug delivery in localized hyperthermia. The lower critical solution temperature (LCST) of nanoparticles was adjusted to a level between physiological body temperature (37 °C) and that used in local hyperthermia (about 43 °C). The temperature-dependent performances of the core-shell nanoparticles were systemically studied by nuclear magnetic resonance (NMR), circular dichroism (CD), fluorescence spectroscopy, dynamic light scattering (DLS), and atom force microscopy (AFM). The mean diameter of the nanoparticles increased slightly from 110 to 129nm when paclitaxel (PTX), a poorly water-soluble anti-tumor drug, was encapsulated. Astability study in bovine serum albumin (BSA) solution indicated that the PTX loaded nanoparticles may have a long circulation time under physiological environments as the LCST was above physiological body temperature and the shell remained hydrophilic at 37 °C. The PTX release profiles showed thermo-sensitive controlled behavior. The proliferation inhibiting activity of PTX loaded nanoparticles was evaluated against Hela cells in vitro, compared with Taxol (a formulation of paclitaxel dissolved in Cremophor EL and ethanol). The cytotoxicity of PTX loaded nanoparticles increased obviously when hyperthermia was performed. The nanoparticles synthesized here could be an ideal candidate for thermal triggered anti-tumor PTX delivery system.

Original languageEnglish (US)
Article number065104
JournalNanotechnology
Volume20
Issue number6
DOIs
StatePublished - 2009
Externally publishedYes

Fingerprint

Paclitaxel
Nanoparticles
Tumors
Temperature
Acrylamide
Fluorescence spectroscopy
Dichroism
Dynamic light scattering
Cytotoxicity
Bovine Serum Albumin
Microscopic examination
Ethanol
Nuclear magnetic resonance
Atoms
Water
Pharmaceutical Preparations

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Electrical and Electronic Engineering
  • Mechanical Engineering
  • Mechanics of Materials
  • Materials Science(all)

Cite this

Novel thermo-sensitive core-shell nanoparticles for targeted paclitaxel delivery. / Li, Yuanpei; Pan, Shirong; Zhang, Wei; Du, Zhuo.

In: Nanotechnology, Vol. 20, No. 6, 065104, 2009.

Research output: Contribution to journalArticle

Li, Yuanpei ; Pan, Shirong ; Zhang, Wei ; Du, Zhuo. / Novel thermo-sensitive core-shell nanoparticles for targeted paclitaxel delivery. In: Nanotechnology. 2009 ; Vol. 20, No. 6.
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