Novel role for a Saccharomyces cerevisiae nucleoporin, Nup170p, in chromosome segregation

O. Kerscher, P. Hieter, Mark Winey, M. A. Basrai

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

We determined that a mutation in the nucleoporin gene NUP170 leads to defects in chromosome transmission fidelity (ctf) and kinetochore integrity in Saccharomyces cerevisiae. A ctf mutant strain, termed s141, shows a transcription readthrough phenotype and stabilizes a dicentric chromosome fragment in two assays for kinetochore integrity. Previously, these assays led to the identification of two essential kinetochore components, Ctf13p and Ctf14p. Thus, s141 represents another ctf mutant involved in the maintenance of kinetochore integrity. We cloned and mapped the gene complementing the ctf mutation of s141 and showed that it is identical to the S. cerevisiae NUP170 gene. A deletion strain of NUP170 (nup17Oδ::HIS3) has a Ctf- phenotype similar to the s141 mutant (nup170-141) and also exhibits a kinetochore integrity defect. We identified a second nucleoporin, NUP157, a homologue of NUP170, as a suppressor of the Ctf- phenotype of nup170-141 and nup170δ::HIS3 strains. However, a deletion of NUP157 or several other nucleoporins did not affect chromosome segregation. Our data suggest that NUP170 encodes a specialized nucleoporin with a unique role in chromosome segregation and possibly kinetochore function.

Original languageEnglish (US)
Pages (from-to)1543-1553
Number of pages11
JournalGenetics
Volume157
Issue number4
StatePublished - Apr 25 2001
Externally publishedYes

Fingerprint

Nuclear Pore Complex Proteins
Kinetochores
Chromosome Segregation
Saccharomyces cerevisiae
Chromosomes
Phenotype
Genes
Mutation
Maintenance

ASJC Scopus subject areas

  • Genetics

Cite this

Kerscher, O., Hieter, P., Winey, M., & Basrai, M. A. (2001). Novel role for a Saccharomyces cerevisiae nucleoporin, Nup170p, in chromosome segregation. Genetics, 157(4), 1543-1553.

Novel role for a Saccharomyces cerevisiae nucleoporin, Nup170p, in chromosome segregation. / Kerscher, O.; Hieter, P.; Winey, Mark; Basrai, M. A.

In: Genetics, Vol. 157, No. 4, 25.04.2001, p. 1543-1553.

Research output: Contribution to journalArticle

Kerscher, O, Hieter, P, Winey, M & Basrai, MA 2001, 'Novel role for a Saccharomyces cerevisiae nucleoporin, Nup170p, in chromosome segregation', Genetics, vol. 157, no. 4, pp. 1543-1553.
Kerscher, O. ; Hieter, P. ; Winey, Mark ; Basrai, M. A. / Novel role for a Saccharomyces cerevisiae nucleoporin, Nup170p, in chromosome segregation. In: Genetics. 2001 ; Vol. 157, No. 4. pp. 1543-1553.
@article{d376042bf5924ffa87472c15b6a5fe13,
title = "Novel role for a Saccharomyces cerevisiae nucleoporin, Nup170p, in chromosome segregation",
abstract = "We determined that a mutation in the nucleoporin gene NUP170 leads to defects in chromosome transmission fidelity (ctf) and kinetochore integrity in Saccharomyces cerevisiae. A ctf mutant strain, termed s141, shows a transcription readthrough phenotype and stabilizes a dicentric chromosome fragment in two assays for kinetochore integrity. Previously, these assays led to the identification of two essential kinetochore components, Ctf13p and Ctf14p. Thus, s141 represents another ctf mutant involved in the maintenance of kinetochore integrity. We cloned and mapped the gene complementing the ctf mutation of s141 and showed that it is identical to the S. cerevisiae NUP170 gene. A deletion strain of NUP170 (nup17Oδ::HIS3) has a Ctf- phenotype similar to the s141 mutant (nup170-141) and also exhibits a kinetochore integrity defect. We identified a second nucleoporin, NUP157, a homologue of NUP170, as a suppressor of the Ctf- phenotype of nup170-141 and nup170δ::HIS3 strains. However, a deletion of NUP157 or several other nucleoporins did not affect chromosome segregation. Our data suggest that NUP170 encodes a specialized nucleoporin with a unique role in chromosome segregation and possibly kinetochore function.",
author = "O. Kerscher and P. Hieter and Mark Winey and Basrai, {M. A.}",
year = "2001",
month = "4",
day = "25",
language = "English (US)",
volume = "157",
pages = "1543--1553",
journal = "Genetics",
issn = "0016-6731",
publisher = "Genetics Society of America",
number = "4",

}

TY - JOUR

T1 - Novel role for a Saccharomyces cerevisiae nucleoporin, Nup170p, in chromosome segregation

AU - Kerscher, O.

AU - Hieter, P.

AU - Winey, Mark

AU - Basrai, M. A.

PY - 2001/4/25

Y1 - 2001/4/25

N2 - We determined that a mutation in the nucleoporin gene NUP170 leads to defects in chromosome transmission fidelity (ctf) and kinetochore integrity in Saccharomyces cerevisiae. A ctf mutant strain, termed s141, shows a transcription readthrough phenotype and stabilizes a dicentric chromosome fragment in two assays for kinetochore integrity. Previously, these assays led to the identification of two essential kinetochore components, Ctf13p and Ctf14p. Thus, s141 represents another ctf mutant involved in the maintenance of kinetochore integrity. We cloned and mapped the gene complementing the ctf mutation of s141 and showed that it is identical to the S. cerevisiae NUP170 gene. A deletion strain of NUP170 (nup17Oδ::HIS3) has a Ctf- phenotype similar to the s141 mutant (nup170-141) and also exhibits a kinetochore integrity defect. We identified a second nucleoporin, NUP157, a homologue of NUP170, as a suppressor of the Ctf- phenotype of nup170-141 and nup170δ::HIS3 strains. However, a deletion of NUP157 or several other nucleoporins did not affect chromosome segregation. Our data suggest that NUP170 encodes a specialized nucleoporin with a unique role in chromosome segregation and possibly kinetochore function.

AB - We determined that a mutation in the nucleoporin gene NUP170 leads to defects in chromosome transmission fidelity (ctf) and kinetochore integrity in Saccharomyces cerevisiae. A ctf mutant strain, termed s141, shows a transcription readthrough phenotype and stabilizes a dicentric chromosome fragment in two assays for kinetochore integrity. Previously, these assays led to the identification of two essential kinetochore components, Ctf13p and Ctf14p. Thus, s141 represents another ctf mutant involved in the maintenance of kinetochore integrity. We cloned and mapped the gene complementing the ctf mutation of s141 and showed that it is identical to the S. cerevisiae NUP170 gene. A deletion strain of NUP170 (nup17Oδ::HIS3) has a Ctf- phenotype similar to the s141 mutant (nup170-141) and also exhibits a kinetochore integrity defect. We identified a second nucleoporin, NUP157, a homologue of NUP170, as a suppressor of the Ctf- phenotype of nup170-141 and nup170δ::HIS3 strains. However, a deletion of NUP157 or several other nucleoporins did not affect chromosome segregation. Our data suggest that NUP170 encodes a specialized nucleoporin with a unique role in chromosome segregation and possibly kinetochore function.

UR - http://www.scopus.com/inward/record.url?scp=0035044364&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035044364&partnerID=8YFLogxK

M3 - Article

C2 - 11290711

AN - SCOPUS:0035044364

VL - 157

SP - 1543

EP - 1553

JO - Genetics

JF - Genetics

SN - 0016-6731

IS - 4

ER -