Novel poly(ethylene-co-acrylic acid) nanofibrous biomaterials for peptide synthesis and biomedical applications

Bei Xiang, Gang Sun, Kit Lam, Kai Xiao

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Poly(ethylene-co-acrylic acid) (PE-co-AA) fibers in sizes of 200-500 nm were prepared by using a novel melt-extrusion-extraction fabrication process. The thermoplastic nanofibers could be controllably dispersed and reassembled by a novel solvent exchange filtration method. The dispersed PE-co-AA nanofibers possess active surface areas and could directly conduct chemical reactions on surfaces. Surface modifications and organic synthesis on the nanofibers were proven effective and controllable after the dispersion. Multistep synthesis of biomolecules, such as peptide ligand HWRGWV against Fc portion of human IgG, was successful. The surface-anchored ligand has shown bioactivity through selective binding to and staining by human IgG-alkaline phosphatase conjugate. Another peptide, LXY3, a selective cyclic peptide ligand against a3b1 integrin of MDA-MB-231 breast cancer cells, was also prepared on the surfaces of the dispersed nanofibers. The results showed that MDAMB-231 cells were able to specifically bind to and grow on surfaces of the nanofibers that were functionalized with LXY3.

Original languageEnglish (US)
Pages (from-to)245-255
Number of pages11
JournalJournal of Biomedical Materials Research - Part A
Volume95
Issue number1
DOIs
StatePublished - Oct 2010

Keywords

  • Cell binding
  • Nanofiber
  • Peptide synthesis
  • Poly(ethylene-co-acrylic acid)
  • Solid phase synthesis

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Ceramics and Composites
  • Metals and Alloys
  • Medicine(all)

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