Abstract
Poly(ethylene-co-acrylic acid) (PE-co-AA) fibers in sizes of 200-500 nm were prepared by using a novel melt-extrusion-extraction fabrication process. The thermoplastic nanofibers could be controllably dispersed and reassembled by a novel solvent exchange filtration method. The dispersed PE-co-AA nanofibers possess active surface areas and could directly conduct chemical reactions on surfaces. Surface modifications and organic synthesis on the nanofibers were proven effective and controllable after the dispersion. Multistep synthesis of biomolecules, such as peptide ligand HWRGWV against Fc portion of human IgG, was successful. The surface-anchored ligand has shown bioactivity through selective binding to and staining by human IgG-alkaline phosphatase conjugate. Another peptide, LXY3, a selective cyclic peptide ligand against a3b1 integrin of MDA-MB-231 breast cancer cells, was also prepared on the surfaces of the dispersed nanofibers. The results showed that MDAMB-231 cells were able to specifically bind to and grow on surfaces of the nanofibers that were functionalized with LXY3.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 245-255 |
| Number of pages | 11 |
| Journal | Journal of Biomedical Materials Research - Part A |
| Volume | 95 |
| Issue number | 1 |
| DOIs | |
| State | Published - Oct 2010 |
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Keywords
- Cell binding
- Nanofiber
- Peptide synthesis
- Poly(ethylene-co-acrylic acid)
- Solid phase synthesis
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
- Ceramics and Composites
- Metals and Alloys
- Medicine(all)
Cite this
Novel poly(ethylene-co-acrylic acid) nanofibrous biomaterials for peptide synthesis and biomedical applications. / Xiang, Bei; Sun, Gang; Lam, Kit; Xiao, Kai.
In: Journal of Biomedical Materials Research - Part A, Vol. 95, No. 1, 10.2010, p. 245-255.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Novel poly(ethylene-co-acrylic acid) nanofibrous biomaterials for peptide synthesis and biomedical applications
AU - Xiang, Bei
AU - Sun, Gang
AU - Lam, Kit
AU - Xiao, Kai
PY - 2010/10
Y1 - 2010/10
N2 - Poly(ethylene-co-acrylic acid) (PE-co-AA) fibers in sizes of 200-500 nm were prepared by using a novel melt-extrusion-extraction fabrication process. The thermoplastic nanofibers could be controllably dispersed and reassembled by a novel solvent exchange filtration method. The dispersed PE-co-AA nanofibers possess active surface areas and could directly conduct chemical reactions on surfaces. Surface modifications and organic synthesis on the nanofibers were proven effective and controllable after the dispersion. Multistep synthesis of biomolecules, such as peptide ligand HWRGWV against Fc portion of human IgG, was successful. The surface-anchored ligand has shown bioactivity through selective binding to and staining by human IgG-alkaline phosphatase conjugate. Another peptide, LXY3, a selective cyclic peptide ligand against a3b1 integrin of MDA-MB-231 breast cancer cells, was also prepared on the surfaces of the dispersed nanofibers. The results showed that MDAMB-231 cells were able to specifically bind to and grow on surfaces of the nanofibers that were functionalized with LXY3.
AB - Poly(ethylene-co-acrylic acid) (PE-co-AA) fibers in sizes of 200-500 nm were prepared by using a novel melt-extrusion-extraction fabrication process. The thermoplastic nanofibers could be controllably dispersed and reassembled by a novel solvent exchange filtration method. The dispersed PE-co-AA nanofibers possess active surface areas and could directly conduct chemical reactions on surfaces. Surface modifications and organic synthesis on the nanofibers were proven effective and controllable after the dispersion. Multistep synthesis of biomolecules, such as peptide ligand HWRGWV against Fc portion of human IgG, was successful. The surface-anchored ligand has shown bioactivity through selective binding to and staining by human IgG-alkaline phosphatase conjugate. Another peptide, LXY3, a selective cyclic peptide ligand against a3b1 integrin of MDA-MB-231 breast cancer cells, was also prepared on the surfaces of the dispersed nanofibers. The results showed that MDAMB-231 cells were able to specifically bind to and grow on surfaces of the nanofibers that were functionalized with LXY3.
KW - Cell binding
KW - Nanofiber
KW - Peptide synthesis
KW - Poly(ethylene-co-acrylic acid)
KW - Solid phase synthesis
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UR - http://www.scopus.com/inward/citedby.url?scp=77956486924&partnerID=8YFLogxK
U2 - 10.1002/jbm.a.32819
DO - 10.1002/jbm.a.32819
M3 - Article
C2 - 20597124
AN - SCOPUS:77956486924
VL - 95
SP - 245
EP - 255
JO - Journal of Biomedical Materials Research - Part A
JF - Journal of Biomedical Materials Research - Part A
SN - 1549-3296
IS - 1
ER -