Novel picornavirus in turkey poults with hepatitis, California, USA

Kirsi S. Honkavuori, H L Shivaprasad, Thomas Briese, Craig Street, David L. Hirschberg, Stephen K. Hutchison, W. Ian Lipkin

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

To identify a candidate etiologic agent for turkey viral hepatitis, we analyzed samples from diseased turkey poults from 8 commercial fl ocks in California, USA, that were collected during 2008-2010. High-throughput pyrosequencing of RNA from livers of poults with turkey viral hepatitis (TVH) revealed picornavirus sequences. Subsequent cloning of the ≈9-kb genome showed an organization similar to that of picornaviruses with conservation of motifs within the P1, P2, and P3 genome regions, but also unique features, including a 1.2-kb sequence of unknown function at the junction of P1 and P2 regions. Real-time PCR confi rmed viral RNA in liver, bile, intestine, serum, and cloacal swab specimens from diseased poults. Analysis of liver by in situ hybridization with viral probes and immunohistochemical testing of serum demonstrated viral nucleic acid and protein in livers of diseased poults. Molecular, anatomic, and immunologic evidence suggests that TVH is caused by a novel picornavirus, tentatively named turkey hepatitis virus.

Original languageEnglish (US)
Pages (from-to)480-487
Number of pages8
JournalEmerging Infectious Diseases
Volume17
Issue number3
DOIs
StatePublished - Mar 2011

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases
  • Epidemiology

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    Honkavuori, K. S., Shivaprasad, H. L., Briese, T., Street, C., Hirschberg, D. L., Hutchison, S. K., & Ian Lipkin, W. (2011). Novel picornavirus in turkey poults with hepatitis, California, USA. Emerging Infectious Diseases, 17(3), 480-487. https://doi.org/10.3201/eid1703.101410